Hotstart HiTaq PCR Mix（2×）

产品说明

本试剂提供了进行简便、灵敏的 PCR 检测系统。核心试剂包含了经过优化的缓冲液、dNTPs Mix、化学修饰热启动酶（Hotstart HiTaq DNA polymerase）和 MgCl2 溶液，使用者只需加入适量的引物和探针或荧光染料，即可进行荧光 PCR 检测。在反应中 Hotstart HiTaq DNA polymerase 的加入使得反应具有极强的特异性和高度灵敏度。

产品内容

Hotstart HiTaq PCR Mix

使用方法

1.PCR 反应体系的设置

a.溶解并混匀 PCR 反应所需的各种溶液，并放置于冰浴上或冰盒内。建议反应 PCR 液体分装使用， 避免反复冻融。

b.参考下表设置 PCR 反应，建议 PCR 反应体系的配置在冰浴或在冰盒上进行：

※ 注：引物、探针以及模板的用量根据实验用量加入。

c.用移液器轻轻吹打混匀或轻微 Vortex 混匀，室温离心数秒，使液体积聚于管底。

d.各设置好的 PCR 反应管置于 PCR 仪上，开始 PCR 反应。

2.实验设置

注：Hotstart HiTaq DNA Polymer的热激时间10min到15min都可以，所以第二步的95℃、10 min（热启动酶热激）的时间可以根据实验要求进行设置，使用时时间从10min到15min设置都可以，比较灵活。另收集荧光设置为两步或者三步可以根据您的实验设置自行设定。

Product manual

This reagent provides a simple and sensitive PCR detection system. The core reagents include optimized buffers, dNTPs Mix, chemically modified Hotstart HiTaq DNA polymerase and MgCl2 solution. Users only need to add appropriate primers and probes or fluorescent dyes to perform fluorescent PCR detection. The addition of Hotstart HiTaq DNA polymerase in the reaction makes the reaction highly specific and highly sensitive.

Product content

Hotstart HiTaq PCR Mix

Instructions

1. PCR reaction system settings

a. Dissolve and mix the various solutions required for the PCR reaction, and place them on an ice bath or in an ice box. It is recommended that the reaction PCR liquid be used in aliquots to avoid repeated freezing and thawing.

b. Refer to the following table to set up the PCR reaction. It is recommended to configure the PCR reaction system in an ice bath or on an ice box:

※ Note: The amount of primers, probes and templates are added according to the amount of experiment.

c. Use a pipette to mix gently or gently Vortex to mix, and centrifuge for a few seconds at room temperature to allow the liquid to accumulate at the bottom of the tube.

d. Place each set of PCR reaction tubes on the PCR machine to start the PCR reaction.

2. Experimental settings

Note: The heat shock time of Hotstart HiTaq DNA Polymer can be 10min to 15min, so the 95℃, 10min (hot start enzyme heat shock) time of the second step can be set according to the experimental requirements, and the time of use can be set from 10min to 15min It’s ok, it’s more flexible. In addition, the collection of fluorescence is set to two or three steps, which can be set according to your experimental settings.