标签归档：buffer

Buffer Set (RNeasy 96 BioRobot 9604 Kit) 货号：1019992

发表于由

Buffer Set (RNeasy 96 BioRobot 9604 Kit) 货号：1019992

Buffer Set (RNeasy 96 BioRobot 9604 Kit)

货　　号：1019992

产品规格：96t

原  产  地：德国

参考价格：5330 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Buffer Set (RNeasy 96 BioRobot 9604 Kit)

1019992

制备DNA文库用于NGS应用

  • 快速的单管式操作可节约多达50%的时间
  • 从少量起始材料中获得高产量
  • 可选的高保真扩增步骤可进行无偏差扩增
  • 高通量多重扩增
GeneRead Library Prep Kits可为NGS应用制备文库，工作流程经过优化，十分高效。该试剂盒能够快速、高效的制备DNA文库，制备的文库可用于Illumina或Life Technologies的Ion Torrent NGS平台。

原理
GeneRead Library Prep Kits的单管式工作流程经过优化，能够稳定获得高产量DNA文库；文库适合用于Illumina或Life Technologies的Ion Torrent NGS平台。与其他供应商的文库制备工作流程相比，快速的单管式实验流程采取较少的回收步骤，且包含可选的高保真文库扩增步骤。GeneRead Library Prep Kits极大程度减少了手工操作可能带来的污染和失误风险，节省时间和人力（参见Optimized workflow for Illumina-compatible DNA libraries和Optimized workflow for Ion Torrent-compatible DNA libraries）。

操作流程
制备适用于Illumina平台的DNA文库的实验流程

由长片段DNA组成的样本首先被剪切为300 bp（使用Illumina MiSeq仪器，version 1）或500 bp（使用Illumina MiSeq仪器，version 2）的中等长度片段的随机文库。剪切后，修复DNA片段两端，在每条链的3’端加上Poly A尾巴。之后，将扩增和测序所必须的接头连接到DNA片段两端。GeneRead Library Prep (I) Kit中含有的Barcode接头含有独特的辨别序列，因此能够进行多重测序反应。之后选择一定长度的片段进行纯化。可使用GeneRead Size Selection Kit（货号180514）进行准确的片段回收和片段长度选择（参见Precise size selection）。为确保从少量起始材料中获得高产量，可选择性进行高保真扩增步骤。

制备适用于Ion Torrent平台的DNA文库的实验流程

由长片段DNA组成的样本首先被剪切为400 bp（使用Ion Torrent PGM仪器）或200 bp（使用Ion Proton仪器）的中等长度片段的随机文库。剪切后，修复DNA片段两端。之后，将扩增和测序所必须的接头连接到DNA片段两端。GeneRead Library Prep (L) Kit中含有的Barcode接头含有独特的辨别序列，因此能够进行多重测序反应。之后选择一定长度的片段进行纯化。为确保从少量起始材料中获得高产量， 可选择性进行高保真扩增步骤。

应用
GeneRead Library Prep Kits能够快速、高效的制备DNA文库，制备的文库可用于Illumina或Life Technologies的Ion Torrent NGS平台。

产品详细信息

Buffer Set (RNeasy 96 BioRobot 9604 Kit)

1019992

制备DNA文库用于NGS应用

  • 快速的单管式操作可节约多达50%的时间
  • 从少量起始材料中获得高产量
  • 可选的高保真扩增步骤可进行无偏差扩增
  • 高通量多重扩增
GeneRead Library Prep Kits可为NGS应用制备文库，工作流程经过优化，十分高效。该试剂盒能够快速、高效的制备DNA文库，制备的文库可用于Illumina或Life Technologies的Ion Torrent NGS平台。

原理
GeneRead Library Prep Kits的单管式工作流程经过优化，能够稳定获得高产量DNA文库；文库适合用于Illumina或Life Technologies的Ion Torrent NGS平台。与其他供应商的文库制备工作流程相比，快速的单管式实验流程采取较少的回收步骤，且包含可选的高保真文库扩增步骤。GeneRead Library Prep Kits极大程度减少了手工操作可能带来的污染和失误风险，节省时间和人力（参见Optimized workflow for Illumina-compatible DNA libraries和Optimized workflow for Ion Torrent-compatible DNA libraries）。

操作流程
制备适用于Illumina平台的DNA文库的实验流程

由长片段DNA组成的样本首先被剪切为300 bp（使用Illumina MiSeq仪器，version 1）或500 bp（使用Illumina MiSeq仪器，version 2）的中等长度片段的随机文库。剪切后，修复DNA片段两端，在每条链的3’端加上Poly A尾巴。之后，将扩增和测序所必须的接头连接到DNA片段两端。GeneRead Library Prep (I) Kit中含有的Barcode接头含有独特的辨别序列，因此能够进行多重测序反应。之后选择一定长度的片段进行纯化。可使用GeneRead Size Selection Kit（货号180514）进行准确的片段回收和片段长度选择（参见Precise size selection）。为确保从少量起始材料中获得高产量，可选择性进行高保真扩增步骤。

制备适用于Ion Torrent平台的DNA文库的实验流程

由长片段DNA组成的样本首先被剪切为400 bp（使用Ion Torrent PGM仪器）或200 bp（使用Ion Proton仪器）的中等长度片段的随机文库。剪切后，修复DNA片段两端。之后，将扩增和测序所必须的接头连接到DNA片段两端。GeneRead Library Prep (L) Kit中含有的Barcode接头含有独特的辨别序列，因此能够进行多重测序反应。之后选择一定长度的片段进行纯化。为确保从少量起始材料中获得高产量， 可选择性进行高保真扩增步骤。

应用
GeneRead Library Prep Kits能够快速、高效的制备DNA文库，制备的文库可用于Illumina或Life Technologies的Ion Torrent NGS平台。

Flow Cytometry Staining Buffer 货号：00-4222-57

发表于由

Flow Cytometry Staining Buffer 货号：00-4222-57

Flow Cytometry Staining Buffer

货　　号：00-4222-57

产品规格：200ml

原  产  地：USA

参考价格：588 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Flow Cytometry Staining Buffer

00-4222-57

200ml

USA

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set in combination with BrdU and DNase I steps allows for detection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newlysynthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, thecells are stained for BrdU incorporation along with any other cell surface and/orintracellular targets of interest.

 

产品详细信息

Flow Cytometry Staining Buffer

00-4222-57

200ml

USA

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set in combination with BrdU and DNase I steps allows for detection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newlysynthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, thecells are stained for BrdU incorporation along with any other cell surface and/orintracellular targets of interest.

 

1X RBC Lysis Buffer 货号：00-4333-57

发表于由

1X RBC Lysis Buffer 货号：00-4333-57

1X RBC Lysis Buffer

货　　号：00-4333-57

产品规格：200ml

原  产  地：USA

参考价格：552 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

 1X RBC Lysis Buffer

00-4333-57
200ml

USA

The eBioscience 1X Red Blood Cell Lysis Buffer is formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood.

This buffer contains ammonium chloride, which lyses red cells with minimal effect on lymphocytes when used as instructed. Nucleated red cells are not effectively lysed with ammonium chloride. RBC lysis is not necessary when working with mouse thymus and lymph nodes

产品详细信息

 1X RBC Lysis Buffer

00-4333-57
200ml

USA

The eBioscience 1X Red Blood Cell Lysis Buffer is formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood.

This buffer contains ammonium chloride, which lyses red cells with minimal effect on lymphocytes when used as instructed. Nucleated red cells are not effectively lysed with ammonium chloride. RBC lysis is not necessary when working with mouse thymus and lymph nodes

Flow Cytometry Staining Buffer 货号：00-4222-57

发表于由

Flow Cytometry Staining Buffer 货号：00-4222-57

Flow Cytometry Staining Buffer

货　　号：00-4222-57

产品规格：200ml

原  产  地：USA

参考价格：588 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Flow Cytometry Staining Buffer

 00-4222-57
200ml

USA

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set in combination with BrdU and DNase I steps allows for detection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newlysynthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, thecells are stained

for BrdU incorporation along with any other cell surface and/orintracellular targets of interest.

产品详细信息

Flow Cytometry Staining Buffer

 00-4222-57
200ml

USA

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set in combination with BrdU and DNase I steps allows for detection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newlysynthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, thecells are stained

for BrdU incorporation along with any other cell surface and/orintracellular targets of interest.

BrdU Staining Buffer Set for Flow Cytometry Kit 货号：00-5525-00

发表于由

BrdU Staining Buffer Set for Flow Cytometry Kit 货号：00-5525-00

BrdU Staining Buffer Set for Flow Cytometry Kit

货　　号：00-5525-00

产品规格： KIT

原  产  地：USA

参考价格：1788 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

 BrdU Staining Buffer Set for Flow Cytometry Kit

00-5525-00
    KIT
USA

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis.

This set in combination with BrdU and DNase I steps allows fordetection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomicDNA during the S-phase of mitosis. Following DNA denaturation, the cells arestained for
BrdU incorporation along with any other cell surface and/orintracellular targets of interest.

产品详细信息

 BrdU Staining Buffer Set for Flow Cytometry Kit

00-5525-00
    KIT
USA

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis.

This set in combination with BrdU and DNase I steps allows fordetection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomicDNA during the S-phase of mitosis. Following DNA denaturation, the cells arestained for
BrdU incorporation along with any other cell surface and/orintracellular targets of interest.

Permeabilization Buffer (10X) 货号：00-8333-56

发表于由

Permeabilization Buffer (10X) 货号：00-8333-56

Permeabilization Buffer (10X)

货　　号：00-8333-56

产品规格： 100ml

原  产  地：USA

参考价格：1020 （参考价格，以实际价格为准）

优惠价格：

产品详细信息
eBioscience Permeabilization Buffer, in conjunction with eBioscience Intracellular (IC) Fixation Buffer (Cat. No. 00-8222) or Foxp3
buffers (Cat. No. 00-5521) can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmicand/or nuclear antigens. These solutions have been specially formulated for reducing non-specific staining of fluorochrome labelled antibodies and increasing specific fluorescence signal to noise ratios.
The Fixation Buffer is used first followed by Permeabilization Buffer. Subsequent washing steps, antibody dilutions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer. The finalwashing step and cell resuspension prior to running the sample is done using FlowCytometry Staining Buffer.
The Permeabilization Buffer is supplied as a 10X concentrate. The 10X stock should be diluted in distilled water to a 1X solution prior to use. The 1X final working solution contains 0.1% saponin and 0.009% sodium azide.

产品详细信息 eBioscience Permeabilization Buffer, in conjunction with eBioscience Intracellular (IC) Fixation Buffer (Cat. No. 00-8222) or Foxp3
buffers (Cat. No. 00-5521) can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmicand/or nuclear antigens. These solutions have been specially formulated for reducing non-specific staining of fluorochrome labelled antibodies and increasing specific fluorescence signal to noise ratios.
The Fixation Buffer is used first followed by Permeabilization Buffer. Subsequent washing steps, antibody dilutions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer. The finalwashing step and cell resuspension prior to running the sample is done using FlowCytometry Staining Buffer.
The Permeabilization Buffer is supplied as a 10X concentrate. The 10X stock should be diluted in distilled water to a 1X solution prior to use. The 1X final working solution contains 0.1% saponin and 0.009% sodium azide.

IC Fixation Buffer 货号：00-8222-49

发表于由

IC Fixation Buffer 货号：00-8222-49

IC Fixation Buffer

货　　号：00-8222-49

产品规格： 125ml

原  产  地：USA

参考价格：1020 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

 IC Fixation Buffer

00-8222-49

125ml
USA

eBioscience Intracellular (IC) Fixation Buffer can be used to fix

cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333),
when performing intracellular staining of antigens located in thecytoplasm and
secretory pathway, such as cytokines, chemokines and growth factors.This buffer,
when used as instructed, is compatible with surface stainingfluorochrome conjugated
antibodies and can be used as a short term storage buffer for
fluorochrome (including tandem dyes)-stained cells for up to 3 days at2-8oC (stored in the dark).

产品详细信息

 IC Fixation Buffer

00-8222-49

125ml
USA

eBioscience Intracellular (IC) Fixation Buffer can be used to fix

cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333),
when performing intracellular staining of antigens located in thecytoplasm and
secretory pathway, such as cytokines, chemokines and growth factors.This buffer,
when used as instructed, is compatible with surface stainingfluorochrome conjugated
antibodies and can be used as a short term storage buffer for
fluorochrome (including tandem dyes)-stained cells for up to 3 days at2-8oC (stored in the dark).