异硫氰酸荧光素-葡聚糖

应用

用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

其他说明

已发现天然右旋糖酐的分子量 (MW) 在 900 万至 500 万范围内。较低分子量的右旋糖酐将表现出略微较少的支化，并具有更窄的分子量分布范围。分子量大于 10,000 的葡聚糖似乎是表现为高度支化的。 随着分子量的增加，葡聚糖分子的对称性增强。 分子量为2,000至10,000的葡聚糖分子表现出可膨胀线圈的特性。在分子量低于 2,000 时，右旋糖酐更像棒状。右旋糖酐的分子量用以下一种或多种方法测定：低角度激光散射、尺寸排阻色谱法、铜络合、和蒽酮试剂、显色还原末端糖测定和粘度。

制备说明

通常以25mg/ml或更高的浓度对FITC葡聚糖在水中的溶解度进行测试。 溶液应保证避光。 在体内，FITC-葡聚糖可保持稳定超过24小时。

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Other Notes

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.