CTLA4 / CD152蛋白
货 号：CT4-H5229
产品规格：100ug
原 产 地：
参考价格：1280 （参考价格，以实际价格为准）
优惠价格：
CTLA4 / CD152蛋白
Human CTLA-4 / CD152 Protein
分子别名（Synonym）
CTLA4,CD152
表达区间及表达系统（Source）
Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 – Phe 162 (Accession # NP_005205.2).
Predicted N-terminus: Ala 37
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
活性（Bioactivity）-ELISA
Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 1-6.4 ng/mL (Routinely tested).
活性（Bioactivity）-SPR
Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with an affinity constant of 25.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
产品详细信息
CTLA4 / CD152蛋白
Human CTLA-4 / CD152 Protein
分子别名（Synonym）
CTLA4,CD152
表达区间及表达系统（Source）
Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 – Phe 162 (Accession # NP_005205.2).
Predicted N-terminus: Ala 37
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
活性（Bioactivity）-ELISA
Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 1-6.4 ng/mL (Routinely tested).
活性（Bioactivity）-SPR
Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with an affinity constant of 25.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).