菊粉-FITC
基本信息
产品名称 | 菊粉-FITC |
---|---|
英文名称 | Inulin–FITC |
英文别名 | Fluorescein isothiocyanate–inulin |
规格或纯度 | 大丽花块茎 |
运输条件 | 冰袋运输 |
相关属性
储存温度 | 2-8°C储存 |
---|---|
MDL号 | MFCD00131093 |
品牌 | Jinpan |
产品名称 | 菊粉-FITC |
---|---|
英文名称 | Inulin–FITC |
英文别名 | Fluorescein isothiocyanate–inulin |
规格或纯度 | 大丽花块茎 |
运输条件 | 冰袋运输 |
储存温度 | 2-8°C储存 |
---|---|
MDL号 | MFCD00131093 |
品牌 | Jinpan |
产品名称 | A1F APTS 聚糖 |
---|---|
英文名称 | A1F APTS Glycan |
英文别名 | A1F N-linked oligosaccharide. α(2,6)/FA2G2S(6)1, G2FS1, FA2G2S1, F(6)A2G2S1, F(6)A2G(4)2S(6)1 |
规格或纯度 | >80% |
运输条件 | 超低温冰袋运输 |
Product description:
Sources: A1F glycan is found on many mammalian glycoproteins including IgG, gamma globulins, and many serum glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine thyroglobulin by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution. Contains ammonium salt to stabilise against desialylation.
Molecular Weight: 2521
Fluorescence: λex = 425 nm, λem = 503 nm.
Amount: 20 pmols
储存温度 | -20°C储存 |
---|---|
品牌 | Jinpan |
产品名称 | 异硫氰酸荧光素-葡聚糖 |
---|---|
英文名称 | Fluorescein isothiocyanate–dextran |
别名 | FITC-右旋糖酐 |
英文别名 | FITC–Dextran |
规格或纯度 | 平均摩尔重量500000 |
运输条件 | 冰袋运输 |
一般描述
右旋糖酐是一种无水葡萄糖聚合物。由约 95% 的 α-D-(166) 键组成。其余 (163) 连接可解释右旋糖酐的分支。分支长度的冲突数据表明平均分支长度小于 3 个葡萄糖单位。然而,其他研究表明存在超过 50 个葡萄糖单位的分支。已发现天然右旋糖酐分子量 (MW) 在 900 万 ~5 亿范围内。较低分子量的右旋糖酐将表现出略少的分支,并且分子量分布范围更窄。分子量大于 10,000 的右旋糖酐表现出高度支链状。随着分子量的增加,葡聚糖分子的对称性增强。分子量为2,000至10,
应用
FITC-右旋糖酐作为荧光通量示踪化合物用于心血管、微循环、灌注、细胞单层和细胞膜渗透性研究,支持血流、膜损伤、血管引流和肾脏消除等过程的测量。小FITC-右旋糖酐也用于测量内吞作用和细胞连接通透性等过程。500 kDa 荧光素-右旋糖酐可用作脑脊液 (CSF) 示踪剂。FITC-右旋糖酐-500 可用于研究内皮细胞或上皮细胞单层孔隙度、细胞层内顶端基底侧运动和细胞连接渗透性。 FITC-右旋糖酐广泛用于利用微荧光法的微循环和细胞渗透性研究。FITC-右旋糖酐已被用于研究植物细胞壁孔隙率和毛细管渗透性。已证实血浆蛋白不与 FITC-右旋糖酐结合。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。
制备说明
通常以25mg / ml或更高的浓度测试FITC右旋糖酐在水中的溶解度。溶液应避光保存。在体内,FITC-右旋糖酐可稳定 24 小时以上。
General Description
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
Application
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 500 kDa, may be used as a cerebral spinal fluid (CSF) tracer. FITC-dextran-500 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
CAS编号 | 60842-46-8 |
---|---|
溶解性 | H2O:可溶25mg/mL,澄清至微混浊,黄色至深橙色;偶联物:FITC偶联物 |
储存温度 | 2-8°C储存 |
分子式 | |
品牌 | Jinpan |
Excitation and Emision Ranges | 493 nm and 518 nm |
x_emission | 518 |
x_excitation | 493 |
产品名称 | 3-脱氧-2-酮基-6-磷酸gluconic酸锂盐 |
---|---|
英文名称 | 3-Deoxy-2-keto-6-phosphogluconic acid lithium salt |
英文别名 | 3-Deoxy-2-keto-D-gluconic acid 6-phosphate lithium salt, (4S,5R)-4,5-Dihydroxy-2-oxo-6-phosphonooxyhexanoic acid lithium salt, KDPG, 2-Dehydro-3-deoxy-6-phospho-D-gluconate lithium salt, 2-Keto-3-deoxy-6-phosphogluconate lithium salt, 3-Deox |
规格或纯度 | ≥95% (TLC) |
运输条件 | 超低温冰袋运输 |
CAS编号 | 27244-54-8 |
---|---|
储存温度 | -20°C储存 |
品牌 | Jinpan |
产品名称 | BioQuant Man8 Glycan |
---|---|
英文名称 | BioQuant Man8 Glycan |
规格或纯度 | > 90% |
Product description:
Monoisotopic mass: 1721.6 [M+H]+
The BioQuant quantitative Man8 glycan is a purified and quantified glycan standard which can be used as an internal standard and positive control for quantitative analysis (e.g. monosaccharide analysis).
The amount of BioQuant quantitative Man8 glycan to be dispensed per vial is determined by quantitative Nuclear Magnetic Resonance (qNMR) of the bulk glycan stock. Once dispensed the amount of glycan per vial is determined by monosaccharide analysis.
品牌 | Jinpan |
---|
产品名称 | NA2F APTS 聚糖 |
---|---|
英文名称 | NA2F APTS Glycan |
英文别名 | NA2F N-linked oligosaccharide, FA2G2, F(6)A2G2, G2F |
规格或纯度 | >80% |
运输条件 | 超低温冰袋运输 |
Product description:
Sources: NA2F glycan is found on several mammalian glycoproteins including IgG, gamma globulins, and many serum glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine thryroglobulin by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Molecular Weight: 2229
Fluorescence: λex = 425 nm, λem = 503 nm.
Amount: 20 pmols
储存温度 | -20°C储存 |
---|---|
品牌 | Jinpan |
产品名称 | 异硫氰酸荧光素-葡聚糖 |
---|---|
英文名称 | Fluorescein isothiocyanate–dextran |
别名 | FITC-右旋糖酐 |
英文别名 | FITC–Dextran |
规格或纯度 | 平均摩尔重量60000-76000 |
运输条件 | 冰袋运输 |
应用
FITC-葡聚糖在心血管、微循环、灌注、细胞单层和细胞膜通透性研究中用作荧光通量示踪化合物,可实现对血流、膜损伤、血管引流和肾脏清除等过程进行检测。 小FITC-葡聚糖也可用于检测内吞作用和细胞连接通透性等过程。FITC-葡聚糖70 kDa(FD-70)可用作模型化合物,用于开发将分子递送到原生质体中和研究从水凝胶和脂质体等结构释放药物的方法。FD-70可用于微血管和细胞单层的通透性研究。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。
Application
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. FITC-dextran 70 kDa (FD-70) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. FD-70 may be used in microvascular and cell monolayer permeability studies. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
CAS编号 | 60842-46-8 |
---|---|
溶解性 | H2O:25mg/mL,清澈至微混浊,橙黄色;偶联物:FITC偶联物 |
储存温度 | 2-8°C储存 |
分子式 | |
品牌 | Jinpan |
Excitation and Emision Ranges | 493 nm and 518 nm |
x_emission | 518 |
x_excitation | 493 |
产品名称 | 2-AB Chitotriose – Quantitative |
---|---|
英文名称 | 2-AB Chitotriose – Quantitative |
The 2-AB labeled chitotriose standard is a fluorescently tagged linear tri-N-acetylglucosamine quantitative standard. The standard has been accurately dispensed from a bulk solution the concentration of which has been determined by quantitative NMR.
The chitotriose quantatative standard is also available as an unlabeled glycan and 2-AA labeled.
Accurate Quantity The bulk chitotriose concentration value was used to determine the amount of sample to be dispensed per vial to obtain 100 pmol of chitotriose per vial. Once dispensed the amount of chitotriose per vial is determined by monosaccharide analysis. The dispensing error is predicted to be than less 5%.
Regulatory Submissions Support system suitability and drugs regulatory submission by demonstrating consistent and reproducible glycosylation levels
Reliable Quantification Method Follows established glycan analysis techniques. Provides data comparable to gold-standard glycoprofiling methods based on monosaccharide analysis
Quick and Easy Used in QC routine in-house to determine quantity of both bulk and dispensed glycans
Use as Internal or External Quantitative Standard The Chitotriose standard can be spiked directly into your glycan sample or run in parallel.
Use as an Internal Standard Our unlabeled chitotriose standard can be used as an internal standard to quantify glycans in your sample. To do this, add a known amount of the unlabeled chitotriose to your unlabeled glycan sample. The mixture is then fluorescently labeled, cleaned up and run on HPLC/UHPLC. The unknown quantity of the glycan can be inferred by comparison of the chitotriose and glycan peak areas. The advantage of this method is that any sample loss occurring during the labelling and clean up stages will be the same for the sample and the chitotriose, thereby removing this as a source of error. The results obtained when running this method compare favourably with monosaccharide testing.
Use as External Standards The 2-AB and 2-AA labeled standard can be used as external standards to quantify glycans; these standards can be run directly on the HPLC/UHPLC. Again, the quantity of glycans in your sample is inferred by comparison of the peak areas for chitotriose (known amount) and your sample. This method is useful when quantification of already labeled glycans is required. They are also useful when quantification of several glycan samples is required in the same run, as the labeled chitotriose standard can be used as external standard for all the samples.
HILIC UHPLC column chromatogram of 2-AB labeled chitotriose and NGA2F glycans.
品牌 | Jinpan |
---|
产品名称 | 4,6-O-亚乙基-α-D-葡萄糖 |
---|---|
英文名称 | 4,6-O-Ethylidene-α-D-glucose |
英文别名 | NSC 89726, 4,6-O-Ethylidene α-D-glucopyranose, Ethylidene glucose |
CAS编号 | 13224-99-2 |
---|---|
熔点 | 168-173 °C |
Reaxys-RN | 5733441 |
MDL号 | MFCD00006820 |
分子量 | 206.19 |
分子式 | C8H14O6 |
品牌 | Jinpan |
PubChem CID | 93075 |
产品名称 | FA2G1 APTS 聚糖 |
---|---|
英文名称 | FA2G1 APTS Glycan |
英文别名 | FA2G1 N-linked oligosaccharide, G1F, F(6)A2G, NA2G1F |
规格或纯度 | >80% |
运输条件 | 超低温冰袋运输 |
Product description:
Sources: FA2G1 glycan is found on several mammalian glycoproteins including asialo serum transferrin and fibrin. This product is typically purified from the oligosaccharide pool released from bovine serum by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Molecular Weight: 2066
Fluorescence: λex = 425 nm, λem = 503 nm.
Amount: 20 pmols
储存温度 | -20°C储存 |
---|---|
品牌 | Jinpan |