5-(and-6)-Carboxyrhodamine 6G, hydrochloride

Product description

Ex(nm) : 519

Em(nm) : 544

Solvent：DMSO

MW : 494.97

Features and Biological Applications

5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5(6)-CR6G, SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications.

References

1. Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci USA, 2005, 102, 5926-31.

2.  Hung, S.C., et al., Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores. Anal Biochem,1996, 238, 165-70.

Product description

Ex(nm) : 519

Em(nm) : 544

Solvent：DMSO

MW : 494.97

Features and Biological Applications

5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5(6)-CR6G, SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications.

References

1. Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci USA, 2005, 102, 5926-31.

2.  Hung, S.C., et al., Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores. Anal Biochem,1996, 238, 165-70.