Qubit dsDNA HS 定量检测试剂盒

产品介绍

Qubit dsDNA HS分析试剂盒(Qubit dsDNA HS Assay Kit) 专门设计用于在Qubit 荧光仪, 但也可以在任何荧光计或荧光酶标仪中使用。该试剂盒对双链DNA(dsDNA)有高度的选择性，不会定量RNA，可以定量浓度为10 pg/µl 到100 ng/µl的样品。试剂盒提供了浓缩的分析试剂，稀释缓冲液和预制的DNA标准。您只需用提供的缓冲液稀释试剂，添加样品(体积在 1 µL 到 20 µL 范围内), 然后使用Qubit荧光仪读取浓度。该试剂盒对于常见污染物，如盐，游离的核苷酸，溶剂，去污剂，蛋白质的耐受性极好。

HS (High Sensitivity)：高灵敏度

搭配仪器

对于1-20个样品：Qubit dsDNA HS定量试剂盒和Qubit荧光仪一起使用

对于20-2000个样品：Quant-iT dsDNA HS定量试剂盒和酶标仪（microplate reader）一起使用

注意事项

1.所有Qubit定量试剂盒都可以与Qubit 1.0，Qubit 2.0，Qubit 3和Qubit 4荧光计一起使用。

2.需要500μL薄壁PCR管，但不包括在内。

3.也可作为1X dsDNA定量试剂盒，配好的试剂可作为即用型溶液稳定长达一年。

产品特点

核心范围（高可信度）：1 ng / mL至500 ng / mL

扩展范围（中等可信度）：0.5 ng / mL至1 ng / mL和500 ng / mL至600 ng / mL

用于定量PCR产物、病毒DNA和亚克隆（subcloning）样品

在RNA，盐，溶剂，蛋白质和游离核苷酸的存在下是准确的

产品参数

Ex(nm) 488        Em(nm) 520

自备材料

用于混合Qubit工作溶液的塑料容器（一次性）

Qubit  Assay Tubes分析试管（500管）或Axygen PCR-05-C管（VWR）

Product description

Qubit dsDNA HS Assay Kit (Qubit dsDNA HS Assay Kit) is specially designed to be used in Qubit Fluorometer, but it can also be used in any fluorometer or fluorescence microplate reader. The kit is highly selective for double-stranded DNA (dsDNA), does not quantify RNA, and can quantify samples with concentrations ranging from 10 pg/µl to 100 ng/µl. The kit provides concentrated analysis reagents, dilution buffer and pre-made DNA standards. All you need to do is to dilute the reagent with the provided buffer, add the sample (volume in the range of 1 µL to 20 µL), and then use the Qubit Fluorometer to read the concentration. The kit has excellent tolerance to common contaminants such as salts, free nucleotides, solvents, detergents, and proteins.

HS (High Sensitivity): High sensitivity

Matching instrument

For 1-20 samples: Qubit dsDNA HS Quantitative Kit and Qubit Fluorometer are used together

For 20-2000 samples: Quant-iT dsDNA HS quantitative kit and microplate reader are used together

Precautions

1. All Qubit quantitative kits can be used with Qubit 1.0, Qubit 2.0, Qubit 3 and Qubit 4 fluorometers.

2. A 500μL thin-walled PCR tube is required, but not included.

3. It can also be used as a 1X dsDNA quantitative kit. The prepared reagents can be used as a ready-to-use solution that is stable for up to one year.

Features

Core range (high confidence): 1 ng / mL to 500 ng / mL

Extended range (medium confidence): 0.5 ng/mL to 1 ng/mL and 500 ng/mL to 600 ng/mL

Used for quantitative PCR products, viral DNA and subcloning samples

It is accurate in the presence of RNA, salt, solvent, protein and free nucleotide

Product parameter

Ex(nm) 488    Em(nm) 520

Bring your own materials

Plastic container for mixing Qubit working solution (disposable)

Qubit Assay Tubes (500 tubes) or Axygen PCR-05-C tubes (VWR)