ZnAF-2四盐酸盐
基本信息
| 产品名称 | ZnAF-2四盐酸盐 |
|---|---|
| 英文名称 | ZnAF-2 tetrahydrochloride |
| 别名 | 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein Tetrahydrochloride |
| 英文别名 | 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein Tetrahydrochloride |
| 规格或纯度 | ~5mM in 0.28mL in DMSO |
| 运输条件 | 超低温冰袋运输 |
一般描述
ZnAF-2是一种不可透过膜的乙二氨基荧光素化合物，可作为高亲和力Zn 2+特异性荧光探针（K d = 2.7 nM）。它具有低的基础荧光，当化学计量比（1：1）与Zn 2+结合时，荧光强度增加约51倍。ZnAF-2四盐酸盐对Ca 2+，Na +，Mg 2+或K +几乎没有亲和力。ZnAF-2已成功用于监测大鼠海马样本中细胞外Zn 2+释放的时空动态。ZnAF-2的可透过细胞的二乙酰化形式也可用于细胞内。
ZnAF-2 is a membrane-impermeable ethylenediaminofluoresceinyl compound that acts as a high-affinity Zn|2+|-specific fluorescent probe (K|d|= 2.7 nM). It has a low basal fluorescence which increases in intensity ~51-fold upon stoichiometric (1:1) binding to Zn|2+|. ZnAF-2 tetrahydrochloride exhibits little affinity towards Ca|2+|, Na|+|, Mg|2+|, or K|+|. ZnAF-2 has been used successfully in monitoring spatio-temporal dynamics of extracellular Zn|2+|release in rat hippocampal samples. A cell-permeable, diacetylated form of ZnAF-2 (sc-216074) is also available for intracellular use.
ZnAF-2 tetrahydrochlorideis a high-affinity Zn2+-specific fluorescent probe
相关属性
| 折光率 | n20D1.74 (Predicted) |
|---|---|
| 溶解性 | Soluble in DMSO (5 mM). |
| 储存温度 | -20°C储存 |
| 分子量 | 718.45 |
| 分子式 | C₃₄H₂₈N₄O₅・4HCl |
| 品牌 | Jinpan |
| Smiles | C1=CC=NC(=C1)CN(CCNC2=CC(=C(C=C2)C(=O)O)C3=C4C=CC(=O)C=C4OC5=C3C=CC(=C5)O)CC6=CC=CC=N6.Cl.Cl.Cl.Cl |
| PubChem CID | 16218345 |