Chromeo™P543

Chromeo™P543通过显示从紫色到深红色的颜色变化来检测蛋白质和多肽。与伯胺结合后，它经历了27nm的大短波光谱偏移，肉眼可见。量子产率的大幅度提高也将非荧光染料或仅弱荧光染料变成了明亮的荧光共轭物。吸收带和发射带的移动以及增加的荧光量子产率有效地消除了任何未结合染料的背景。此外，由于未结合的吡啶鎓染料在标记过程中会水解，因此无需经过简单的一步式室温孵育即可纯化。标记的蛋白质可立即使用，并且消除了未结合染料的任何可能背景。由于独特的缀合机制，用Py-Dyes标记的蛋白质可保持其天然电荷和等电点。由于

Chromeo™ P543 detects proteins and peptides by exhibiting a color change from violet to dark red. After binding to primary amines, it undergos a large shortwave spectral shift of 27nm that is visible to the naked eye. There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate. The shift of the absorption & emission bands together with the increased fluorescence quantum yield effectively eliminates background from any unbound dye. In addition, because unbound the pyrylium dye is hydrolyzed during the labeling procedure, there is no need for purification following the simple one-step, room-temperature incubation. The labeled protein is ready to use immediately, and any possible background from the unbound dye is eliminated. Due to the unique conjugation mechanism, proteins labeled with Py-Dyes maintain their native charge and isoelectric point. Because of their unique features, Chromeo Py-Dyes have been used successfully in a number of “no-wash” applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies.
Chromeo™ P543is an amine-reactive pyrylium dye