FITC-CM-葡聚糖

应用

FITC-CM-葡聚糖40，一种阴离子荧光葡聚糖，可用于研究细胞过程，如细胞渗透性、吞噬作用和内吞作用，以及生物分子传递的机制。FITC-CM-葡聚糖40可用于比较其选择性和中性FITC-葡聚糖40。

Description

FITC-CM-Dextran 40, an anionic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-CM-Dextran 40 may be use to compare its selectivity versus neutral FITC-Dextran 40.

FITC-CM-葡聚糖

FITC-CM-葡聚糖

应用

FITC-CM-Dextran 70 是一种阴离子荧光葡聚糖，可用于研究细胞的通透性、吞噬作用和内吞作用等细胞过程，以及研究生物分子的传递机制。FITC-CM-Dextran 70 可用于比较其与中性 FITC-Dextran 70 的选择性。

Description

FITC-CM-Dextran 70, an anionic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-CM-Dextran 70 may be use to compare its selectivity versus neutral FITC-Dextran 70.

FITC-CM-葡聚糖

应用

FITC-CM-葡聚糖4，一种阴离子荧光葡聚糖，可用于研究细胞过程，如细胞渗透性、吞噬作用和内吞作用，以及生物分子传递的机制。FITC-CM-葡聚糖4可用于比较其选择性和中性FITC-葡聚糖4。

Description

FITC-CM-Dextran 4, an anionic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-CM-Dextran 4 may be use to compare its selectivity versus neutral FITC-Dextran 4.

FITC-CM-聚蔗糖

FITC-CM-polysucroses are standard FITC-polysucrose carrying carboxymethyl-substituents. Polysucrose (renamed from Ficoll™) is a polymer which displays excellent biocompatibility. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. Aladdin produce FITC-CM-polysucroses with a molecular weight of 70 kDa and 400 kDa. FITC-CM-polysucrose is supplied as a yellow powder.

Structure

Polysucrose is a polymer synthesised by cross-linking sucrose with epichlorohydrin. FITC-CM-polysucroses are manufactured by reacting selected polysucrose fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the polysucrose chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. Polysucrose has a more spherical structure and is less flexible compared to dextran.

Spectral data

FITC-polysucrose has an excitation maximum of 495 and an emission maximum at 517 nm at pH 9.

Application

FITC-CM-polysucrose are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. FITC-CM-polysucrose has played an interesting role in elucidating the properties of the glomerular membrane.

FITC-CM-polysucroses are standard FITC-polysucrose carrying carboxymethyl-substituents. Polysucrose (renamed from Ficoll™) is a polymer which displays excellent biocompatibility. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. Aladdin produce FITC-CM-polysucroses with a molecular weight of 70 kDa and 400 kDa. FITC-CM-polysucrose is supplied as a yellow powder.

Structure

Polysucrose is a polymer synthesised by cross-linking sucrose with epichlorohydrin. FITC-CM-polysucroses are manufactured by reacting selected polysucrose fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the polysucrose chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. Polysucrose has a more spherical structure and is less flexible compared to dextran.

Spectral data

FITC-polysucrose has an excitation maximum of 495 and an emission maximum at 517 nm at pH 9.

Application

FITC-CM-polysucrose are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. FITC-CM-polysucrose has played an interesting role in elucidating the properties of the glomerular membrane.