qiagen试剂盒 货号:

qiagen试剂盒 货号:

qiagen试剂盒

货  号:

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参考价格: (参考价格,以实际价格为准)

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产品详细信息

EZ1 DNA Blood 350 µl Kit (48)               951054

miRNeasy FFPE Kit (50)                     217504

Qproteome Albumin/IgG Depletion Kit    37521

QIAGEN Multiplex PCR Plus Kit (30)             206151

QIAquick Nucleotide Removal Kit (250)          28306

QIAGEN OneStep RT-PCR Kit (100)              210212

HotStarTaq Master Mix Kit (1000)                  203445

miScript SYBR Green PCR Kit (200)                 218073

GeneRead DNA Library I Core Kit (12)               180432

mericon Ruminant Kit (24)                                292073

mericon Vibrio triple Kit (24)                             290133

PyroMark Q24 Advanced Kit (4×24)                  970902

PAXgene TissueFIX Cont. (50 ml) Kit              765312

Shucker Finger Kit                                       9237290

QIAGEN Multiplex PCR Kit (100)                      206143

Allprep FFPE DNA/ RNA Kit (50)                    80234

Buffer Set (RNeasy 96 BioRobot 9604 Kit)            1019992

GeneRead DNA Library I Amp Kit (100)                180455

GeneRead DNA Library L Amp Kit (100)                 180485

EpiTect Plus LyseAll Bisulfite Kit (48)                     59164

mericon Y. enterocolitica Kit (24)                           290113

mericon Salmonella spp Kit (24)                             290013

QuantiFast Probe RT-PCR PLUS Kit (80)                     204482

RNeasy Protect Cell Mini Kit (50)                          74624

EpiTect Plus DNA Bisulfite Kit (48)                              59124

Qproteome Plasma Membrane Protein Kit                    37601

Rotor-Gene Type-it HRM Discovery Kit                          206541

EZ1 DNA Blood 200 µl Kit (48)                                    951034

GeneRead Size Selection Kit (50)                                  180514

EpiTect Plus FFPE Bisulfite Kit (48)                                  59144

QuantiFast Probe RT-PCR PLUS Kit (400)                           204484

QuantiTect Virus +ROX Vial Kit (50)                                  211031

mericon Sheep Kit (24)                                                   292063

mericon L. monocytogenes Kit (24)                                    290023

miRNeasy Mini Kit (50)                                                       217004

QIAamp DSP Virus Spin Kit (50)                                          61704

mericon Screen nos Kit (24)                                             291043

mericon Cronobacter spp Kit (24)                                        290063

PAXgene Blood DNA Kit (25)                                             761133

RNeasy Plus Micro Kit (50)                                                  74034

QuantiTect Virus Kit (50)                                                     211011

MinElute 96 UF PCR Purification Kit (4)                                   28051

QuantiTect Multiplex PCR NoROX Kit (200)                           204743

PAXgene Blood DNA Kit (25)                                             761133

RNeasy Plus Micro Kit (50)                                                  74034

QuantiTect Virus Kit (50)                                                    211011

MinElute 96 UF PCR Purification Kit (4)                                      28051

mericon Campylobacter triple Kit (24)                                         290043

mericon Screen 35S Kit (24)                                                     291013

QuantiFast Probe RT-PCR+ROXvial Kit(400)                                  204554

GeneRead rRNA Depletion Kit (6)                                              180211

mericon Goat Kit (24)                                                               292053

Type-it Fast SNP Probe PCR Kit (100)                                            206042

MagAttract DNA Blood Mini M48 Kit (192)                                       951336

GeneRead Library Quant Kit                                                           180612

GeneRead DNA Library L Core Kit (12)                                            180462

DyeEx 2.0 Spin Kit (50)                                                                 63204

mericon DNA Bacteria Plus Kit (50)                                                    69534

Type-it HRM PCR Kit (400)                                                               206544

QuantiFast Pathogen PCR +IC Kit (100)                                             211352

QuantiFast Pathogen RT-PCR +IC Kit (100)                                         211452

DNeasy mericon Food Kit (50)                                                          69514

EZ1 DSP DNA BLD Card                                                                     37571

CompactPrep Plasmid Maxi Kit (25)                                                       12863

Effectene Transfection Reag. Kit (0.3ml)                                               1054250

Qproteome Nuclear Protein Kit                                                               37582

cador T. equigenitalis PCR Kit                                                                  285023

CompactPrep Plasmid Midi Kit (25)                                                            12843

mericon Soy Kit (24)                                                                              293013

mericon Shigella spp Kit (24)                                                                   290103

MagAttract Viral RNA M48 Kit (96)                                                          955235

MagAttract Viral RNA M48 Kit (96)                                                         955235

TopTaq Master Mix Kit (250)                                                                  200403

QuantiFast Multiplex PCR Kit (80)                                                             204652

Qproteome Mammalian Protein Prep Kit                                                    37901

BioSprint 96 DNA Plant Kit (576)                                                              941557

QIAamp cador Pathogen Mini Kit (50)                                                          54104

Type-it Microsatellite PCR Kit (200)                                                           206243

Type-it Mutation Detect PCR Kit (200)                                                       206343

Type-it Mutation Detect PCR Kit (70)                                                         206341

MagAttract HMW DNA Kit (48)                                                                 67563

Qproteome Murine Albumin Depletion Kit                                                     37591

Microbial DNA qPCR Assay Kits                                                                    330033

BioSprint 15 DNA Blood Kit (45)                                                                940014

miScript Plant RT Kit (50)                                                                          218761

QIAamp UCP Pathogen Mini Kit (50),G                                                           50214

PAXgene Tissue RNA Kit (50)                                                                    765134

QIAGEN Multiplex PCR Plus Kit (100)                                                             206152

QuantiTect Multiplex PCR Kit (200)                                                               204543

EpiTect MSP PCR Kit (100)                                                                             59305

QuantiTect Rev.Transcription Kit (50)                                                                205311

QuantiTect Rev.Transcription Kit (10)                                                                205310

QIAamp DNA FFPE Tissue Kit (50)                                                                    56404

PAXgene Tissue DNA Kit (50)                                                                          767134

QIAamp Fast DNA Stool Mini Kit (50)                                                                   51604

QuantiNova SYBR Green PCR Kit (500)                                                           208054

QuantiTect Probe PCR + UNG Kit (200)                                                          204363

Rotor-Gene Probe RT-PCR Kit (400)                                                               204574

QuantiTect Multiplex PCR NoROX Kit (40)                                                        204741

EpiTect Fast DNA Bisulfite Kit (50)                                                                  59824

Ni-NTA Spin Kit (50)                                                                                      31314

QIAprep Spin M13 Kit (50)                                                                             27704

Oligotex Direct mRNA Mini Kit                                                                          72022

QuantiNova SYBR Green PCR Kit (100)                                                             208052

QuantiTect Probe PCR Kit (40)                                                                       204341

NeXtal Stock Kit Cryo                                                                                      132981

QIAamp UCP PurePathogen Blood Kit (10)                                                          50112

QuickLyse Miniprep Kit (100)                                                                            27405

GeneRead DNA FFPE Kit (50)                                                                           180134

QIAvac Holder Kit                                                                                          19418

QuantiTect Multiplex PCR Kit (40)                                                                    204541

EpiTect Fast LyseAll Bisulfite Kit (50)                                                                   59864

miScript II RT Kit (12)                                                                                     218160

miScript PCR Starter Kit (80)                                                                                218193

Penta.His HRP Conjugate Kit                                                                                  34460

QIAquick PCR Purification Kit (50)                                                                            28104

QIAfilter Plasmid Maxi Kit (10)                                                                                   12262

Anti·His Antibody Selector Kit                                                                                  34698

QIAquick Gel Extraction Kit (50)                                                                            28704

MinElute PCR Purification Kit (50)                                                                          28004

QIAfilter Plasmid Midi Kit (25)                                                                              12243

QIAGEN Plasmid Mini Kit (25)                                                                              12123

QIAGEN PCR Cloning Kit (40)                                                                              231124

RNeasy Plant Mini Kit (20)                                                                                   74903

Ni-NTA Superflow Cartridges (5×1 ml)                                                                  30721

QIAquick Gel Extraction Kit (250)                                                                         28706

Blood & Cell Culture DNA Mini Kit                                                                           13323

QIAGEN Plasmid Mini Kit (100)                                                                              12125

EndoFree Plasmid Giga Kit (5)                                                                               12391

NeXtal Stock Kit Salt                                                                                            132985

QuickLyse Miniprep Kit (250)                                                                                  27406

QuantiTect SYBR Green PCR +UNG Kit (200)                                                         204163

QIAGEN Plasmid Maxi Kit (10)                                                                              12162

EndoFree Plasmid Maxi Kit (10)                                                                            12362

EpiTect Fast FFPE Bisulfite Kit (50)                                                                       59844

REPLI-g Human Control Kit                                                                                 150090

RNeasy Protect Animal Blood Kit (50)                                                                  1054578

QIAamp MinElute Media Kit (50)                                                                         57414

MinElute Gel Extraction Kit (50)                                                                          28604

Blood & Cell Culture DNA Midi Kit                                                                         13343

EndoFree Plasmid Mega Kit (5)                                                                           12381

PAXgene Bone Marrow RNA Kit (30)                                                                   764133

miScript II RT Kit (50)                                                                                       218161

Omniscript RT Kit (10)                                                                                     205110

QIAamp DNA Stool Mini Kit (50)                                                                           51504

QIAquick PCR Purification Kit (250)                                                                     28106

QIAEX II Gel Extraction Kit (150)                                                                       20021

QIAprep Spin Miniprep Kit (250)                                                                         27106

QIAGEN Plasmid Midi Kit (25)                                                                             12143

Type-it CNV SYBR Green PCR +qC Kit (100)                                                       206672

mericon Horse Kit (24)                                                                                   292143

MinElute PCR Purification Kit (250)                                                                    28006

Investigator Quantiplex Kit (200)                                                                     387016

RNeasy Mini Kit (50)                                                                                       74104

QIAGEN Plasmid Giga Kit (5)                                                                             12191

QIAfilter Plasmid Mega Kit (5)                                                                            12281

QIAfilter Plasmid Mega Kit (5)                                                                            12281

QuantiTect SYBR Green PCR Kit (200)                                                               204143

QIAamp DNA Mini Kit (50)                                                                                51304

Taq PCR Core Kit (1000)                                                                                   201225

DNeasy Blood & Tissue Kit (50)                                                                          69504

EpiTect® Methyl II DNA Restriction Kit                                                               335452

Omniscript RT Kit (50)                                                                                     205111

Taq PCR Master Mix Kit (1000 Units)                                                                  201445

RNeasy Protect Mini Kit (50)                                                                             74124

DyeEx 96 Kit (4)                                                                                            63181

QIAEX II Gel Extraction Kit (500)                                                                     20051

QIAGEN Large-Construct Kit (10)                                                                       12462

QIAfilter Plasmid Giga Kit (5)                                                                           12291

miScript Microfluidics PCR Kit (5)                                                                     331431

Type-it CNV Probe PCR +qC Kit (100)                                                               206662

RGS.His HRP Conjugate Kit                                                                            34450

MinElute Gel Extraction Kit (250)                                                                     28606

QuantiTect SYBR Green PCR Kit (40)                                                              204141

MinElute Reaction Cleanup Kit (250)                                                                28206

QIAGEN OneStep RT-PCR Kit (25)                                                                210210

RNeasy Midi Kit (10)                                                                                    75142

REPLI-g Mitochondrial DNA Kit (25)                                                                151023

PyroMark OneStep RT-PCR Kit (50)                                                               978801

HiSpeed Plasmid Maxi Kit (10)                                                                       12662

QIAprep Spin Miniprep Kit (50)                                                                      27104

RNeasy Plant Mini Kit (50)                                                                            74904

Oligotex mRNA Mini Kit                                                                                 70022

QIAamp DNA Blood Maxi Kit (10)                                                                   51192

QIAamp DNA Blood Midi Kit (20)                                                                    51183

virotype Influenza A RT-PCR Kit (24)                                                               282603

QIAamp DNA Blood Mini Kit (250)                                                                  51106

virotype BVDV RT-PCR Kit (24)                                                                      280373

QuantiTect Probe RT-PCR Kit (200)                                                                204443

QIAGEN PCR Cloning plus Kit (10)                                                                  231222

HotStarTaq Master Mix Kit (250)                                                                   203443

RNeasy Maxi Kit (12)                                                                                    75162

QIAquick Nucleotide Removal Kit (50)                                                            28304

Blood & Cell Culture DNA Maxi Kit                                                                  13362

Type-it CNV SYBR Green PCR Core Kit(400)                                                     206624

DNeasy Plant Maxi Kit (24)                                                                          68163

HiSpeed Plasmid Midi Kit (25)                                                                      12643

miRNeasy Serum/Plasma Kit (50)                                                                  217184

miRNeasy Micro Kit (50)                                                                              217084

QIAamp DNA Microbiome Kit (50)                                                                51704

NeXtal CubicPhase Kit                                                                               130807

DirectPrep 96 BioRobot Kit (4)                                                                   962341

Taq PCR Master Mix Kit (250)                                                                     201443

Binding Reagent (VXB) (350 ml)                                                                  950233

Rotor-Gene Multiplex RT-PCR Kit (80)                                                            204972

EZ1 DNA Investigator Kit (48)                                                                    952034

AllPrep RNA Protein Kit (50)                                                                          80404

QIAamp UltraSens Virus Kit (50)                                                                   53704

QIAGEN Fast Cycling PCR Kit (50)                                                                  203741

Gentra Puregene Cell Kit (2 x 108)                                                               158745

HotStarTaq Plus Master Mix Kit (250)                                                             203643

QuantiTect Probe PCR Kit (200)                                                                    204343

FlexiGene DNA Kit (250)                                                                             51206

Type-it HRM PCR Kit (100)                                                                         206542

QIAGEN LongRange PCR Kit (20)                                                                206401

REPLI-g Mini Kit (25)                                                                                  150023

EpiTect HRM PCR Kit (100)                                                                         59445

REPLI-g Midi Kit (25)                                                                                150043

miScript PreAMP PCR Kit (12)                                                                     331451

virotype SBV RT-PCR Kit (24)                                                                     281603

TAGZyme Kit                                                                                           34300

virotype BTV pan/8 RT-PCR Kit (24)                                                           280443

virotype PRRSV RT-PCR Kit (24)                                                                 282303

QIAGEN Plasmid Plus Mega Kit (5)                                                              12981

QIAGEN LongRange 2Step RT-PCR Kit (100)                                               205922

HotStar HiFidelity Polymerase Kit (100)                                                         202602

RNeasy Micro Kit (50)                                                                               74004

RT² PreAMP cDNA Synthesis Kit (12)                                                          330451

QIAGEN LongRange 2Step RT-PCR Kit (20)                                                  205920

Gentra Puregene Buccal Cell Kit (100)                                                         158845

Gentra Puregene Tissue Kit (4 g)                                                               158667

GeneRead DNAseq Panel PCR Kit V2 (12)                                                      181940

RT² miRNA First Strand Kit (12)                                                                   331401

QIAGEN Plasmid Plus Midi Sample Kit (5                                                          12941

QIAGEN Fast Cycling PCR Kit (200)                                                                203743

RNeasy Plus Mini Kit (50)                                                                              74134

QIAGEN Plasmid Plus Midi Kit (25)                                                                 12943

Gentra Puregene Cell Kit (8 x 108)                                                               158767

EZ1 RNA Tissue Mini Kit (48)                                                                        959034

virotype CSFV RT-PCR Kit (24)                                                                       281803

MagAttract DNA Mini M48 Kit (192)                                                                  953336

QuantiFast Multiplex RT-PCR +R Kit (80)                                                            204952

QIAGEN Plasmid Plus Giga Kit (5)                                                                        12991

QIAGEN Plasmid Plus Giga Kit (5)                                                                      12991

Gentra Puregene Blood Kit (30 ml)                                                                    158445

AllPrep DNA/RNA Mini Kit (50)                                                                               80204

RNeasy MinElute Cleanup Kit (50)                                                                     74204

QuantiFast Multiplex RT-PCR Kit (80)                                                                    204852

Gentra Puregene Mouse Tail Kit (4 g)                                                                  158267

RT² Easy First Strand Kit (12)                                                                             330421

Qproteome FFPE Tissue Kit (20)                                                                          37623

EZ1 RNA Universal Tissue Kit (48)                                                                          956034

QIAamp DNA Micro Kit (50)                                                                                  56304

RNeasy Fibrous Tissue Mini Kit (50)                                                                        74704

RT² SYBR Green qPCR Mastermix (2)                                                                    330500

Carrier, tubes (1), sample track, Qsym                                                                   9018700

QIAGEN LongRange PCR Kit (100)                                                                           206402

RBC Lysis Solution(450 ml)                                                                                    158902

RT² First Strand Kit (12)                                                                                     330401

RNase A Solution (650 µl)                                                                                     158922

Protein Precipitation Solution (350 ml)                                                                     158912

Puregene Blood Core Kit C                                                                                 158422

Large 96-Rod Cover (16)                                                                                       1031668

96-Well Microplate MP (20)                                                                                  1031656

EZ1 DSP Virus Kit (48)                                                                                         62724

EZ1 DNA Tissue Kit (48)                                                                                      953034

QIAzol Lysis Reagent (200 ml)                                                                              79306

RNeasy Lipid Tissue Mini Kit (50)                                                                            74804

RT² qPCR Primer gDNA Control (200)                                                                     330011

EpiTect ChIP Antibody Kit (12)                                                                              334481

artus Infl./H1 LC/RG RT-PCR Kit (24) RUO                                                                4523003

EZ1 Adv.XL DNA Investigator Card, europe                                                               9018699

EZ1 Adv. XL DNA Tissue Card                                                                                 9018701

EZ1 Adv. XL DNA Blood Card                                                                                   9018695

Gentra Puregene Yeast/Bact. Kit (200 ml)                                                                158567

Custom Cignal Finder Reporter Array                                                                        336835

EpiTect ChIP OneDay Kit (12)                                                                               334471

 Custom Mix-n-Match Multi-Analyte ELISArray Kits                                                     336111

HotStarTaq Plus DNA Polymerase (1000)                                                               203605

HotStarTaq Plus Master Mix Kit (2500)                                                                   203646
PAXgene Blood RNA Kit (50), CE                                                                       762174

EZ1 RNA Cell Mini Kit (48)                                                                                958034

全血RNA纯化试剂盒                                                                                         52304

产品详细信息

EZ1 DNA Blood 350 µl Kit (48)               951054

miRNeasy FFPE Kit (50)                     217504

Qproteome Albumin/IgG Depletion Kit    37521

QIAGEN Multiplex PCR Plus Kit (30)             206151

QIAquick Nucleotide Removal Kit (250)          28306

QIAGEN OneStep RT-PCR Kit (100)              210212

HotStarTaq Master Mix Kit (1000)                  203445

miScript SYBR Green PCR Kit (200)                 218073

GeneRead DNA Library I Core Kit (12)               180432

mericon Ruminant Kit (24)                                292073

mericon Vibrio triple Kit (24)                             290133

PyroMark Q24 Advanced Kit (4×24)                  970902

PAXgene TissueFIX Cont. (50 ml) Kit              765312

Shucker Finger Kit                                       9237290

QIAGEN Multiplex PCR Kit (100)                      206143

Allprep FFPE DNA/ RNA Kit (50)                    80234

Buffer Set (RNeasy 96 BioRobot 9604 Kit)            1019992

GeneRead DNA Library I Amp Kit (100)                180455

GeneRead DNA Library L Amp Kit (100)                 180485

EpiTect Plus LyseAll Bisulfite Kit (48)                     59164

mericon Y. enterocolitica Kit (24)                           290113

mericon Salmonella spp Kit (24)                             290013

QuantiFast Probe RT-PCR PLUS Kit (80)                     204482

RNeasy Protect Cell Mini Kit (50)                          74624

EpiTect Plus DNA Bisulfite Kit (48)                              59124

Qproteome Plasma Membrane Protein Kit                    37601

Rotor-Gene Type-it HRM Discovery Kit                          206541

EZ1 DNA Blood 200 µl Kit (48)                                    951034

GeneRead Size Selection Kit (50)                                  180514

EpiTect Plus FFPE Bisulfite Kit (48)                                  59144

QuantiFast Probe RT-PCR PLUS Kit (400)                           204484

QuantiTect Virus +ROX Vial Kit (50)                                  211031

mericon Sheep Kit (24)                                                   292063

mericon L. monocytogenes Kit (24)                                    290023

miRNeasy Mini Kit (50)                                                       217004

QIAamp DSP Virus Spin Kit (50)                                          61704

mericon Screen nos Kit (24)                                             291043

mericon Cronobacter spp Kit (24)                                        290063

PAXgene Blood DNA Kit (25)                                             761133

RNeasy Plus Micro Kit (50)                                                  74034

QuantiTect Virus Kit (50)                                                     211011

MinElute 96 UF PCR Purification Kit (4)                                   28051

QuantiTect Multiplex PCR NoROX Kit (200)                           204743

PAXgene Blood DNA Kit (25)                                             761133

RNeasy Plus Micro Kit (50)                                                  74034

QuantiTect Virus Kit (50)                                                    211011

MinElute 96 UF PCR Purification Kit (4)                                      28051

mericon Campylobacter triple Kit (24)                                         290043

mericon Screen 35S Kit (24)                                                     291013

QuantiFast Probe RT-PCR+ROXvial Kit(400)                                  204554

GeneRead rRNA Depletion Kit (6)                                              180211

mericon Goat Kit (24)                                                               292053

Type-it Fast SNP Probe PCR Kit (100)                                            206042

MagAttract DNA Blood Mini M48 Kit (192)                                       951336

GeneRead Library Quant Kit                                                           180612

GeneRead DNA Library L Core Kit (12)                                            180462

DyeEx 2.0 Spin Kit (50)                                                                 63204

mericon DNA Bacteria Plus Kit (50)                                                    69534

Type-it HRM PCR Kit (400)                                                               206544

QuantiFast Pathogen PCR +IC Kit (100)                                             211352

QuantiFast Pathogen RT-PCR +IC Kit (100)                                         211452

DNeasy mericon Food Kit (50)                                                          69514

EZ1 DSP DNA BLD Card                                                                     37571

CompactPrep Plasmid Maxi Kit (25)                                                       12863

Effectene Transfection Reag. Kit (0.3ml)                                               1054250

Qproteome Nuclear Protein Kit                                                               37582

cador T. equigenitalis PCR Kit                                                                  285023

CompactPrep Plasmid Midi Kit (25)                                                            12843

mericon Soy Kit (24)                                                                              293013

mericon Shigella spp Kit (24)                                                                   290103

MagAttract Viral RNA M48 Kit (96)                                                          955235

MagAttract Viral RNA M48 Kit (96)                                                         955235

TopTaq Master Mix Kit (250)                                                                  200403

QuantiFast Multiplex PCR Kit (80)                                                             204652

Qproteome Mammalian Protein Prep Kit                                                    37901

BioSprint 96 DNA Plant Kit (576)                                                              941557

QIAamp cador Pathogen Mini Kit (50)                                                          54104

Type-it Microsatellite PCR Kit (200)                                                           206243

Type-it Mutation Detect PCR Kit (200)                                                       206343

Type-it Mutation Detect PCR Kit (70)                                                         206341

MagAttract HMW DNA Kit (48)                                                                 67563

Qproteome Murine Albumin Depletion Kit                                                     37591

Microbial DNA qPCR Assay Kits                                                                    330033

BioSprint 15 DNA Blood Kit (45)                                                                940014

miScript Plant RT Kit (50)                                                                          218761

QIAamp UCP Pathogen Mini Kit (50),G                                                           50214

PAXgene Tissue RNA Kit (50)                                                                    765134

QIAGEN Multiplex PCR Plus Kit (100)                                                             206152

QuantiTect Multiplex PCR Kit (200)                                                               204543

EpiTect MSP PCR Kit (100)                                                                             59305

QuantiTect Rev.Transcription Kit (50)                                                                205311

QuantiTect Rev.Transcription Kit (10)                                                                205310

QIAamp DNA FFPE Tissue Kit (50)                                                                    56404

PAXgene Tissue DNA Kit (50)                                                                          767134

QIAamp Fast DNA Stool Mini Kit (50)                                                                   51604

QuantiNova SYBR Green PCR Kit (500)                                                           208054

QuantiTect Probe PCR + UNG Kit (200)                                                          204363

Rotor-Gene Probe RT-PCR Kit (400)                                                               204574

QuantiTect Multiplex PCR NoROX Kit (40)                                                        204741

EpiTect Fast DNA Bisulfite Kit (50)                                                                  59824

Ni-NTA Spin Kit (50)                                                                                      31314

QIAprep Spin M13 Kit (50)                                                                             27704

Oligotex Direct mRNA Mini Kit                                                                          72022

QuantiNova SYBR Green PCR Kit (100)                                                             208052

QuantiTect Probe PCR Kit (40)                                                                       204341

NeXtal Stock Kit Cryo                                                                                      132981

QIAamp UCP PurePathogen Blood Kit (10)                                                          50112

QuickLyse Miniprep Kit (100)                                                                            27405

GeneRead DNA FFPE Kit (50)                                                                           180134

QIAvac Holder Kit                                                                                          19418

QuantiTect Multiplex PCR Kit (40)                                                                    204541

EpiTect Fast LyseAll Bisulfite Kit (50)                                                                   59864

miScript II RT Kit (12)                                                                                     218160

miScript PCR Starter Kit (80)                                                                                218193

Penta.His HRP Conjugate Kit                                                                                  34460

QIAquick PCR Purification Kit (50)                                                                            28104

QIAfilter Plasmid Maxi Kit (10)                                                                                   12262

Anti·His Antibody Selector Kit                                                                                  34698

QIAquick Gel Extraction Kit (50)                                                                            28704

MinElute PCR Purification Kit (50)                                                                          28004

QIAfilter Plasmid Midi Kit (25)                                                                              12243

QIAGEN Plasmid Mini Kit (25)                                                                              12123

QIAGEN PCR Cloning Kit (40)                                                                              231124

RNeasy Plant Mini Kit (20)                                                                                   74903

Ni-NTA Superflow Cartridges (5×1 ml)                                                                  30721

QIAquick Gel Extraction Kit (250)                                                                         28706

Blood & Cell Culture DNA Mini Kit                                                                           13323

QIAGEN Plasmid Mini Kit (100)                                                                              12125

EndoFree Plasmid Giga Kit (5)                                                                               12391

NeXtal Stock Kit Salt                                                                                            132985

QuickLyse Miniprep Kit (250)                                                                                  27406

QuantiTect SYBR Green PCR +UNG Kit (200)                                                         204163

QIAGEN Plasmid Maxi Kit (10)                                                                              12162

EndoFree Plasmid Maxi Kit (10)                                                                            12362

EpiTect Fast FFPE Bisulfite Kit (50)                                                                       59844

REPLI-g Human Control Kit                                                                                 150090

RNeasy Protect Animal Blood Kit (50)                                                                  1054578

QIAamp MinElute Media Kit (50)                                                                         57414

MinElute Gel Extraction Kit (50)                                                                          28604

Blood & Cell Culture DNA Midi Kit                                                                         13343

EndoFree Plasmid Mega Kit (5)                                                                           12381

PAXgene Bone Marrow RNA Kit (30)                                                                   764133

miScript II RT Kit (50)                                                                                       218161

Omniscript RT Kit (10)                                                                                     205110

QIAamp DNA Stool Mini Kit (50)                                                                           51504

QIAquick PCR Purification Kit (250)                                                                     28106

QIAEX II Gel Extraction Kit (150)                                                                       20021

QIAprep Spin Miniprep Kit (250)                                                                         27106

QIAGEN Plasmid Midi Kit (25)                                                                             12143

Type-it CNV SYBR Green PCR +qC Kit (100)                                                       206672

mericon Horse Kit (24)                                                                                   292143

MinElute PCR Purification Kit (250)                                                                    28006

Investigator Quantiplex Kit (200)                                                                     387016

RNeasy Mini Kit (50)                                                                                       74104

QIAGEN Plasmid Giga Kit (5)                                                                             12191

QIAfilter Plasmid Mega Kit (5)                                                                            12281

QIAfilter Plasmid Mega Kit (5)                                                                            12281

QuantiTect SYBR Green PCR Kit (200)                                                               204143

QIAamp DNA Mini Kit (50)                                                                                51304

Taq PCR Core Kit (1000)                                                                                   201225

DNeasy Blood & Tissue Kit (50)                                                                          69504

EpiTect® Methyl II DNA Restriction Kit                                                               335452

Omniscript RT Kit (50)                                                                                     205111

Taq PCR Master Mix Kit (1000 Units)                                                                  201445

RNeasy Protect Mini Kit (50)                                                                             74124

DyeEx 96 Kit (4)                                                                                            63181

QIAEX II Gel Extraction Kit (500)                                                                     20051

QIAGEN Large-Construct Kit (10)                                                                       12462

QIAfilter Plasmid Giga Kit (5)                                                                           12291

miScript Microfluidics PCR Kit (5)                                                                     331431

Type-it CNV Probe PCR +qC Kit (100)                                                               206662

RGS.His HRP Conjugate Kit                                                                            34450

MinElute Gel Extraction Kit (250)                                                                     28606

QuantiTect SYBR Green PCR Kit (40)                                                              204141

MinElute Reaction Cleanup Kit (250)                                                                28206

QIAGEN OneStep RT-PCR Kit (25)                                                                210210

RNeasy Midi Kit (10)                                                                                    75142

REPLI-g Mitochondrial DNA Kit (25)                                                                151023

PyroMark OneStep RT-PCR Kit (50)                                                               978801

HiSpeed Plasmid Maxi Kit (10)                                                                       12662

QIAprep Spin Miniprep Kit (50)                                                                      27104

RNeasy Plant Mini Kit (50)                                                                            74904

Oligotex mRNA Mini Kit                                                                                 70022

QIAamp DNA Blood Maxi Kit (10)                                                                   51192

QIAamp DNA Blood Midi Kit (20)                                                                    51183

virotype Influenza A RT-PCR Kit (24)                                                               282603

QIAamp DNA Blood Mini Kit (250)                                                                  51106

virotype BVDV RT-PCR Kit (24)                                                                      280373

QuantiTect Probe RT-PCR Kit (200)                                                                204443

QIAGEN PCR Cloning plus Kit (10)                                                                  231222

HotStarTaq Master Mix Kit (250)                                                                   203443

RNeasy Maxi Kit (12)                                                                                    75162

QIAquick Nucleotide Removal Kit (50)                                                            28304

Blood & Cell Culture DNA Maxi Kit                                                                  13362

Type-it CNV SYBR Green PCR Core Kit(400)                                                     206624

DNeasy Plant Maxi Kit (24)                                                                          68163

HiSpeed Plasmid Midi Kit (25)                                                                      12643

miRNeasy Serum/Plasma Kit (50)                                                                  217184

miRNeasy Micro Kit (50)                                                                              217084

QIAamp DNA Microbiome Kit (50)                                                                51704

NeXtal CubicPhase Kit                                                                               130807

DirectPrep 96 BioRobot Kit (4)                                                                   962341

Taq PCR Master Mix Kit (250)                                                                     201443

Binding Reagent (VXB) (350 ml)                                                                  950233

Rotor-Gene Multiplex RT-PCR Kit (80)                                                            204972

EZ1 DNA Investigator Kit (48)                                                                    952034

AllPrep RNA Protein Kit (50)                                                                          80404

QIAamp UltraSens Virus Kit (50)                                                                   53704

QIAGEN Fast Cycling PCR Kit (50)                                                                  203741

Gentra Puregene Cell Kit (2 x 108)                                                               158745

HotStarTaq Plus Master Mix Kit (250)                                                             203643

QuantiTect Probe PCR Kit (200)                                                                    204343

FlexiGene DNA Kit (250)                                                                             51206

Type-it HRM PCR Kit (100)                                                                         206542

QIAGEN LongRange PCR Kit (20)                                                                206401

REPLI-g Mini Kit (25)                                                                                  150023

EpiTect HRM PCR Kit (100)                                                                         59445

REPLI-g Midi Kit (25)                                                                                150043

miScript PreAMP PCR Kit (12)                                                                     331451

virotype SBV RT-PCR Kit (24)                                                                     281603

TAGZyme Kit                                                                                           34300

virotype BTV pan/8 RT-PCR Kit (24)                                                           280443

virotype PRRSV RT-PCR Kit (24)                                                                 282303

QIAGEN Plasmid Plus Mega Kit (5)                                                              12981

QIAGEN LongRange 2Step RT-PCR Kit (100)                                               205922

HotStar HiFidelity Polymerase Kit (100)                                                         202602

RNeasy Micro Kit (50)                                                                               74004

RT² PreAMP cDNA Synthesis Kit (12)                                                          330451

QIAGEN LongRange 2Step RT-PCR Kit (20)                                                  205920

Gentra Puregene Buccal Cell Kit (100)                                                         158845

Gentra Puregene Tissue Kit (4 g)                                                               158667

GeneRead DNAseq Panel PCR Kit V2 (12)                                                      181940

RT² miRNA First Strand Kit (12)                                                                   331401

QIAGEN Plasmid Plus Midi Sample Kit (5                                                          12941

QIAGEN Fast Cycling PCR Kit (200)                                                                203743

RNeasy Plus Mini Kit (50)                                                                              74134

QIAGEN Plasmid Plus Midi Kit (25)                                                                 12943

Gentra Puregene Cell Kit (8 x 108)                                                               158767

EZ1 RNA Tissue Mini Kit (48)                                                                        959034

virotype CSFV RT-PCR Kit (24)                                                                       281803

MagAttract DNA Mini M48 Kit (192)                                                                  953336

QuantiFast Multiplex RT-PCR +R Kit (80)                                                            204952

QIAGEN Plasmid Plus Giga Kit (5)                                                                        12991

QIAGEN Plasmid Plus Giga Kit (5)                                                                      12991

Gentra Puregene Blood Kit (30 ml)                                                                    158445

AllPrep DNA/RNA Mini Kit (50)                                                                               80204

RNeasy MinElute Cleanup Kit (50)                                                                     74204

QuantiFast Multiplex RT-PCR Kit (80)                                                                    204852

Gentra Puregene Mouse Tail Kit (4 g)                                                                  158267

RT² Easy First Strand Kit (12)                                                                             330421

Qproteome FFPE Tissue Kit (20)                                                                          37623

EZ1 RNA Universal Tissue Kit (48)                                                                          956034

QIAamp DNA Micro Kit (50)                                                                                  56304

RNeasy Fibrous Tissue Mini Kit (50)                                                                        74704

RT² SYBR Green qPCR Mastermix (2)                                                                    330500

Carrier, tubes (1), sample track, Qsym                                                                   9018700

QIAGEN LongRange PCR Kit (100)                                                                           206402

RBC Lysis Solution(450 ml)                                                                                    158902

RT² First Strand Kit (12)                                                                                     330401

RNase A Solution (650 µl)                                                                                     158922

Protein Precipitation Solution (350 ml)                                                                     158912

Puregene Blood Core Kit C                                                                                 158422

Large 96-Rod Cover (16)                                                                                       1031668

96-Well Microplate MP (20)                                                                                  1031656

EZ1 DSP Virus Kit (48)                                                                                         62724

EZ1 DNA Tissue Kit (48)                                                                                      953034

QIAzol Lysis Reagent (200 ml)                                                                              79306

RNeasy Lipid Tissue Mini Kit (50)                                                                            74804

RT² qPCR Primer gDNA Control (200)                                                                     330011

EpiTect ChIP Antibody Kit (12)                                                                              334481

artus Infl./H1 LC/RG RT-PCR Kit (24) RUO                                                                4523003

EZ1 Adv.XL DNA Investigator Card, europe                                                               9018699

EZ1 Adv. XL DNA Tissue Card                                                                                 9018701

EZ1 Adv. XL DNA Blood Card                                                                                   9018695

Gentra Puregene Yeast/Bact. Kit (200 ml)                                                                158567

Custom Cignal Finder Reporter Array                                                                        336835

EpiTect ChIP OneDay Kit (12)                                                                               334471

 Custom Mix-n-Match Multi-Analyte ELISArray Kits                                                     336111

HotStarTaq Plus DNA Polymerase (1000)                                                               203605

HotStarTaq Plus Master Mix Kit (2500)                                                                   203646
PAXgene Blood RNA Kit (50), CE                                                                       762174

EZ1 RNA Cell Mini Kit (48)                                                                                958034

全血RNA纯化试剂盒                                                                                         52304

QIAGEN Multiplex PCR Plus Kit (30) 货号:206151

QIAGEN Multiplex PCR Plus Kit (30) 货号:206151

QIAGEN Multiplex PCR Plus Kit (30)

货  号:206151

产品规格:30

原  产  地:德国

参考价格:1050 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Multiplex PCR Plus Kit (30)

206151

用于成功的进行多重PCR反应

  • 成功的进行多重PCR反应,适用于高要求应用
  • 稳定、可靠的结果
  • 快速建立多重PCR反应体系,无需优化
  • 反应时间缩短,更加便利
QIAGEN Multiplex PCR Plus Kit专为便利、灵敏的多重PCR设计,无需优化。该试剂盒基于专有的多重PCR技术,能够在首次尝试即获得成功的结果,且比以往更快更便利。该试剂盒提 供便利的预混液。预混液独特的成分确保了反应的高特异性和高灵敏度。该预混液包含新型的缓冲液,专为多重PCR研发。该试剂盒还提供新型的辅助剂Q- Solution,有助于扩增难以扩增的片段,如富含GC的区域或具复杂二级结构的模板。该试剂盒还提供CoralLoad Dye,确保DNA迁移的操作便利且可视。

原理

QIAGEN Multiplex PCR Plus Kit基于QIAGEN专有的多重PCR技术,含有即用型混合液。该试剂盒所有的成分专为提供最易操作和最大速度研发,提供一致可靠的结果(参见下表)。Multiplex PCR Master Mix包含优化的HotStarTaq Plus DNA Polymerase和MgCl2浓度,以及dNTP和新型的PCR缓冲液。

多重PCR成功的关键因素,用于各种应用的一致可靠的结果

Multiplex PCR为研究者节省时间和成本用于进行大量的PCR反应,广泛应用于基因分型以及在研究、法医和分子检测实验室的DNA或cDNA检测应用。应用范围包括 分型和转基因生物和病原体的分析、微卫星的扩增分析、检测SNP突变区域,以及宏基因组学研究。然而,多重PCR技术要求严格。PCR优化是多重PCR成 功与否的关键因素。多重PCR中不同引物对不同杂交动力学可能导致如下问题,如扩增偏向性。能高效率结合的引物,更多地利用扩增反应元件,从而降低了其他 PCR产物的产量。由于更大的引物量,因此存在更大的形成引物二聚体和非特异性启动的风险。不解决这些风险导致低灵敏度、非特异性扩增和选择靶位的偏向性 扩增,因此得到不一致的不令人满意的结果。共格这些瓶颈需要繁琐耗时的优化步骤,导致成本的增加。QIAGEN Multiplex PCR Plus Kit消除了这些风险,在首次尝试时就运行顺利。Multiplex PCR Master Mix独特的组成确保了高特异性和灵敏度的扩增反应,甚至是难扩增的靶位。该试剂盒能快速成功地建立所有多重PCR检测,有效的节省时间和成本(参见Successful multiplex PCR without the need for optimization)。

HotStarTaq Plus DNA Polymerase

HotStarTaq Plus DNA PolymeraseTaq DNA聚合酶的优化形式,在室温下无聚合酶活性。这能防止在PCR设置和PCR初始循环过程中,低温下非特异性退货引物的延伸和引物二聚体的形成。无需优 化反应条件。在室温下可方便设置多重检测。HotStarTaq DNA Polymerase在95°C下孵育5分钟激活,可整合入现有的各种热循环程序中。

多重PCR缓冲液

这特殊的缓冲液包含优化的混合物由K+和NH4+以及独特的PCR辅助剂、MP因子组成可增加模板引物的局部浓度(参见Stable and efficient annealing)。将非特异性退火最小化并成功地平行扩增所有靶位,甚至只有非常少量的模板。配合K+和其他阳离子、MP因子稳定特异性结合的引物,并能够用HotStarTaq Plus DNA Polymerase进行高效的引物延伸。通过在每个PCR循环中高比率的特异性-非特异性引物结合,该新型的缓冲液维持每个PCR循环的特异性扩增。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该缓冲液能在更广泛的退火温度和Mg2+浓度下,提供严格的引物退火条件。通过改变退火温度或Mg2+浓度可优化PCR,因此通常将优化过程最小化或无需要优化。

该试剂盒包含独特的辅助剂Q-Solution,促进难扩增的靶位扩增如富含GC的区域或具有复杂二级结构的模板。该试剂盒还含有高度灵活的CoralLoad Dye,通过提高移液器的可视性和后续的凝胶上样以及DNA迁移的可视性,来进一步提高操作的方便性(参见Easy PCR setup and convenient DNA visualization)。

QIAGEN Multiplex PCR Plus Kit的优点
试剂盒组分 优点
HotStarTaq Plus DNA Polymerase 高特异性和灵敏度扩增
室温设置
5分钟激活时间
Multiplex PCR Plus Buffer with Factor MP 平行扩增所有靶位
无需优化PCR参数
单一的用于所有多重检测的实验方案
快速的操作流程
CoralLoad Dye 移液过程中简单的可视化
可即可凝胶上样
DNA迁移的可视化
Q-Solution 扩增困难靶位

操作流程

QIAGEN Multiplex PCR Plus Kit能够在首次尝试就可成功进行多重PCR。

简单快速的下游分析

QIAGEN Multiplex PCR Plus Kit使用多种方法得到的多重PCR产物的下游分析简单快速。无论分析是否使用琼脂糖凝胶电泳,毛细管电泳或是否使用QIAxcel Advanced System,所有阳离子可方便的可视化,单独的片段也可有效区别。

便利的试剂盒规格

QIAGEN Multiplex PCR Plus Kit提供更方便的即用型预优化混合液。使用混合物节省时间、简化反应设置操作,并通过消除众多移液失误和污染的可能性来增加可靠性,将移液步骤最小化并 去除繁琐的计算。只需将引物和模板加入来制备最终扩增混合物。混合液可在2–8°C下储存,可更快速的设置多重PCR检测。可在室温下设置反应,操作简 便,易于使用。HotStarTaq Plus DNA Polymerase在95°C经5分钟孵育激活,可整合入现有的热循环程序中。试剂盒中包含的CoralLoad Dye便于使用,提高PCR设置中移液的可视化和凝胶上DNA迁移的可视化。

应用
QIAGEN Multiplex PCR Plus Kit能成功应用于多种应用,从转基因生物分型、病原体检测到癌症研究、宏基因组学和基因表达分析。

产品详细信息

QIAGEN Multiplex PCR Plus Kit (30)

206151

用于成功的进行多重PCR反应

  • 成功的进行多重PCR反应,适用于高要求应用
  • 稳定、可靠的结果
  • 快速建立多重PCR反应体系,无需优化
  • 反应时间缩短,更加便利
QIAGEN Multiplex PCR Plus Kit专为便利、灵敏的多重PCR设计,无需优化。该试剂盒基于专有的多重PCR技术,能够在首次尝试即获得成功的结果,且比以往更快更便利。该试剂盒提 供便利的预混液。预混液独特的成分确保了反应的高特异性和高灵敏度。该预混液包含新型的缓冲液,专为多重PCR研发。该试剂盒还提供新型的辅助剂Q- Solution,有助于扩增难以扩增的片段,如富含GC的区域或具复杂二级结构的模板。该试剂盒还提供CoralLoad Dye,确保DNA迁移的操作便利且可视。

原理

QIAGEN Multiplex PCR Plus Kit基于QIAGEN专有的多重PCR技术,含有即用型混合液。该试剂盒所有的成分专为提供最易操作和最大速度研发,提供一致可靠的结果(参见下表)。Multiplex PCR Master Mix包含优化的HotStarTaq Plus DNA Polymerase和MgCl2浓度,以及dNTP和新型的PCR缓冲液。

多重PCR成功的关键因素,用于各种应用的一致可靠的结果

Multiplex PCR为研究者节省时间和成本用于进行大量的PCR反应,广泛应用于基因分型以及在研究、法医和分子检测实验室的DNA或cDNA检测应用。应用范围包括 分型和转基因生物和病原体的分析、微卫星的扩增分析、检测SNP突变区域,以及宏基因组学研究。然而,多重PCR技术要求严格。PCR优化是多重PCR成 功与否的关键因素。多重PCR中不同引物对不同杂交动力学可能导致如下问题,如扩增偏向性。能高效率结合的引物,更多地利用扩增反应元件,从而降低了其他 PCR产物的产量。由于更大的引物量,因此存在更大的形成引物二聚体和非特异性启动的风险。不解决这些风险导致低灵敏度、非特异性扩增和选择靶位的偏向性 扩增,因此得到不一致的不令人满意的结果。共格这些瓶颈需要繁琐耗时的优化步骤,导致成本的增加。QIAGEN Multiplex PCR Plus Kit消除了这些风险,在首次尝试时就运行顺利。Multiplex PCR Master Mix独特的组成确保了高特异性和灵敏度的扩增反应,甚至是难扩增的靶位。该试剂盒能快速成功地建立所有多重PCR检测,有效的节省时间和成本(参见Successful multiplex PCR without the need for optimization)。

HotStarTaq Plus DNA Polymerase

HotStarTaq Plus DNA PolymeraseTaq DNA聚合酶的优化形式,在室温下无聚合酶活性。这能防止在PCR设置和PCR初始循环过程中,低温下非特异性退货引物的延伸和引物二聚体的形成。无需优 化反应条件。在室温下可方便设置多重检测。HotStarTaq DNA Polymerase在95°C下孵育5分钟激活,可整合入现有的各种热循环程序中。

多重PCR缓冲液

这特殊的缓冲液包含优化的混合物由K+和NH4+以及独特的PCR辅助剂、MP因子组成可增加模板引物的局部浓度(参见Stable and efficient annealing)。将非特异性退火最小化并成功地平行扩增所有靶位,甚至只有非常少量的模板。配合K+和其他阳离子、MP因子稳定特异性结合的引物,并能够用HotStarTaq Plus DNA Polymerase进行高效的引物延伸。通过在每个PCR循环中高比率的特异性-非特异性引物结合,该新型的缓冲液维持每个PCR循环的特异性扩增。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该缓冲液能在更广泛的退火温度和Mg2+浓度下,提供严格的引物退火条件。通过改变退火温度或Mg2+浓度可优化PCR,因此通常将优化过程最小化或无需要优化。

该试剂盒包含独特的辅助剂Q-Solution,促进难扩增的靶位扩增如富含GC的区域或具有复杂二级结构的模板。该试剂盒还含有高度灵活的CoralLoad Dye,通过提高移液器的可视性和后续的凝胶上样以及DNA迁移的可视性,来进一步提高操作的方便性(参见Easy PCR setup and convenient DNA visualization)。

QIAGEN Multiplex PCR Plus Kit的优点
试剂盒组分 优点
HotStarTaq Plus DNA Polymerase 高特异性和灵敏度扩增
室温设置
5分钟激活时间
Multiplex PCR Plus Buffer with Factor MP 平行扩增所有靶位
无需优化PCR参数
单一的用于所有多重检测的实验方案
快速的操作流程
CoralLoad Dye 移液过程中简单的可视化
可即可凝胶上样
DNA迁移的可视化
Q-Solution 扩增困难靶位

操作流程

QIAGEN Multiplex PCR Plus Kit能够在首次尝试就可成功进行多重PCR。

简单快速的下游分析

QIAGEN Multiplex PCR Plus Kit使用多种方法得到的多重PCR产物的下游分析简单快速。无论分析是否使用琼脂糖凝胶电泳,毛细管电泳或是否使用QIAxcel Advanced System,所有阳离子可方便的可视化,单独的片段也可有效区别。

便利的试剂盒规格

QIAGEN Multiplex PCR Plus Kit提供更方便的即用型预优化混合液。使用混合物节省时间、简化反应设置操作,并通过消除众多移液失误和污染的可能性来增加可靠性,将移液步骤最小化并 去除繁琐的计算。只需将引物和模板加入来制备最终扩增混合物。混合液可在2–8°C下储存,可更快速的设置多重PCR检测。可在室温下设置反应,操作简 便,易于使用。HotStarTaq Plus DNA Polymerase在95°C经5分钟孵育激活,可整合入现有的热循环程序中。试剂盒中包含的CoralLoad Dye便于使用,提高PCR设置中移液的可视化和凝胶上DNA迁移的可视化。

应用
QIAGEN Multiplex PCR Plus Kit能成功应用于多种应用,从转基因生物分型、病原体检测到癌症研究、宏基因组学和基因表达分析。

QIAGEN OneStep RT-PCR Kit (100) 货号:210212

QIAGEN OneStep RT-PCR Kit (100) 货号:210212

QIAGEN OneStep RT-PCR Kit (100)

货  号:210212

产品规格:100

原  产  地:德国

参考价格:6180 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN OneStep RT-PCR Kit (100)

210212

用于灵敏、特异性的一步法RT-PCR

  • 快速方便的单管反应
  • 适合各种RNA模板,无需优化反应条件
  • 独特的酶组合,确保反应的高特异性和高灵敏度
  • 经优化的缓冲体系确保了高效的逆转录和扩增过程
QIAGEN One-Step RT-PCR Kit包含Omniscript逆转录酶和Sensiscript逆转录酶的混合物、HotStarTaq DNA Polymerase、QIAGEN OneStep RT-PCR Buffer、dNTP混合液和Q-Solution。Q-Solution是一种新型的辅助剂,有助于实现“困难”模板(如:高GC含量的模板)的高效 扩增。简单的单管反应体系构建和优化的试剂盒组分确保了结果成功且灵敏度高。

原理

QIAGEN OneStep RT-PCR Kit可对多种RNA模板进行简单、灵敏的一步式RT-PCR。

QIAGEN OneStep RT-PCR Enzyme Mix含有为逆转录和PCR反应特别设计的酶。独特组合的Omniscript和Sensiscrip逆转录酶对RNA模板有高度亲和性,确保RNA逆转 录的灵敏度达到1 pg到2 µg。逆转录完成后,升温至95°C,15分钟,活化HotStarTaq DNA Polymerase同时使逆转录酶失活。这种方法可减少引物二聚体等非特异性扩增产物的生成,并减少背景的影响,确保RT-PCR的高灵敏度和可重复 性。

退火温度范围宽

引物的最佳退火温度取决于碱基对的组成(如A、T、G、C核苷酸的比例)、引物的浓度和反应的离子环境。QIAGEN PCR Buffers含有K+和NH4+,使用该缓冲液可在很宽的温度范围内增加特异性PCR产物的产量(参见Increased specific primer annealing)。其原理是破坏非特异性结合的引物对,提供更加可靠的反应环境,减少繁琐的温度优化过程。相反,如使用仅含K+的PCR或一步式RT-PCR缓冲液,PCR最佳退火温度的范围较窄,可靠性较差。

QIAGEN OneStep RT-PCR Buffer专为提高逆转录和PCR扩增的效率而设计。该缓冲液含有新型辅助剂,可防止逆转录酶对PCR扩增的抑制,而这是一步式RT-PCR中常见的问题。该缓冲液确保在较大的温度范围和Mg2+浓 度范围内,引物与模板特异性结合,可对多种RNA模板进行高效的RT-PCR扩增。QIAGEN OneStep RT-PCR Kit含有Q-Solution,这是可修饰核酸熔解行为的新型辅助剂。Q-Solution可促进高GC含量或含较多二级结构的模板的逆转录和扩增。 使用Q-Solution可简化复杂模板RT-PCR的优化过程。QIAGEN OneStep RT-PCR Kit含有您所需的所有试剂,可进行快速、简便的RT-PCR,用于多种灵敏的应用(见表)。

可靠的一步式RT-PCR结果
QIAGEN OneStep RT-PCR Kit 组分 特点
HotStarTaq DNA Polymerase 高度特异性产物
Sensiscript and Omniscript Reverse Transcriptases RNA初始量的范围大 (1 pg–2 µg)
高灵敏度
OneStep RT-PCR Buffer 无需优化
反转录酶不会抑制PCR
抑制RNases
Q-Solution 促进GC含量高的模板的扩增

 

操作流程

使用QIAGEN OneStep RT-PCR Kit可快速、简单的进行RT-PCR反应体系构建。无论是何种应用,即无论是病毒检测、分子诊断研究还是基因表达,您只需将所有试剂在一个离心管中混合即可开始PCR扩增(参见OneStep RT-PCR procedure)。反应混合液含有逆转录和PCR反应所需的所有试剂,无需添加其他试剂即可开始反应(参见下表)。

应用

QIAGEN OneStep RT-PCR Kit适用于RT-PCR分析,包括:

  • 病毒检测
  • 单细胞RT-PCR
  • 基因表达分析

产品详细信息

QIAGEN OneStep RT-PCR Kit (100)

210212

用于灵敏、特异性的一步法RT-PCR

  • 快速方便的单管反应
  • 适合各种RNA模板,无需优化反应条件
  • 独特的酶组合,确保反应的高特异性和高灵敏度
  • 经优化的缓冲体系确保了高效的逆转录和扩增过程
QIAGEN One-Step RT-PCR Kit包含Omniscript逆转录酶和Sensiscript逆转录酶的混合物、HotStarTaq DNA Polymerase、QIAGEN OneStep RT-PCR Buffer、dNTP混合液和Q-Solution。Q-Solution是一种新型的辅助剂,有助于实现“困难”模板(如:高GC含量的模板)的高效 扩增。简单的单管反应体系构建和优化的试剂盒组分确保了结果成功且灵敏度高。

原理

QIAGEN OneStep RT-PCR Kit可对多种RNA模板进行简单、灵敏的一步式RT-PCR。

QIAGEN OneStep RT-PCR Enzyme Mix含有为逆转录和PCR反应特别设计的酶。独特组合的Omniscript和Sensiscrip逆转录酶对RNA模板有高度亲和性,确保RNA逆转 录的灵敏度达到1 pg到2 µg。逆转录完成后,升温至95°C,15分钟,活化HotStarTaq DNA Polymerase同时使逆转录酶失活。这种方法可减少引物二聚体等非特异性扩增产物的生成,并减少背景的影响,确保RT-PCR的高灵敏度和可重复 性。

退火温度范围宽

引物的最佳退火温度取决于碱基对的组成(如A、T、G、C核苷酸的比例)、引物的浓度和反应的离子环境。QIAGEN PCR Buffers含有K+和NH4+,使用该缓冲液可在很宽的温度范围内增加特异性PCR产物的产量(参见Increased specific primer annealing)。其原理是破坏非特异性结合的引物对,提供更加可靠的反应环境,减少繁琐的温度优化过程。相反,如使用仅含K+的PCR或一步式RT-PCR缓冲液,PCR最佳退火温度的范围较窄,可靠性较差。

QIAGEN OneStep RT-PCR Buffer专为提高逆转录和PCR扩增的效率而设计。该缓冲液含有新型辅助剂,可防止逆转录酶对PCR扩增的抑制,而这是一步式RT-PCR中常见的问题。该缓冲液确保在较大的温度范围和Mg2+浓 度范围内,引物与模板特异性结合,可对多种RNA模板进行高效的RT-PCR扩增。QIAGEN OneStep RT-PCR Kit含有Q-Solution,这是可修饰核酸熔解行为的新型辅助剂。Q-Solution可促进高GC含量或含较多二级结构的模板的逆转录和扩增。 使用Q-Solution可简化复杂模板RT-PCR的优化过程。QIAGEN OneStep RT-PCR Kit含有您所需的所有试剂,可进行快速、简便的RT-PCR,用于多种灵敏的应用(见表)。

可靠的一步式RT-PCR结果
QIAGEN OneStep RT-PCR Kit 组分 特点
HotStarTaq DNA Polymerase 高度特异性产物
Sensiscript and Omniscript Reverse Transcriptases RNA初始量的范围大 (1 pg–2 µg)
高灵敏度
OneStep RT-PCR Buffer 无需优化
反转录酶不会抑制PCR
抑制RNases
Q-Solution 促进GC含量高的模板的扩增

 

操作流程

使用QIAGEN OneStep RT-PCR Kit可快速、简单的进行RT-PCR反应体系构建。无论是何种应用,即无论是病毒检测、分子诊断研究还是基因表达,您只需将所有试剂在一个离心管中混合即可开始PCR扩增(参见OneStep RT-PCR procedure)。反应混合液含有逆转录和PCR反应所需的所有试剂,无需添加其他试剂即可开始反应(参见下表)。

应用

QIAGEN OneStep RT-PCR Kit适用于RT-PCR分析,包括:

  • 病毒检测
  • 单细胞RT-PCR
  • 基因表达分析

QIAGEN Multiplex PCR Kit (100) 货号:206143

QIAGEN Multiplex PCR Kit (100) 货号:206143

QIAGEN Multiplex PCR Kit (100)

货  号:206143

产品规格:100

原  产  地:德国

参考价格:3490 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Multiplex PCR Kit (100)

206143

用于高特异性和灵敏度的多重PCR,无需优化

  • 无需优化步骤
  • 高特异性和高灵敏度,热启动
  • 高度适合用于各种类型的多重PCR应用
  • 使用简单,经济实惠

QIAGEN Multiplex PCR Kit提供方便的即用型预混液。QIAGEN Multiplex PCR Master Mix包含HotStarTaq DNA Polymerase和含有新型合成因子MP的独特PCR缓冲液。配合优化的盐浓度,MP因子可以促进引物与模板的特异性结合,使反应体系中所有的引物都 能有效延伸,无需额外优化。该试剂盒还包含新型的辅助剂Q-Solution,有助于实现“困难”模板(比如,GC含量高的模板)的高效扩增。

原理

QIAGEN Multiplex PCR Kit是首个专为多重PCR研发的试剂盒,有即用型混合液规格。QIAGEN Multiplex PCR Master Mix包含预优化浓度的HotStarTaq DNA Polymerase和MgCl2、dNTPs和新型的PCR缓冲液专为多重PCR研发。该试剂盒在首次尝试多重PCR即获得成功。由于试剂盒内独特的预优化的试剂无需优化反应条件(比如,引物浓度,Mg2+Taq DNA聚合酶)和循环参数。

HotStarTaq DNA Polymerase

HotStarTaq DNA Polymerase对 Taq DNA聚合酶进行改善,在室温下无聚合酶活性。这可以防止非特异性退火引物和在低温条件下的PCR设置和初始的PCR循环中形成引物二聚体。在95°C下,经15分钟诱导可激活HotStarTaq DNA Polymerase,可纳入各种现有的热循环程序中。

多重PCR缓冲液

这特殊的缓冲液包含优化的由K+和NH4+以及独特的PCR辅助剂、MP因子可增加模板引物的局部浓度。配合K+和其他阳离子、MP因子稳定特异性结合的引物,并能够用HotStarTaq DNA Polymerase进行高效的引物延伸(参见Stable and efficient primer annealing)。通过在每个PCR循环中高比率的特异性-非特异性引物结合,该新型的缓冲液维持每个PCR循环的特异性扩增。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该缓冲液能在更广泛的退火温度和Mg2+浓度下,提供严格的引物退火条件。通过改变退火温度或Mg2+浓度可优化PCR,因此通常将优化过程最小化或无需要优化。

Q-Solution

Q-Solution是一种新型的PCR辅助剂,有助于扩增由DNA融化特性修改的困难模板,同时配合HotStarTaq DNA Polymerase提供。该独特的试剂可优化高度二级结构或富含GC的模板所致的效果欠佳的PCR。不同于其他常用的PCR辅助剂如DMSO,Q-Solution只用于一种工作浓度,并保证无毒和PCR纯度好。

操作流程

QIAGEN Multiplex PCR Kit含有即用型、预优化的混合液,非常方便。使用混合液节省时间、简化反应体系构建流程,并通过消除移液失误和污染,增加可靠性,减少移液步骤和繁琐的 计算。只需加入引物和模板来制备最终扩增混合液。混合液可在2–8°C储存,可快速设置多重PCR检测。该试剂盒提供的精简的实验方案确保快速简单的 PCR设置。可在室温下设置反应,简单方便,易于使用。HotStarTaq DNA Polymerase可在95°C下孵育15分钟激活,因此可整合入各种现有的热循环流程中。

应用

QIAGEN Multiplex PCR Kit高度适用于各种多重应用,包括:

  • 转基因生物的分型和分析
  • 微卫星的扩增和分析
  • 细菌和病毒的分型和检测
  • 用于SNP分析的多个DNA扩增

产品详细信息

QIAGEN Multiplex PCR Kit (100)

206143

用于高特异性和灵敏度的多重PCR,无需优化

  • 无需优化步骤
  • 高特异性和高灵敏度,热启动
  • 高度适合用于各种类型的多重PCR应用
  • 使用简单,经济实惠

QIAGEN Multiplex PCR Kit提供方便的即用型预混液。QIAGEN Multiplex PCR Master Mix包含HotStarTaq DNA Polymerase和含有新型合成因子MP的独特PCR缓冲液。配合优化的盐浓度,MP因子可以促进引物与模板的特异性结合,使反应体系中所有的引物都 能有效延伸,无需额外优化。该试剂盒还包含新型的辅助剂Q-Solution,有助于实现“困难”模板(比如,GC含量高的模板)的高效扩增。

原理

QIAGEN Multiplex PCR Kit是首个专为多重PCR研发的试剂盒,有即用型混合液规格。QIAGEN Multiplex PCR Master Mix包含预优化浓度的HotStarTaq DNA Polymerase和MgCl2、dNTPs和新型的PCR缓冲液专为多重PCR研发。该试剂盒在首次尝试多重PCR即获得成功。由于试剂盒内独特的预优化的试剂无需优化反应条件(比如,引物浓度,Mg2+Taq DNA聚合酶)和循环参数。

HotStarTaq DNA Polymerase

HotStarTaq DNA Polymerase对 Taq DNA聚合酶进行改善,在室温下无聚合酶活性。这可以防止非特异性退火引物和在低温条件下的PCR设置和初始的PCR循环中形成引物二聚体。在95°C下,经15分钟诱导可激活HotStarTaq DNA Polymerase,可纳入各种现有的热循环程序中。

多重PCR缓冲液

这特殊的缓冲液包含优化的由K+和NH4+以及独特的PCR辅助剂、MP因子可增加模板引物的局部浓度。配合K+和其他阳离子、MP因子稳定特异性结合的引物,并能够用HotStarTaq DNA Polymerase进行高效的引物延伸(参见Stable and efficient primer annealing)。通过在每个PCR循环中高比率的特异性-非特异性引物结合,该新型的缓冲液维持每个PCR循环的特异性扩增。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该缓冲液能在更广泛的退火温度和Mg2+浓度下,提供严格的引物退火条件。通过改变退火温度或Mg2+浓度可优化PCR,因此通常将优化过程最小化或无需要优化。

Q-Solution

Q-Solution是一种新型的PCR辅助剂,有助于扩增由DNA融化特性修改的困难模板,同时配合HotStarTaq DNA Polymerase提供。该独特的试剂可优化高度二级结构或富含GC的模板所致的效果欠佳的PCR。不同于其他常用的PCR辅助剂如DMSO,Q-Solution只用于一种工作浓度,并保证无毒和PCR纯度好。

操作流程

QIAGEN Multiplex PCR Kit含有即用型、预优化的混合液,非常方便。使用混合液节省时间、简化反应体系构建流程,并通过消除移液失误和污染,增加可靠性,减少移液步骤和繁琐的 计算。只需加入引物和模板来制备最终扩增混合液。混合液可在2–8°C储存,可快速设置多重PCR检测。该试剂盒提供的精简的实验方案确保快速简单的 PCR设置。可在室温下设置反应,简单方便,易于使用。HotStarTaq DNA Polymerase可在95°C下孵育15分钟激活,因此可整合入各种现有的热循环流程中。

应用

QIAGEN Multiplex PCR Kit高度适用于各种多重应用,包括:

  • 转基因生物的分型和分析
  • 微卫星的扩增和分析
  • 细菌和病毒的分型和检测
  • 用于SNP分析的多个DNA扩增

mRNA转染QIAGEN 货号:301525

mRNA转染QIAGEN 货号:301525

mRNA转染QIAGEN

货  号:301525

产品规格:0.5ml

原  产  地:德国

参考价格:2100 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

mRNA转染QIAGEN  301525  2100元

TransMessenger Transfection Reagent高效的mRNA转染

    高效的转染
    严格的质量监控确保可靠的结果
    高效转染原代细胞

TransMessenger Transfection Reagent是即用型、脂质体试剂,用于真核细胞的RNA转染。使用RNA而非DNA转染的细胞为转染实验提供了新的可能性。使用TransMessenger Reagent成功转染的细胞株包含293T、Jurkat细胞和Vero细胞。该产品也可用于RNA转染人类原代成纤维细胞、大鼠心肌细胞和大鼠神经细胞。

原理

TransMessenger Transfection Reagent是首款专为用RNA转染细胞的试剂。该试剂由脂质体制备,与特定的RNA冷凝增强剂和优化的缓冲液一起使用。RNA分子通过增强剂冷凝,然后涂上TransMessenger Reagent有效地转移到真核细胞中。严格的质量监控用于测试无RNA酶活性、每批次的一致性和低内毒素水平(≤10 EU/ml)。我们严格的标准消除了试剂的变量,避免对RNA转染效率产生不利影响。
操作流程

TransMessenger Reagent为即用型试剂。为获得TransMessenger–RNA转染混合物,只需简单混合具增强剂R的RNA和Buffer EC-R,并在室温下孵育5分钟,然后加入TransMessenger Reagent再孵育5–10分钟。将其与培养液混合并直接加入细胞中。随后需要3小时的孵育,培养液发生改变,孵育细胞直到可用于分析应用。使用高纯度的RNA可获得最佳的转染结果,无DNA、蛋白质和其他污染物。推荐使用RNeasy和Oligotex mRNA Kits纯化RNA。
应用

RNA而非DNA转染的细胞为转染实验提供新的可能性。转染RNA序列的表达无需转录、不依赖于启动子。此外,蛋白表达通常会在RNA而非DNA转染后快速出现。使用TransMessenger Transfection Reagent的RNA转染可用于:

研究无法用质粒DNA高效转染的细胞
直接研究RNA功能

产品详细信息

mRNA转染QIAGEN  301525  2100元

TransMessenger Transfection Reagent高效的mRNA转染

    高效的转染
    严格的质量监控确保可靠的结果
    高效转染原代细胞

TransMessenger Transfection Reagent是即用型、脂质体试剂,用于真核细胞的RNA转染。使用RNA而非DNA转染的细胞为转染实验提供了新的可能性。使用TransMessenger Reagent成功转染的细胞株包含293T、Jurkat细胞和Vero细胞。该产品也可用于RNA转染人类原代成纤维细胞、大鼠心肌细胞和大鼠神经细胞。

原理

TransMessenger Transfection Reagent是首款专为用RNA转染细胞的试剂。该试剂由脂质体制备,与特定的RNA冷凝增强剂和优化的缓冲液一起使用。RNA分子通过增强剂冷凝,然后涂上TransMessenger Reagent有效地转移到真核细胞中。严格的质量监控用于测试无RNA酶活性、每批次的一致性和低内毒素水平(≤10 EU/ml)。我们严格的标准消除了试剂的变量,避免对RNA转染效率产生不利影响。
操作流程

TransMessenger Reagent为即用型试剂。为获得TransMessenger–RNA转染混合物,只需简单混合具增强剂R的RNA和Buffer EC-R,并在室温下孵育5分钟,然后加入TransMessenger Reagent再孵育5–10分钟。将其与培养液混合并直接加入细胞中。随后需要3小时的孵育,培养液发生改变,孵育细胞直到可用于分析应用。使用高纯度的RNA可获得最佳的转染结果,无DNA、蛋白质和其他污染物。推荐使用RNeasy和Oligotex mRNA Kits纯化RNA。
应用

RNA而非DNA转染的细胞为转染实验提供新的可能性。转染RNA序列的表达无需转录、不依赖于启动子。此外,蛋白表达通常会在RNA而非DNA转染后快速出现。使用TransMessenger Transfection Reagent的RNA转染可用于:

研究无法用质粒DNA高效转染的细胞
直接研究RNA功能

QIAGEN Multiplex PCR Plus Kit (100) 货号:206152

QIAGEN Multiplex PCR Plus Kit (100) 货号:206152

QIAGEN Multiplex PCR Plus Kit (100)

货  号:206152

产品规格:100

原  产  地:德国

参考价格:2960 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Multiplex PCR Plus Kit (100)

206152

用于成功的进行多重PCR反应

  • 成功的进行多重PCR反应,适用于高要求应用
  • 稳定、可靠的结果
  • 快速建立多重PCR反应体系,无需优化
  • 反应时间缩短,更加便利
QIAGEN Multiplex PCR Plus Kit专为便利、灵敏的多重PCR设计,无需优化。该试剂盒基于专有的多重PCR技术,能够在首次尝试即获得成功的结果,且比以往更快更便利。该试剂盒提 供便利的预混液。预混液独特的成分确保了反应的高特异性和高灵敏度。该预混液包含新型的缓冲液,专为多重PCR研发。该试剂盒还提供新型的辅助剂Q- Solution,有助于扩增难以扩增的片段,如富含GC的区域或具复杂二级结构的模板。该试剂盒还提供CoralLoad Dye,确保DNA迁移的操作便利且可视。

原理

QIAGEN Multiplex PCR Plus Kit基于QIAGEN专有的多重PCR技术,含有即用型混合液。该试剂盒所有的成分专为提供最易操作和最大速度研发,提供一致可靠的结果(参见下表)。Multiplex PCR Master Mix包含优化的HotStarTaq Plus DNA Polymerase和MgCl2浓度,以及dNTP和新型的PCR缓冲液。

多重PCR成功的关键因素,用于各种应用的一致可靠的结果

Multiplex PCR为研究者节省时间和成本用于进行大量的PCR反应,广泛应用于基因分型以及在研究、法医和分子检测实验室的DNA或cDNA检测应用。应用范围包括 分型和转基因生物和病原体的分析、微卫星的扩增分析、检测SNP突变区域,以及宏基因组学研究。然而,多重PCR技术要求严格。PCR优化是多重PCR成 功与否的关键因素。多重PCR中不同引物对不同杂交动力学可能导致如下问题,如扩增偏向性。能高效率结合的引物,更多地利用扩增反应元件,从而降低了其他 PCR产物的产量。由于更大的引物量,因此存在更大的形成引物二聚体和非特异性启动的风险。不解决这些风险导致低灵敏度、非特异性扩增和选择靶位的偏向性 扩增,因此得到不一致的不令人满意的结果。共格这些瓶颈需要繁琐耗时的优化步骤,导致成本的增加。QIAGEN Multiplex PCR Plus Kit消除了这些风险,在首次尝试时就运行顺利。Multiplex PCR Master Mix独特的组成确保了高特异性和灵敏度的扩增反应,甚至是难扩增的靶位。该试剂盒能快速成功地建立所有多重PCR检测,有效的节省时间和成本(参见Successful multiplex PCR without the need for optimization)。

HotStarTaq Plus DNA Polymerase

HotStarTaq Plus DNA PolymeraseTaq DNA聚合酶的优化形式,在室温下无聚合酶活性。这能防止在PCR设置和PCR初始循环过程中,低温下非特异性退货引物的延伸和引物二聚体的形成。无需优 化反应条件。在室温下可方便设置多重检测。HotStarTaq DNA Polymerase在95°C下孵育5分钟激活,可整合入现有的各种热循环程序中。

多重PCR缓冲液

这特殊的缓冲液包含优化的混合物由K+和NH4+以及独特的PCR辅助剂、MP因子组成可增加模板引物的局部浓度(参见Stable and efficient annealing)。将非特异性退火最小化并成功地平行扩增所有靶位,甚至只有非常少量的模板。配合K+和其他阳离子、MP因子稳定特异性结合的引物,并能够用HotStarTaq Plus DNA Polymerase进行高效的引物延伸。通过在每个PCR循环中高比率的特异性-非特异性引物结合,该新型的缓冲液维持每个PCR循环的特异性扩增。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该缓冲液能在更广泛的退火温度和Mg2+浓度下,提供严格的引物退火条件。通过改变退火温度或Mg2+浓度可优化PCR,因此通常将优化过程最小化或无需要优化。

该试剂盒包含独特的辅助剂Q-Solution,促进难扩增的靶位扩增如富含GC的区域或具有复杂二级结构的模板。该试剂盒还含有高度灵活的CoralLoad Dye,通过提高移液器的可视性和后续的凝胶上样以及DNA迁移的可视性,来进一步提高操作的方便性(参见Easy PCR setup and convenient DNA visualization)。

QIAGEN Multiplex PCR Plus Kit的优点
试剂盒组分 优点
HotStarTaq Plus DNA Polymerase 高特异性和灵敏度扩增
室温设置
5分钟激活时间
Multiplex PCR Plus Buffer with Factor MP 平行扩增所有靶位
无需优化PCR参数
单一的用于所有多重检测的实验方案
快速的操作流程
CoralLoad Dye 移液过程中简单的可视化
可即可凝胶上样
DNA迁移的可视化
Q-Solution 扩增困难靶位

操作流程

QIAGEN Multiplex PCR Plus Kit能够在首次尝试就可成功进行多重PCR。

简单快速的下游分析

QIAGEN Multiplex PCR Plus Kit使用多种方法得到的多重PCR产物的下游分析简单快速。无论分析是否使用琼脂糖凝胶电泳,毛细管电泳或是否使用QIAxcel Advanced System,所有阳离子可方便的可视化,单独的片段也可有效区别。

便利的试剂盒规格

QIAGEN Multiplex PCR Plus Kit提供更方便的即用型预优化混合液。使用混合物节省时间、简化反应设置操作,并通过消除众多移液失误和污染的可能性来增加可靠性,将移液步骤最小化并 去除繁琐的计算。只需将引物和模板加入来制备最终扩增混合物。混合液可在2–8°C下储存,可更快速的设置多重PCR检测。可在室温下设置反应,操作简 便,易于使用。HotStarTaq Plus DNA Polymerase在95°C经5分钟孵育激活,可整合入现有的热循环程序中。试剂盒中包含的CoralLoad Dye便于使用,提高PCR设置中移液的可视化和凝胶上DNA迁移的可视化。

应用
QIAGEN Multiplex PCR Plus Kit能成功应用于多种应用,从转基因生物分型、病原体检测到癌症研究、宏基因组学和基因表达分析。

产品详细信息

QIAGEN Multiplex PCR Plus Kit (100)

206152

用于成功的进行多重PCR反应

  • 成功的进行多重PCR反应,适用于高要求应用
  • 稳定、可靠的结果
  • 快速建立多重PCR反应体系,无需优化
  • 反应时间缩短,更加便利
QIAGEN Multiplex PCR Plus Kit专为便利、灵敏的多重PCR设计,无需优化。该试剂盒基于专有的多重PCR技术,能够在首次尝试即获得成功的结果,且比以往更快更便利。该试剂盒提 供便利的预混液。预混液独特的成分确保了反应的高特异性和高灵敏度。该预混液包含新型的缓冲液,专为多重PCR研发。该试剂盒还提供新型的辅助剂Q- Solution,有助于扩增难以扩增的片段,如富含GC的区域或具复杂二级结构的模板。该试剂盒还提供CoralLoad Dye,确保DNA迁移的操作便利且可视。

原理

QIAGEN Multiplex PCR Plus Kit基于QIAGEN专有的多重PCR技术,含有即用型混合液。该试剂盒所有的成分专为提供最易操作和最大速度研发,提供一致可靠的结果(参见下表)。Multiplex PCR Master Mix包含优化的HotStarTaq Plus DNA Polymerase和MgCl2浓度,以及dNTP和新型的PCR缓冲液。

多重PCR成功的关键因素,用于各种应用的一致可靠的结果

Multiplex PCR为研究者节省时间和成本用于进行大量的PCR反应,广泛应用于基因分型以及在研究、法医和分子检测实验室的DNA或cDNA检测应用。应用范围包括 分型和转基因生物和病原体的分析、微卫星的扩增分析、检测SNP突变区域,以及宏基因组学研究。然而,多重PCR技术要求严格。PCR优化是多重PCR成 功与否的关键因素。多重PCR中不同引物对不同杂交动力学可能导致如下问题,如扩增偏向性。能高效率结合的引物,更多地利用扩增反应元件,从而降低了其他 PCR产物的产量。由于更大的引物量,因此存在更大的形成引物二聚体和非特异性启动的风险。不解决这些风险导致低灵敏度、非特异性扩增和选择靶位的偏向性 扩增,因此得到不一致的不令人满意的结果。共格这些瓶颈需要繁琐耗时的优化步骤,导致成本的增加。QIAGEN Multiplex PCR Plus Kit消除了这些风险,在首次尝试时就运行顺利。Multiplex PCR Master Mix独特的组成确保了高特异性和灵敏度的扩增反应,甚至是难扩增的靶位。该试剂盒能快速成功地建立所有多重PCR检测,有效的节省时间和成本(参见Successful multiplex PCR without the need for optimization)。

HotStarTaq Plus DNA Polymerase

HotStarTaq Plus DNA PolymeraseTaq DNA聚合酶的优化形式,在室温下无聚合酶活性。这能防止在PCR设置和PCR初始循环过程中,低温下非特异性退货引物的延伸和引物二聚体的形成。无需优 化反应条件。在室温下可方便设置多重检测。HotStarTaq DNA Polymerase在95°C下孵育5分钟激活,可整合入现有的各种热循环程序中。

多重PCR缓冲液

这特殊的缓冲液包含优化的混合物由K+和NH4+以及独特的PCR辅助剂、MP因子组成可增加模板引物的局部浓度(参见Stable and efficient annealing)。将非特异性退火最小化并成功地平行扩增所有靶位,甚至只有非常少量的模板。配合K+和其他阳离子、MP因子稳定特异性结合的引物,并能够用HotStarTaq Plus DNA Polymerase进行高效的引物延伸。通过在每个PCR循环中高比率的特异性-非特异性引物结合,该新型的缓冲液维持每个PCR循环的特异性扩增。由于KCl和(NH4)2SO4独特的平衡结合,相比传统的PCR缓冲液,该缓冲液能在更广泛的退火温度和Mg2+浓度下,提供严格的引物退火条件。通过改变退火温度或Mg2+浓度可优化PCR,因此通常将优化过程最小化或无需要优化。

该试剂盒包含独特的辅助剂Q-Solution,促进难扩增的靶位扩增如富含GC的区域或具有复杂二级结构的模板。该试剂盒还含有高度灵活的CoralLoad Dye,通过提高移液器的可视性和后续的凝胶上样以及DNA迁移的可视性,来进一步提高操作的方便性(参见Easy PCR setup and convenient DNA visualization)。

QIAGEN Multiplex PCR Plus Kit的优点
试剂盒组分 优点
HotStarTaq Plus DNA Polymerase 高特异性和灵敏度扩增
室温设置
5分钟激活时间
Multiplex PCR Plus Buffer with Factor MP 平行扩增所有靶位
无需优化PCR参数
单一的用于所有多重检测的实验方案
快速的操作流程
CoralLoad Dye 移液过程中简单的可视化
可即可凝胶上样
DNA迁移的可视化
Q-Solution 扩增困难靶位

操作流程

QIAGEN Multiplex PCR Plus Kit能够在首次尝试就可成功进行多重PCR。

简单快速的下游分析

QIAGEN Multiplex PCR Plus Kit使用多种方法得到的多重PCR产物的下游分析简单快速。无论分析是否使用琼脂糖凝胶电泳,毛细管电泳或是否使用QIAxcel Advanced System,所有阳离子可方便的可视化,单独的片段也可有效区别。

便利的试剂盒规格

QIAGEN Multiplex PCR Plus Kit提供更方便的即用型预优化混合液。使用混合物节省时间、简化反应设置操作,并通过消除众多移液失误和污染的可能性来增加可靠性,将移液步骤最小化并 去除繁琐的计算。只需将引物和模板加入来制备最终扩增混合物。混合液可在2–8°C下储存,可更快速的设置多重PCR检测。可在室温下设置反应,操作简 便,易于使用。HotStarTaq Plus DNA Polymerase在95°C经5分钟孵育激活,可整合入现有的热循环程序中。试剂盒中包含的CoralLoad Dye便于使用,提高PCR设置中移液的可视化和凝胶上DNA迁移的可视化。

应用
QIAGEN Multiplex PCR Plus Kit能成功应用于多种应用,从转基因生物分型、病原体检测到癌症研究、宏基因组学和基因表达分析。

QIAGEN Plasmid Mini Kit (25) 货号:12123

QIAGEN Plasmid Mini Kit (25) 货号:12123

QIAGEN Plasmid Mini Kit (25)

货  号:12123

产品规格:25

原  产  地:德国

参考价格:2200 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Plasmid Mini Kit (25)

12123

纯化至多20 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多20 μg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性,使纯化的DNA的 纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套 QIAGEN质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、 代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,通过离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA适合多种应用,如:

转染
克隆
PCR
体外转录

产品详细信息

QIAGEN Plasmid Mini Kit (25)

12123

纯化至多20 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多20 μg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性,使纯化的DNA的 纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套 QIAGEN质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、 代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,通过离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA适合多种应用,如:

转染
克隆
PCR
体外转录

QIAGEN Plasmid Mini Kit (100) 货号:12125

QIAGEN Plasmid Mini Kit (100) 货号:12125

QIAGEN Plasmid Mini Kit (100)

货  号:12125

产品规格:100

原  产  地:德国

参考价格:7010 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Plasmid Mini Kit (100)

12125

纯化至多20 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多20 μg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性,使纯化的DNA的 纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套 QIAGEN质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、 代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,通过离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA适合多种应用,如:

转染
克隆
PCR
体外转录

产品详细信息

QIAGEN Plasmid Mini Kit (100)

12125

纯化至多20 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多20 μg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性,使纯化的DNA的 纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套 QIAGEN质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、 代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,通过离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA适合多种应用,如:

转染
克隆
PCR
体外转录

QIAGEN Plasmid Maxi Kit (10) 货号:12162

QIAGEN Plasmid Maxi Kit (10) 货号:12162

QIAGEN Plasmid Maxi Kit (10)

货  号:12162

产品规格:10

原  产  地:德国

参考价格:2600 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Plasmid Maxi Kit (10)

12162

纯化至多500 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级纯质粒DNA,可制备至多500 μg的质粒DNA。离心获得澄清的裂解液和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换柱专为纯化核酸设计。它独特的分离特性,使纯化的DNA的纯 度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套QIAGEN 质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程
使用QIAGEN Plasmid Kits直接离心澄清细菌裂解液。澄清后的裂解液上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、代 谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯化质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA高度适用于多种应用,如:

转染
克隆
PCR
体外转录

产品详细信息

QIAGEN Plasmid Maxi Kit (10)

12162

纯化至多500 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级纯质粒DNA,可制备至多500 μg的质粒DNA。离心获得澄清的裂解液和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换柱专为纯化核酸设计。它独特的分离特性,使纯化的DNA的纯 度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套QIAGEN 质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程
使用QIAGEN Plasmid Kits直接离心澄清细菌裂解液。澄清后的裂解液上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、代 谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯化质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA高度适用于多种应用,如:

转染
克隆
PCR
体外转录

QIAGEN Plasmid Midi Kit (25) 货号:12143

QIAGEN Plasmid Midi Kit (25) 货号:12143

QIAGEN Plasmid Midi Kit (25)

货  号:12143

产品规格:25

原  产  地:德国

参考价格:3010 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

QIAGEN Plasmid Midi Kit (25)

12143

纯化至多100 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits通过重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多100 μg质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性,使纯化的DNA的 纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套 QIAGEN质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、 代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,通过离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA适合多种应用,如:

转染
克隆
PCR
体外转录

产品详细信息

QIAGEN Plasmid Midi Kit (25)

12143

纯化至多100 µg转染级质粒或柯斯质粒DNA

  • 纯化得到相当于2次CsCl梯度离心得到的DNA纯度
  • 高产量质粒DNA
  • 经济的制备方法
  • 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits通过重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多100 μg质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

原理

QIAGEN-tips内独特的阴离子交换树脂专为纯化核酸设计。它独特的分离特性,使纯化的DNA的 纯度相当于连续2次CsCl梯度离心得到的DNA纯度。预装的QIAGEN-tips利用重力流原理工作,缩短了纯化质粒的手动操作时间。整套 QIAGEN质粒纯化体系避免使用苯酚、氯仿、溴化乙锭和CsCl等有毒物质,减小对使用者和环境的危害。

操作流程

使用QIAGEN Plasmid Kits,细菌裂解物通过离心澄清。澄清后的裂解物上样到阴离子交换柱,质粒DNA在适当的低盐条件和pH条件下与树脂进行选择性结合。RNA、蛋白质、 代谢产物和和其它低分子量杂质用中盐洗涤液去除,用高盐缓冲液洗脱纯质粒DNA(参见QIAGEN Plasmid Kit procedures)。纯化得到的DNA用异丙醇沉淀浓缩和脱盐后,通过离心收集。

应用

QIAGEN Plasmid Kits纯化得到的质粒DNA适合多种应用,如:

转染
克隆
PCR
体外转录