FITC Annexin V Apoptosis Detection Kit with 7-AAD
货 号:640922
产品规格:100T
原 产 地:USA
参考价格:2795 (参考价格,以实际价格为准)
优惠价格:2375
FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922 100T
Reactivity: All mammalian species
Storage & Handling: Store between 2°C and 8°C. Do not freeze.
Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:
Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.
产品详细信息
FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922 100T
Reactivity: All mammalian species
Storage & Handling: Store between 2°C and 8°C. Do not freeze.
Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:
Staining Procedure:
1. Wash cells twice with cold BioLegend’s Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.