Super Fluor 750

Super Fluor 750 活化酯的性质参数

活化酯分子量: 1300
最大吸收波长(nm): 750
摩尔吸光系数(M-1cm-1): 240000 

最大发射波长(nm): 775
A280nm /Amax: 4%

原料准备

A．活性染料 1mg；B．碳酸氢钠 ～84mg；C．抗体纯化树脂P-30／Sephadex G25M／透析袋；D．空柱 5 个；E．收集管 5 个；

注意：纯化的蛋白buffer 中不得含有铵离子或伯铵，因为它们会与蛋白质竞争结合活性染料。不纯的抗体或者含BSA 或明胶的抗体标记效果不好。低浓度的NaN3(<3mM)或thimerosal（<1mM）不影响偶联效果。

活性染料稳定性与储存

未开封的粉末在避光干燥-20℃可存放6个月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用；无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 750 activated ester

Activated ester molecular weight: 1300
Maximum absorption wavelength (nm): 750
Molar absorption coefficient (M-1cm-1): 240000

Maximum emission wavelength (nm): 775
A280nm /Amax: 4%

Raw material preparation

A. Reactive dye 1mg; B. Sodium bicarbonate ~ 84mg; C. Antibody purification resin P-30/Sephadex G25M/dialysis bag; D. 5 empty columns; E. 5 collection tubes;

Note: The purified protein buffer must not contain ammonium ions or primary ammonium ions, because they will compete with the protein to bind reactive dyes. Impure antibodies or antibodies containing BSA or gelatin are not well labeled. Low concentration of NaN3 (<3mM) or thimerosal (<1mM) does not affect the coupling effect.

Reactive dye stability and storage

The unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.