产品简介
BCIP (5-Bromo-4-chloro-3-indolyl phosphate) 5-溴-4-氯-3-吲哚-磷酸盐＋NBT (四唑硝基蓝) 是碱性磷酸酶（AP）最佳的底物组合之一。在碱性磷酸酶的催化下，BCIP会被水解而产生强反应性的产物，该产物与NBT发生反应，形成不溶性的深蓝色至蓝紫色化合物。该试剂盒可用于AP系统的IHC 和Western Blot 实验的酶促显色。在AP催化下，在组织切片或印迹膜上结合了AP偶联物的地方产生深蓝色沉淀，可根据颜色反应来确定目的蛋白的位置及表达情况。
产品组分
40×BCIP：1 ml
40×NBT：1 ml
BCIP/NBT Buffer：40 ml
注意事项
1．工作液应现配现用，配制好的工作液1小时内有效。
2．工作液用量必需充足，保证完全覆盖组织片或印迹膜。
3．为获得最佳实验结果，请务必优化实验条件。
4．NBT有毒，使用时请采取必要的防护措施。
5．本产品仅用于科研，不能用于人体实验或人体治疗。
使用方法
1．BCIP/NBT显色工作液配制：
根据需要量，将40×BCIP、40×NBT和BCIP/NBT Buffer以1：1：38的体积比混匀后，即为BCIP/NBT显色工作液。
2．显色：
1）印迹膜显色：将配制好的工作液滴加在印迹膜上（或将印迹膜倾入到BCIP/NBT显色工作液中），室温避光孵育3-10分钟。显色完毕后，将膜浸入水中，终止反应。
2）组织切片或细胞爬片显色：滴加适量的BCIP/NBT显色工作液于需要显色的组织切片或细胞爬片上，室温避光孵育3-10分钟。显微镜下观察控制显色时间，当达到最佳显色效果后，自来水冲洗终止显色。显色后的切片经复染、脱水透明，封片后可长期保存。
Product Introduction
BCIP (5-Bromo-4-chloro-3-indolyl phosphate) 5-bromo-4-chloro-3-indolyl-phosphate + NBT (tetrazolium nitro blue) is the best substrate for alkaline phosphatase (AP) One of the combination. Under the catalysis of alkaline phosphatase, BCIP will be hydrolyzed to produce a highly reactive product, which reacts with NBT to form an insoluble dark blue to blue-violet compound. This kit can be used for the enzymatic color development of IHC and Western Blot experiments of the AP system. Under AP catalysis, a dark blue precipitate is produced where AP conjugates are combined on tissue sections or blotting membranes. The location and expression of the target protein can be determined based on the color reaction.
Product Components
40×BCIP: 1 ml
40×NBT: 1 ml
BCIP/NBT Buffer: 40 ml
Precautions
1. The working fluid should be prepared for immediate use, and the prepared working fluid will be effective within 1 hour.
2. The amount of working fluid must be sufficient to ensure complete coverage of the tissue sheet or blotting membrane. To
3. In order to obtain the best experimental results, be sure to optimize the experimental conditions.
4. NBT is poisonous, please take necessary protective measures when using it.
5. This product is only used for scientific research, not for human experiments or human treatment.
Instructions
1. BCIP/NBT color developing working solution preparation:
According to the required amount, mix 40×BCIP, 40×NBT and BCIP/NBT Buffer in a volume ratio of 1:1:38 to form the BCIP/NBT color developing working solution.
2. Color rendering:
1) Blotting membrane color development: Drop the prepared working solution on the blotting membrane (or pour the blotting membrane into the BCIP/NBT color developing working solution), and incubate for 3-10 minutes at room temperature and dark. After the color development is completed, the film is immersed in water to terminate the reaction.
2) Color development of tissue sections or cell slides: Drop an appropriate amount of BCIP/NBT color developing working solution on the tissue sections or cell slides that need color development, and incubate at room temperature for 3-10 minutes in the dark. Observe under the microscope to control the color development time. When the best color development effect is reached, rinse with tap water to stop the color development. After color development, the slices are counter-stained, dehydrated and transparent, and can be stored for a long time after mounting.
