L-组氨酸 7-酰氨基-4-甲基香豆素

L-组氨酸 7-酰氨基-4-甲基香豆素是酶的底物。糖苷酶，磷酸酶和酯酶的底物通常用于微生物学，食品/水，环境和ELISA测试中。肽酶的测定在临床酶测定中更为重要，AMC是大多数此类底物中使用的荧光团。AMC具有多种用途，因为任何氨基酸或肽的羧基末端均可与其连接。大多数酶对AMC结构具有耐受性。通常，酶反应动力学保持不变，几乎没有抑制作用。由于氨基的碱性较低，因此AMC在生理pH值下不受酸碱平衡的影响。因此，使用AMC底物的测定法对pH的耐受性较高。

L-Histidine 7-amido-4-methylcoumarin is an enzyme substrate. Substrates of glycosidases, phosphatases and esterases are commonly employed in microbiology, food/water, environmental and ELISA testing. Assays of peptidases are more important in clinical enzyme assays and AMC is the fluorophore employed in most of these substrates. AMC is extremely versatile since the carboxy-terminus of any amino acid or peptide can be linked to it. Most enzymes are tolerant towards the AMC structure. Typically, kinetics of enzyme reactions remain undisturbed and few inhibitory effects are observed. Due to the low basicity of the amino group, AMC is not subject to acid base equilibrium at physiological pH. Therefore, assays using AMC substrates are rather tolerant of pH.