CalciFluor™ Fluo-8H, AM

CalciFluor™Fluo-8H，AM的开发旨在改善细胞负荷和钙反应，同时保持便利的Fluo-3和Fluo-4光谱波长，在〜490 nm处具有最大激发，在〜520 nm处具有最大发射。与Fluo 4 AM相似，阴离子羧基被乙酰氧基甲基酯保护以允许膜渗透，随后被细胞内酯酶去除-允许检测Ca2 +。但是，CalciFluor™Fluo-8，AM在室温下可以有效地加载到细胞中（而Fluo-3和Fluo-4需要更苛刻的加载条件），而CalciFluor™Fluo-8的亮度是Fluo-4的2倍和4倍比Fluo-

CalciFluor™ Fluo-8H, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases – allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8 is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior|in vivo|calcium indicator.
CalciFluor™ Fluo-8H, AMis a fluorescent calcium indicator with improved capability over Fluo-4 with nearly identical properties