派洛宁Y
基本信息
| 产品名称 | 派洛宁Y |
|---|---|
| 英文名称 | Pyronin Y |
| 别名 | 3,6-双(二甲基氨基)呫吨鎓氯化物 |
| 英文别名 | Pyronin G;Pyronin J;3,6-Bis(dimethylamino)xanthylium chloride |
| 应用 | Used in Unna-Pappenheim stain to demonstrate RNA (red) in contrast to DNA (green). Also used for bacteria staining. |
| 规格或纯度 | biological stain |
| 运输条件 | 超低温冰袋运输 |
| 生化机理 | 嵌入RNA的阳离子染料，在电泳过程中显示为红色条带。显示在活细胞的线粒体中积累。在低浓度下，细胞生长抑制作用是将细胞停滞在G1期 |
一般描述
Pyronin Y is a dye that intercalates and stains RNA red, and has been shown to accumulate in the mitochondria of viable cells. Multidrμg-resistant (MDR) Acinetobacter baumannii studies tested inhibition of the efflux pump by measuring the intracellluar accumulation of Pyronin Y and Ethidium bromide. Research shows that at low Pyronin Y concentrations the cytostatic effect is cell arrest in the G1 phase, whereas at high concentrations the cells arrest is in the G2 and S phase. Pyronin Y has been used in combination with Alcian Blue to asses and study pulmonary diseases, and in combination with Methyl Green to stain nucleic acids in paraffin sections. Additionally Pyronin Y has been used to measure RNA in flow cytometry.Used in Unna-Pappenheim stain to demonstrate RNA (red) in contrast to DNA (green). Also used for bacteria staining.
Pyronin Y is a dye that intercalates and stains RNA red, and has been shown to accumulate in the mitochondria of viable cells. Multidrug-resistant (MDR) Acinetobacter baumannii studies tested inhibition of the efflux pump by measuring the intracellluar accumulation of Pyronin Y and Ethidium bromide. Research shows that at low Pyronin Y concentrations the cytostatic effect is cell arrest in the G1 phase, whereas at high concentrations the cells arrest is in the G2 and S phase. Pyronin Y has been used in combination with Alcian Blue to asses and study pulmonary diseases, and in combination with Methyl Green to stain nucleic acids in paraffin sections. Additionally Pyronin Y has been used to measure RNA in flow cytometry.
Used in Unna-Pappenheim stain to demonstrate RNA (red) in contrast to DNA (green). Also used for bacteria staining.
相关属性
| CAS编号 | 92-32-0 |
|---|---|
| 敏感性 | 对光线敏感 |
| 熔点 | 250-260°C |
| 溶解性 | Soluble in water (30 mg/mL), methyl cellosolve (9 mg/mL), and ethanol (3 mg/mL).DMSO : 25 mg/mL (82.56 mM; Need ultrasonic) |
| 储存温度 | -20°C储存 |
| MDL号 | MFCD00011725 |
| 分子量 | 302.81 |
| 分子式 | C17H19N2OCl |
| 品牌 | Jinpan |
| 备注 | 如果有可能，您尽量在同一天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。 |