鬼笔环肽CruzFluor™405偶联物

鬼笔环肽CruzFluor™405偶联物是一种蓝色荧光染料，是405的极佳替代品。鬼笔环肽是由死伞蘑菇[鹅膏菌]生产的七肽鬼笔毒素。鬼笔环肽结合并稳定丝状F-肌动蛋白，防止成对的丝发生解离，并干扰细胞中丝状（聚合）肌动蛋白和球状（单体）肌动蛋白之间的自然平衡，并抑制F-肌动蛋白的ATP水解活性。肌动蛋白被细胞用于机械过程，例如生长，细胞骨架的支架和运动，并且这种肌动蛋白平衡的破坏破坏了细胞功能。肌动蛋白蛋白质在不同物种之间的差异不超过20％。|鬼笔环肽的这些特性使鬼笔环肽CruzFluor™成为标记，鉴定

Phalloidin CruzFluor™ 405 Conjugate is a blue fluorescent dye that is an excellent substitute for AlexFluor® 405. Phalloidin is a heptapeptide phallotoxin produced by|Amanita phalloides|, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.|These properties of Phalloidin make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications.
Phalloidin CruzFluor™ conjugates can be used alongside antibodies in IF staining of formalin-fixed cells and formalin-fixed, frozen tissue sections. The Phalloidin will stain F-actin.1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMS
Phalloidin CruzFluor™ 405 Conjugateis a fluorescent phalloidin conjugate that selectively binds to F-actins