NGA2F 聚糖

产品介绍：
G0F聚糖存在于包括人IgG在内的许多哺乳动物糖蛋白上，是双触角N连接寡糖（如A2F，A1F和NA2F）的亚结构，这些寡糖广泛存在于糖蛋白上。本品通常使用HPLC和糖苷酶消化的组合，通过肼分解从猪甲状腺球蛋白释放的寡糖池中纯化。

G0F聚糖是单克隆抗体上四种最常见的聚糖，以及FA2G1 Glycan和NA2F Glycan两张异构体。已知这些聚糖会影响抗体在体内的稳定性和溶解度，以及影响ADCC和CDC活性。由于这些聚糖在单克隆抗体上发现的生物学功能，这些寡糖的微异质性必须在药物开发和批准的生物药品的批次间差异中表征。

说明：

未标记聚糖和标记的聚糖可通过各种 HPLC（高压液相色谱）方法使用HPLC 色谱柱进行分离和分析。

色谱柱是一种特别强大的工具，用于纯化和分析来自复杂聚糖混合物的标记的低聚糖。聚糖也可以通过质谱、电泳和各种类型的光谱进行分析，请联系我们以获取有关您的特定应用的建议。

Product description：

The G0F glycan is found on many mammalian glycoproteins including human IgG and is a substructure of bi-antennary N-linked oligosaccharides such as A2F, A1F, and NA2F which are widely found on glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine thyroglobulin by hydrazinolysis using a combination of HPLC and glycosidase digestion.The G0F glycan is on of the four most common glycans found on monoclonal antibodies, along with the two FA2G1 Glycan isomers, and NA2F Glycan.
These glycans are known to affect stability and solubility of antibodies within the body, as well as affecting ADCC and CDC activity. Due to the biological functions of these glycans found on monoclonal antibodies, the micro-heterogeneity of these oligosaccharides must be characterized during drug development and lot-to-lot variability in approved biological drug products.

Explanation：

Unlabeled glycans and  labeled glycans may be separated and analysed by a variety of HPLC (high pressure liquid chromatography) methods using HPLC columns.

Columns are an especially powerful tool for the purification and analysis of labeled oligosaccharides from complex glycan mixtures. Please contact us for advise regarding your particular application.