异硫氰酸荧光素(异构体I)
基本信息
| 产品名称 | 异硫氰酸荧光素(异构体I) |
|---|---|
| 英文名称 | Fluorescein isothiocyanate isomer I |
| 别名 | 荧光素-5-异氰酸酯(异构体I);荧光素-异氰酸酯;异硫氰酸荧光素 |
| 英文别名 | 5-FITC (isomer I);5-Isothiocyanatofluorescein (isomer I);Fluorescein 5-isothiocyanate;FITC;Fluorescein isothiocyanate |
| 规格或纯度 | 90% |
| 运输条件 | 冰袋运输 |
| 生化机理 | Fluorescein isothiocyanate isomer I is an amine-reactive reagent for the FITC labeling of proteins, microsequencing of proteins, and peptides (HPLC). .Fluorescent marker. Amine reactive fluorescein isomer. Yields conjugates with superior stability compared to other FITC isomers. Donor for FRET assays. |
| 标签 | 光谱试剂,六元杂环化合物 |
一般描述
有吸湿性。能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失,微溶于丙酮、乙醚和石油醚。不溶于水。生化研究。荧光抗体示踪。病毒和寄生虫等所致疾病的快速诊断。
FITC (isomer I) is a derivative of fluorescein. FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. FITC can be easily used as labelling agent of various substrates such as proteins or polysaccharides. Isothiocyanate easily react with nucleophiles such as amines under mild conditions. FITC can also be used in flow cytometry.
Structure
Fluorescein isothiocyanate (widely known as FITC) is a derivative of fluorescein which contains an isothiocyanate group at either position 5 or position 6 of the bottom benzene ring.
Spectral data
FITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm.
Application
FITC has a wide-range of applications. Including fluorescent labelling of antibodies and flow cytometry. FITC-functionalized products e.g. FITC-dextrans are very useful for studying permeability and transport in a large variety of cells and tissues e.g. intestinal, neoplastic, and ocular tissues as well in research related to brain and neural system.
相关属性
| CAS编号和信息 | 3326-32-7 |
|---|---|
| MDL号 | MFCD00005063 |
| 品牌 | Jinpan |
| 溶解性 | 溶于DMSO和DMF。 |
| 敏感性 | 对光线,湿度和热敏感 |
| 储存温度 | 2-8°C储存,充氩 |
| 密度 | 1.54 |
| 备注 | 如果有可能，您尽量在同一天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。 |
| 分子式 | C21H11NO5S |
| IUPAC Name | {‘2.1.1 IUPAC Name’: [“3′,6′-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one |
| INCHI | InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| InChi Key | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| Canonical SMILES | C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O |
| PubChem CID | 18730 |
| 分子量 | 389.38 |
| Beilstein号 | 1407295 |
| Reaxy-Rn | 1407295 |
| 沸点 | ~708.6 °C at 760 mmHg |
| 熔点 | >360°C |
| Excitation(nm) | 495 |
| Emission(nm) | 519 |