Phospho-FAK2 (Tyr579, Tyr580) Polyclonal Antibody for Western Blot, IF, ICC, IHC (P) and Flow
Phospho-FAK2 (Tyr579, Tyr580) Antibody (44-636G) in IF
Immunofluorescence analysis of Phospho-FAK2 / PYK2 pTyr580 was done on 70% confluent log phase A549 cells treated with 50ng of PDGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-FAK2 / PYK2 pTyr580 Rabbit Polyclonal Antibody (44636G) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control. The images were captured at 60X magnification.
