异硫氰酸荧光素-葡聚糖

应用

用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

应用

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis. FITC-Dextran 250 (FD 250) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. It may be used as a model compound to study microvasculature and blood brain barrier. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis. FITC-Dextran 250 (FD 250) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. It may be used as a model compound to study microvasculature and blood brain barrier. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

应用

荧光素异硫氰酸酯-葡聚糖（FITC-葡聚糖70）可用作荧光探针以研究细胞过程，例如细胞渗透性、吞噬作用和胞吞作用，以及研究生物分子递送的机制。FITC-葡聚糖70可用于研究药物递送载体的包封和释放的容量、选择性和效率。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

其他说明

通过悬浮培养的植物细胞对FITC和FITC-葡聚糖进行内化；用于在白细胞中的液体胞饮作用的研究中

Application

Fluorescein isothiocyanate–dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-Dextran 70 may be used to study the capacity, selectivity and efficiency of encapsulation and release by drug delivery vehicles. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Other Notes

Internalisation of FITC and FITC-dextran by suspension cultured plant cells; In the study of fluid pinocytosis in leukocytes

异硫氰酸荧光素-葡聚糖

应用

异硫氰酸荧光素-葡聚糖（FITC-Dextran 2000）被用作荧光探针来研究结构的渗透性，例如参与听觉和血管渗透性过程的细胞间连接以及血脑屏障（BBB）的完整性等。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

Application

Fluorescein isothiocyanate–dextran (FITC–Dextran 2000) is use as a fluorescent probe to study the permeability of structures such as intercellular junctions involved in processes such as hearing and vascular permeability and the integrity of the blood brain barrier (BBB). Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

一般描述

右旋糖酐是一种无水葡萄糖的聚合物。 由大约95%的α-D-(166)键组成。 其余的 a (163) 键合解释了葡聚糖的分支。分支长度上的冲突数据意味着平均分支长度小于 3 个葡萄糖单位。但是，其他方法表明存在大于 50 个葡萄糖单位的分支。已发现天然右旋糖酐的分子量 (MW) 在 900 万至 500 万范围内。较低分子量的右旋糖酐将表现出略微较少的支化 ，并具有更窄的分子量分布范围。分子量大于10,000 的葡聚糖似乎是表现为高度支化的。 随着分子量的增加，葡聚糖分子

应用

FITC-葡聚糖被广泛用于利用微荧光法对微循环和细胞渗透性的研究。 FITC-葡聚糖已用于研究植物细胞壁的孔隙率和毛细管渗透性。血浆蛋白已被显示不可与FITC-葡聚糖进行结合。 FITC-葡聚糖已用于心血管、微循环、灌注细胞单层和细胞膜通透性研究，作为可支持对血流、膜损伤、血管引流和肾脏消除等过程进行测量的荧光通量示踪剂化合物。 小的FITC-葡聚糖已用于对内吞作用和细胞连接渗透性等过程进行测量。 5kDa的FITC-葡聚糖可用于对微尺度扩散测量进行校准。 5kDa的FITC-葡聚糖可用于在肠屏障功能障碍研究中对肠道通透性进行测量。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

通常在水中以25 mg/ml或更低的浓度对FITC葡聚糖的溶解度进行检测。 溶液应当避光保存。 FITC-葡聚糖在体外可稳定超过24小时。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. 5kDa FITC-dextran may be used to calibrate microscale diffusion measurements. 5kDa FITC-dextran may be used to measure gut permeability in gut barrier dysfunction research. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

异硫氰酸荧光素-葡聚糖

一般描述

葡聚糖是葡萄糖酐聚合物。 由大约95%的α-D-(166)键组成。 剩余的(163)键是葡聚糖的分支。分支长度的冲突数据意味着平均分支长度小于三个葡萄糖单位。但是，其他方法表明存在大于 50 个葡萄糖单位的分支。已发现的天然葡聚糖分子量（MW）在900万至5亿范围内。MW较低的葡聚糖分支略少，MW分布范围较窄。MW大于10,000 的葡聚糖表现得似乎它们是高度支化的。 随着MW的增加，葡聚糖分子的对称性增强。 MW为2,000至10,000的葡聚糖表现出可膨胀线圈的特性。

应用

FITC-葡聚糖被广泛用于运用显微荧光法进行微循环和细胞通透性研究。 FITC-葡聚糖被用于植物细胞壁孔隙度和毛细血管通透性研究。研究表明，血浆蛋白不与FITC-葡聚糖结合。 荧光素-葡聚糖，2000 kDa，用于使用小型集成荧光显微镜对活跃小鼠的脑微血管系统进行 体内 成像。FITC-葡聚糖，2000 kDa（FD-2000）在心血管、微循环、灌注和细胞膜通透性研究中用作荧光通量示踪化合物，可实现对血流、膜损伤、血管引流和肾脏清除等过程进行检测。 荧光素-葡聚糖，2000 kDa，用于使用小型集成荧光显微镜对活跃小鼠的脑微血管系统进行体内成像。 FITC-葡聚糖被用于植物细胞壁孔隙度研究。 用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

制备说明

通常以25 mg/ml或更高的浓度测试FITC葡聚糖在水中的溶解度。 溶液应避光保存。 在体内，FITC-葡聚糖稳定24小时以上。

General Description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Fluorescein-dextran, 2000 kDa, was used for in vivo imaging of brain microvasculature in active mice using a minature integrated fluorecent microscope. FITC-dextran, 2000 kDa (FD-2000) is used in cardiovascular, microcirculation, perfusion and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Fluorescein-dextran, 2000 kDa, may be used for in vivo imaging of brain microvasculature in active mice using a miniature integrated fluorescent microscope. FITC-dextran has been used to study plant cell wall porosity. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.