鬼笔环肽CruzFluor™700共轭物

鬼笔环肽CruzFluor™700偶联物是近红外荧光偶联物，是AlexFluor®700的极佳替代品，与荧光素鬼笔环肽偶联物相比，保留了更高的光稳定性。鬼笔环肽是由死伞蘑菇伞形毒蕈产生的七肽鬼笔毒素。鬼笔环肽结合并稳定丝状F-肌动蛋白，防止成对的丝发生解离，并干扰细胞中丝状（聚合）肌动蛋白和球状（单体）肌动蛋白之间的自然平衡，并抑制F-肌动蛋白的ATP水解活性。肌动蛋白被细胞用于机械过程，例如生长，细胞骨架的支架和运动，并且这种肌动蛋白平衡的破坏破坏了细胞功能。肌动蛋白蛋白质在不同物种之间的差异不超过20

Phalloidin CruzFluor™ 700 Conjugate is a near infrared fluorescent conjugate that is an excellent substitute for AlexFluor® 700 and retains a higher photostabillity when compared to fluorescein phalloidin conjugates. Phalloidin is a heptapeptide phallotoxin produced by|Amanita phalloides|, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.|These properties of Phalloidin make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications.
Phalloidin CruzFluor™ conjugates can be used alongside antibodies in IF staining of formalin-fixed cells and formalin-fixed, frozen tissue sections. The Phalloidin will stain F-actin.1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMS
Phalloidin CruzFluor™ 700 Conjugateis a fluorescent phalloidin conjugate that selectively binds to F-actins