标签归档：bioquant

BioQuant Monosaccharide Standard

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BioQuant Monosaccharide Standard

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BioQuant Monosaccharide Standard

品牌：Jinpan
BioQuant Monosaccharide Standard

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
B498798-10nmol 10nmol 期货 BioQuant Monosaccharide Standard  

基本信息

产品名称 BioQuant Monosaccharide Standard
英文名称 BioQuant Monosaccharide Standard
运输条件 超低温冰袋运输

一般描述

The monosaccharide standard is a quantitative standard comprised of a mixture NIST-F and USP traceable monosaccharides including glucosamine (GlcN), galactosamine (GalN), galactose (Gal), mannose (Man), glucose/dextrose (Glc) and fucose (Fuc).

Form: Dry. Dried by centrifugal evaporation from an aqueous solution.

Amount: 10 nmol each of 6 monosaccharide standards


HPLC Analysis of the Monosaccharide Standards
HPLC analysis of 2-AA labeled monosaccharides is a robust method for determining monosaccharide levels in your glycoprotein sample.
1. Each monosaccharide has a different fluorescence yield per nmol. A standard curve of monosaccharide concentration versus fluorescence needs to be determined.

2. Free dye is not removed from the samples after 2-AA labeling to ensure no monosaccharides are lost in a dye clean-up stage. Free dye elutes as a large off-scale peak at about 15 minutes and also several large peaks after 28 minutes.

3. N-acetylglucosamine and N-acetylgalactosamine are both de-N-acetylated during the acid hydrolysis step of this process to produce glucosamine and galactosamine respectively.

4. Glucosamine is subject to an approximate 4 % epimerisation resulting in mannosamine. Mannosamine elutes immediately before the galactosamine peak, with no baseline resolution.

5. Galactosamine also produces an epimer peak, typically smaller than 2 %. However this epimer peak often elutes with the free dye peak.

6. The use of xylose as an internal standard allows samples to be compared directly. This allows compensation for any pipetting/sample preparation errors that may have occurred during sample processing.

7. Sample stability – we recommend keeping labeled samples in the freezer at -20 °C if you intend to store them for longer than one week.

BioQuant Monosaccharide Standard

HPLC profile of 2-aminobenzoic acid (2-AA) labeled mono-mix. The peak between 2 to 3 min is free dye.


相关属性

储存温度 -20°C储存
品牌 Jinpan

Chitotriose – BioQuant

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Chitotriose – BioQuant

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Chitotriose - BioQuant

品牌：Jinpan
Chitotriose – BioQuant

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
C498802-1EA 1个 期货 Chitotriose - BioQuant  

基本信息

产品名称 Chitotriose – BioQuant
英文名称 Chitotriose – BioQuant

一般描述

Product description：

The chitotriose quantatative standard is a linear tri-N-acetylglucosamine glycan, available as an unlabeled glycan or labeled with 2-AB and 2-AA. The amount of chitotriose to be dispensed per vial has been determined by quantitative nuclear magnetic resonance (qNMR) of the bulk chitotriose stock.

Amount: 5 ± 0.30 nmol by qNMR

Accurate Quantity Determined by quantitative NMR
5nmols of the lyophilised standard supplied in a 0.5mL vial.

Chitotriose - BioQuant

Regulatory Submissions Support system suitability and drugs regulatory submission by demonstrating consistent and reproducible glycosylation levels
Reliable Quantification Method Follows established glycan analysis techniques. Provides data comparable to gold-standard glycoprofiling methods based on Monosaccharide analysis

Quick and Easy Used in QC routine in-house to determine quantity of both bulk and dispensed glycans

Use as Internal or External Quantitative Standard The Chitotriose standard can be spiked directly into your glycan sample or run in parallel.


Integrates Easily With Fluorescent Labeling Workflows Adds into your existing labeling workflow, without requiring any extra steps.

Use as an Internal Standard Our unlabeled chitotriose standard can be used as an internal standard to quantify glycans in your sample. To do this, add a known amount of the unlabeled chitotriose to your unlabeled glycan sample. The mixture is then fluorescently labeled, cleaned up and run on HPLC/UHPLC. The unknown quantity of the glycan can be inferred by comparison of the chitotriose and glycan peak areas. The advantage of this method is that any sample loss occurring during the labelling and clean up stages will be the same for the sample and the chitotriose, thereby removing this as a source of error. The results obtained when running this method compare favourably with monosaccharide testing.
Use as External Standards The 2-AB and 2-AA labeled standards can be used as external standards to quantify glycans; these standards can be run directly on the HPLC/UHPLC. Again, the quantity of glycans in your sample is inferred by comparison of the peak areas for chitotriose (known amount) and your sample. This method is useful when quantification of already labeled glycans is required. They are also useful when quantification of several glycan samples is required in the same run, as the labeled chitotriose standard can be used as external standard for all the samples.

BioQuant Chitotriose Workflow

Chitotriose - BioQuant

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品牌 Jinpan

BioQuant Man8 Glycan

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BioQuant Man8 Glycan

> 90%

有货

BioQuant Man8 Glycan

品牌：Jinpan
BioQuant Man8 Glycan

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
B498804-1EA 1个 期货 BioQuant Man8 Glycan  

基本信息

产品名称 BioQuant Man8 Glycan
英文名称 BioQuant Man8 Glycan
规格或纯度 > 90%

一般描述

Product description：

Monoisotopic mass: 1721.6 [M+H]+
The BioQuant quantitative Man8 glycan is a purified and quantified glycan standard which can be used as an internal standard and positive control for quantitative analysis (e.g. monosaccharide analysis).
The amount of BioQuant quantitative Man8 glycan to be dispensed per vial is determined by quantitative Nuclear Magnetic Resonance (qNMR) of the bulk glycan stock. Once dispensed the amount of glycan per vial is determined by monosaccharide analysis.

Man8 Glycan Structure

BioQuant Man8 Glycan

HILIC UHPLC Analysis of Quantitative Man8 Glycan

BioQuant Man8 Glycan

HILIC UHPLC profile of procainamide labeled B498804

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品牌 Jinpan

BioQuant Xylose

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BioQuant Xylose

有货

BioQuant Xylose

品牌：Jinpan
BioQuant Xylose

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
B498815-100nmol 100nmol 期货 BioQuant Xylose  

基本信息

产品名称 BioQuant Xylose
英文名称 BioQuant Xylose
运输条件 超低温冰袋运输

一般描述

Product description：

Xylose can be used as an internal standard while performing monosaccharide analysis. This allows compensation for any pipetting/sample preparation errors that may have occurred during sample processing. Xylose is a monosaccharide typically found in plants and insects but not mammalian or yeast expression vectors. It should be added to each sample prior to labeling of released monosaccharides with 2-AA.
We recommend the use of the Monomix standard along with the Xylose reference standard when performing monosaccharide analysis. The Monomix monosaccharide standard enables instrument calibration in order to quantitatively determine the monosaccharide components in your glycoprotein.


Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Amount: 100 nmol

HPLC Analysis of the Monomix Standards with Xylose

HPLC analysis of 2-AA labeled monosaccharides is a robust method for determining monosaccharide levels in your glycoprotein sample. There are some important issues that need to be understood to enable effective data interpretation.

1. Each monosaccharide has a different fluorescence yield per nmol. A standard curve of monosaccharide concentration versus fluorescence needs to be determined.

2. Free dye is not removed from the samples after 2-AA labeling to ensure no monosaccharides are lost in a dye clean-up stage. Free dye elutes as a large off-scale peak at about 15 minutes and also several large peaks after 28 minutes.

3. N-acetylglucosamine and N-acetylgalactosamine are both de-N-acetylated during the acid hydrolysis step of this process to produce glucosamine and galactosamine respectively.

4. Glucosamine is subject to an approximate 4 % epimerisation resulting in mannosamine (Figure 2). Mannosamine elutes immediately before the galactosamine peak, with no baseline resolution.

5. Galactosamine also produces an epimer peak, typically smaller than 2 %. However this epimer peak often elutes with the free dye peak.

6. The use of xylose as an internal standard allows samples to be compared directly. This allows compensation for any pipetting/sample preparation errors that may have occurred during sample processing.

7. Sample stability – we recommend keeping labeled samples in the freezer at -20 °C if you intend to store them for longer than one week.

BioQuant Xylose

2AA-labeled monosaccharide standards profiled on UHPLC column.Peaks for the following monosaccharides appear within 8 minutes; glucosamine (GlcN), galactosamine (GalN), galactose (Gal), mannose (Man), glucose (Glc), xylose (Xyl) and fucose (Fuc).

相关属性

储存温度 -20°C储存
品牌 Jinpan