Hotstart HiTaq One-Step RT-PCR Mix
基本信息
| 产品名称 | Hotstart HiTaq One-Step RT-PCR Mix |
|---|---|
| 英文名称 | Hotstart HiTaq One-Step RT-PCR Mix |
| 运输条件 | 超低温冰袋运输 |
一般描述
产品简介
One-Step RT-PCR Mix 以其卓越的快速性及定量性被广泛应用，使用该产品进行 Real Time RT-PCR 反应可在同一反应管内连续进行，操作简单，并能有效防止污染。本制品中使用了最适合于 Real Time RT-PCR 的超级反转录酶（Super MMLV Reverse Transcriptase）和化学修饰热启动酶（Hotstart HiTaq DNA Polymerase），具有高扩增效率和高扩增特异性，能进行稳定的 Real Time One Step RT-PCR 反应， 大大提高了检测灵敏度，并省略了PCR 反应后的电泳步骤，非常适合于 RNA 病毒等微量 RNA 的检测。
本制品专为进行便利、高灵敏度的一步式 RT-PCR 设计。其独特的酶和特别设计的缓冲液确保了逆转录和PCR 反应高效、准确的进行，无需进行优化。可以在宽广的定量区域内得到良好的标准曲线，对靶基因进行准确定量检测，重复性好，可信度高。
产品特点
快速方便的单管反应；
适合各种 RNA 模板，无需优化反应条件；
独特的酶组合，确保反应的高特异性和高灵敏度；
经优化的缓冲体系确保了高效的逆转录和扩增过程。
产品内容
Hotstart HiTaq DNA Polymerase ( 5U/μl )
Super MMLV Reverse Transcriptase (200U/μl )
RNasin ( 40U/μl )
5×HS HiTaq One Step RT-PCR Buffer(Mg2+ Plus)
Solution I ( 10× )
dNTP Mix ( 25mM )
使用方法
1. 体系配制（50μl 体系）
|
组分 |
体积 |
终浓度 |
|
Hotstart HiTaq DNA Polymerase |
0.75μl |
— |
|
Super MMLV Reverse Transcriptase |
0.4μl |
— |
|
RNasin |
0.5μl |
— |
|
5×HS HiTaq One Step RT-PCR Buffer |
10μl |
1× |
|
Solution I ( 10× ) |
5μl |
1× |
|
dNTP Mix |
0.4μl |
0.2mM |
|
Primer-probe Mix |
3μl ※ |
— |
|
模板 |
5μl ※ |
— |
|
超纯水 |
Up to 50μl |
— |
|
总体积 |
50μl |
— |
※ 注：引物、探针以及模板的用量可自行调整，体系不够请用超纯水补齐。
2. 反应条件
|
步骤 |
温度 |
时间 |
循环数 |
|
1 |
50℃ |
15min |
1 |
|
2 |
95℃ |
10min |
1 |
|
3 |
94℃ |
15s |
40※ |
|
4 |
55℃ |
40s（收集荧光） |
※注：反应条件根据引物探针和模板情况可自行设置。
Product Introduction
One-Step RT-PCR Mix is widely used due to its excellent rapidity and quantification. Real Time RT-PCR reactions can be carried out continuously in the same reaction tube with this product, which is simple to operate and can effectively prevent contamination. This product uses Super MMLV Reverse Transcriptase (Super MMLV Reverse Transcriptase) and chemically modified Hotstart HiTaq DNA Polymerase, which are most suitable for Real Time RT-PCR, with high amplification efficiency and high amplification specificity. It can perform a stable Real Time One Step RT-PCR reaction, greatly improving the detection sensitivity, and omitting the electrophoresis step after the PCR reaction, which is very suitable for the detection of RNA viruses and other trace RNAs.
This product is designed for convenient and highly sensitive one-step RT-PCR. Its unique enzymes and specially designed buffers ensure that reverse transcription and PCR reactions are carried out efficiently and accurately without optimization. A good standard curve can be obtained in a wide quantitative area, and the target gene can be accurately and quantitatively detected, with good repeatability and high reliability.
Features
Quick and convenient single tube reaction;
Suitable for various RNA templates, no need to optimize reaction conditions;
Unique enzyme combination to ensure high specificity and high sensitivity of the reaction;
The optimized buffer system ensures an efficient reverse transcription and amplification process.
Product content
Hotstart HiTaq DNA Polymerase ( 5U/μl )
Super MMLV Reverse Transcriptase (200U/μl )
RNasin ( 40U/μl )
5×HS HiTaq One Step RT-PCR Buffer(Mg2+ Plus)
Solution I ( 10× )
dNTP Mix ( 25mM )
Instructions
1. System preparation (50μl system)
|
Reagent |
Volume |
Final concentration |
|
Hotstart HiTaq DNA Polymerase |
0.75μl |
— |
|
Super MMLV Reverse Transcriptase |
0.4μl |
— |
|
RNasin |
0.5μl |
— |
|
5×HS HiTaq One Step RT-PCR Buffer |
10μl |
1× |
|
Solution I ( 10× ) |
5μl |
1× |
|
dNTP Mix |
0.4μl |
0.2mM |
|
Primer-probe Mix |
3μl ※ |
— |
|
Template |
5μl ※ |
— |
|
Ultra-pure water |
Up to 50μl |
— |
|
Total capacity |
50μl |
— |
※ Note: The amount of primers, probes and templates can be adjusted by yourself. If the system is not enough, please use ultrapure water to make up.
2.Reaction conditions
|
Step |
Temperature |
Time |
Number of cycles |
|
1 |
50℃ |
15min |
1 |
|
2 |
95℃ |
10min |
1 |
|
3 |
94℃ |
15s |
40※ |
|
4 |
55℃ |
40s（Collect fluorescence） |
※Note: The reaction conditions can be set according to the primer probe and template.
相关属性
| 储存温度 | -20°C储存 |
|---|---|
| 品牌 | Jinpan |