乙氧基试卤灵
基本信息
| 产品名称 | 乙氧基试卤灵 |
|---|---|
| 英文名称 | Resorufin ethyl ether |
| 别名 | 7-乙氧基resorufin;7-ER |
| 英文别名 | Ethoxyresorufin; 7-Ethoxy-3H-phenoxazin-3-one; O7-Ethylresorufin; 7-Ethoxyresorufin; 7-ER; 7-Ethoxy Resorufin |
| 应用 | 7-Ethoxyresorufin (7-ER) is a substrate used in environmental toxicology studies to monitor ethoxyresorufin-O-deethylase catalytic activity in the EROD assay. The EROD assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. It provides evidence of receptor-mediated induction of cytochrome P450-dependant monooxygenases (the CYP1A subfamily specifically) by xenobiotic chemicals. |
| 规格或纯度 | ≥95% (UV) |
| 运输条件 | 超低温冰袋运输 |
| 生化机理 | 荧光微粒体脱烷基酶和CYP450底物。产生红色荧光染料试卤灵（Ex / Em 571/585 nm）。适用于xA0区分CYP同工酶1A1和1A2。 |
一般描述
7-Ethoxyresorufin is a fluorimetric substrate and suicide inhibitor of CYP (cytochrome P450). Also shown to inhibit several reductase enzymes, specifically CYPOR (NADPH-P450 reductase). The binding to CYP (cytochrome P450) is thoμght to inhibit the vasorelaxant response to acetylcholine. 7-Ethoxyresorufin has also demonstrated inhibition of NO synthesis by uncoupling neuronal NOS1 (nitric oxide synthase); it is a noncompetitive inhibitor of neuronal NOS1 with respect to L-arginine with a Ki value of 0.76 +/- 0.06 μM. 7-Ethoxyresorufin is a substate of CYP1A1.7-Ethoxyresorufin (7-ER) is a substrate used in environmental toxicology studies to monitor ethoxyresorufin-O-deethylase catalytic activity in the EROD assay. The EROD assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. It provides evidence of receptor-mediated induction of cytochrome P450-dependant monooxygenases (the CYP1A subfamily specifically) by xenobiotic chemicals.
7-Ethoxyresorufin is a fluorimetric substrate and suicide inhibitor of CYP (cytochrome P450). Also shown to inhibit several reductase enzymes, specifically CYPOR (NADPH-P450 reductase). The binding to CYP (cytochrome P450) is thought to inhibit the vasorelaxant response to acetylcholine. 7-Ethoxyresorufin has also demonstrated inhibition of NO synthesis by uncoupling neuronal NOS1 (nitric oxide synthase); it is a noncompetitive inhibitor of neuronal NOS1 with respect to L-arginine with a Ki value of 0.76 +/- 0.06 μM. 7-Ethoxyresorufin is a substate of CYP1A1.
7-Ethoxyresorufin (7-ER) is a substrate used in environmental toxicology studies to monitor ethoxyresorufin-O-deethylase catalytic activity in the EROD assay. The EROD assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widely used biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. It provides evidence of receptor-mediated induction of cytochrome P450-dependant monooxygenases (the CYP1A subfamily specifically) by xenobiotic chemicals.
相关属性
| CAS编号 | 5725-91-7 |
|---|---|
| 溶解性 | Soluble in DMSO (5 mg/mL), ethanol (5 mg/mL), chloroform, dichloromethane, DMF (5 mg/mL), alcohols, and methanol. Insoluble in water. |
| 储存温度 | -20°C储存 |
| MDL号 | MFCD00037661 |
| 分子量 | 241.24 |
| 分子式 | C14H11NO3 |
| 品牌 | Jinpan |
| 备注 | 有关更多信息，请参阅SDS。有关溶解度，用法和处理的更多建议？请访问我们的常见问题（FAQ）页面以获取更多详细信息。 |
| PubChem CID | 3294 |