6-Carboxyrhodamine 6G, hydrochloride

Product description

Ex(nm) : 520

Em(nm) : 547

Solvent：DMSO

MW : 494.97

Features and Biological Applications

6-CR6G is the other purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates.  Complimentary to 5-CR6G, 6-CR6G is predominantly used for labeling nucleotides and nucleic acids.

References

1.Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides.Proc Natl Acad Sci U S A2005, 102, 5926-31.

2. Dietrich, A., et al., Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: A comparative study of ensemble and single-molecule data.J Biotechnol2002, 82, 211-31.

3. Arezi, B., et al., Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant.J Mol Biol2002, 322, 719-29.

4. Hung SC, et al. (1997). Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem252, 78-88.

Product description

Ex(nm) : 520

Em(nm) : 547

Solvent：DMSO

MW : 494.97

Features and Biological Applications

6-CR6G is the other purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates.  Complimentary to 5-CR6G, 6-CR6G is predominantly used for labeling nucleotides and nucleic acids.

References

1.Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides.Proc Natl Acad Sci U S A2005, 102, 5926-31.

2. Dietrich, A., et al., Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: A comparative study of ensemble and single-molecule data.J Biotechnol2002, 82, 211-31.

3. Arezi, B., et al., Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant.J Mol Biol2002, 322, 719-29.

4. Hung SC, et al. (1997). Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem252, 78-88.