2-AB Chitotriose – Quantitative

The 2-AB labeled chitotriose standard is a fluorescently tagged linear tri-N-acetylglucosamine quantitative standard. The standard has been accurately dispensed from a bulk solution the concentration of which has been determined by quantitative NMR.

The chitotriose quantatative standard is also available as an unlabeled glycan and 2-AA labeled. 

Accurate Quantity The bulk chitotriose concentration value was used to determine the amount of sample to be dispensed per vial to obtain 100 pmol of chitotriose per vial.  Once dispensed the amount of chitotriose per vial is determined by monosaccharide analysis. The dispensing error is predicted to be than less 5%.

Regulatory Submissions Support system suitability and drugs regulatory submission by demonstrating consistent and reproducible glycosylation levels

Reliable Quantification Method Follows established glycan analysis techniques. Provides data comparable to gold-standard glycoprofiling methods based on monosaccharide analysis
Quick and Easy Used in QC routine in-house to determine quantity of both bulk and dispensed glycans

Use as Internal or External Quantitative Standard The Chitotriose standard can be spiked directly into your glycan sample or run in parallel.

Use as an Internal Standard Our unlabeled chitotriose standard can be used as an internal standard to quantify glycans in your sample. To do this, add a known amount of the unlabeled chitotriose to your unlabeled glycan sample. The mixture is then fluorescently labeled, cleaned up and run on HPLC/UHPLC. The unknown quantity of the glycan can be inferred by comparison of the chitotriose and glycan peak areas. The advantage of this method is that any sample loss occurring during the labelling and clean up stages will be the same for the sample and the chitotriose, thereby removing this as a source of error. The results obtained when running this method compare favourably with monosaccharide testing.
Use as External Standards The 2-AB and 2-AA labeled standard can be used as external standards to quantify glycans; these standards can be run directly on the HPLC/UHPLC. Again, the quantity of glycans in your sample is inferred by comparison of the peak areas for chitotriose (known amount) and your sample. This method is useful when quantification of already labeled glycans is required. They are also useful when quantification of several glycan samples is required in the same run, as the labeled chitotriose standard can be used as external standard for all the samples.

HILIC UHPLC column chromatogram of 2-AB labeled chitotriose and NGA2F glycans.

BioQuant Chitotriose Workflow
Integrates easily With fluorescent labeling workflows without requiring any extra steps.

2-AB Labeled Chitotriose Data Analysis