Chemical Phosphorylation Reagent I (CPR I), ,Chemical Phosphorylation Reagent I (CPR I),

Chemical Phosphorylation Reagent I (CPR I)

有货

Chemical Phosphorylation Reagent I (CPR I), ,Chemical Phosphorylation Reagent I (CPR I),

品牌:Jinpan
Chemical Phosphorylation Reagent I (CPR I)

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
C273246-100mg 100mg 期货 Chemical Phosphorylation Reagent I (CPR I), ,Chemical Phosphorylation Reagent I (CPR I),  

基本信息

产品名称 Chemical Phosphorylation Reagent I (CPR I)
英文名称 Chemical Phosphorylation Reagent I (CPR I)
运输条件 超低温冰袋运输

一般描述

Product description

Solvent:MeCN,DMSO

MW : 656.77
Chemical Phosphorylation Reagent I (CPR I), ,Chemical Phosphorylation Reagent I (CPR I),

Features and Biological Applications

This reagent can be used to create the 3′-phosphate by adding it as the first addition to the support in Labeling Oligonucleotides with Fluorescent Dyes. After ammonia deprotection the oligo will have the 3′-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.

References

1.  Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD. (2006) Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate. J Am Chem Soc, 128, 885.

2.  Ohkubo A, Ezawa Y, Seio K, Sekine M. (2004) O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method. J Am Chem Soc, 126, 10884.

3.  Tsuruoka H, Shohda K, Wada T, Sekine M. (2000) Synthesis and conformational properties of oligonucleotides incorporating 2′-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates. J Org Chem, 65, 7479.

4. Olejnik J, Krzymanska-Olejnik E, Rothschild KJ. (1996) Photocleavable biotin phosphoramidite for 5′-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides. Nucleic Acids Res, 24, 361.

5.  Mora N, Lacombe JM, Pavia AA. (1995) A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase. Int J Pept Protein Res, 45, 53.

6.  Boumendjel A, Miller SP. (1994) Synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate. J Lipid Res, 35, 2305.

7. Kitas E, Kung E, Bannwarth W. (1994) Chemical synthesis of O-thiophosphotyrosyl peptides. Int J Pept Protein Res, 43, 146.

8.  Tegge W, Ballou CE. (1992) Syntheses of D-myo-inositol 1,4,5-trisphosphate affinity ligands. Carbohydr Res, 230, 63.

9.  Perich JW, Reynolds EC. (1991) Fmoc/solid-phase synthesis of Tyr(P)-containing peptides through t-butyl phosphate protection. Int J Pept Protein Res, 37, 572.

10. Lacombe JM, Andriamanampisoa F, Pavia AA. (1990) Solid-phase synthesis of peptides containing phosphoserine using phosphate tert.-butyl protecting group. Int J Pept Protein Res, 36, 275.

Product description

Solvent:MeCN,DMSO

MW : 656.77
Chemical Phosphorylation Reagent I (CPR I), ,Chemical Phosphorylation Reagent I (CPR I),

Features and Biological Applications

This reagent can be used to create the 3′-phosphate by adding it as the first addition to the support in Labeling Oligonucleotides with Fluorescent Dyes. After ammonia deprotection the oligo will have the 3′-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.

References

1.  Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD. (2006) Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate. J Am Chem Soc, 128, 885.

2.  Ohkubo A, Ezawa Y, Seio K, Sekine M. (2004) O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method. J Am Chem Soc, 126, 10884.

3.  Tsuruoka H, Shohda K, Wada T, Sekine M. (2000) Synthesis and conformational properties of oligonucleotides incorporating 2′-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates. J Org Chem, 65, 7479.

4. Olejnik J, Krzymanska-Olejnik E, Rothschild KJ. (1996) Photocleavable biotin phosphoramidite for 5′-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides. Nucleic Acids Res, 24, 361.

5.  Mora N, Lacombe JM, Pavia AA. (1995) A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase. Int J Pept Protein Res, 45, 53.

6.  Boumendjel A, Miller SP. (1994) Synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate. J Lipid Res, 35, 2305.

7. Kitas E, Kung E, Bannwarth W. (1994) Chemical synthesis of O-thiophosphotyrosyl peptides. Int J Pept Protein Res, 43, 146.

8.  Tegge W, Ballou CE. (1992) Syntheses of D-myo-inositol 1,4,5-trisphosphate affinity ligands. Carbohydr Res, 230, 63.

9.  Perich JW, Reynolds EC. (1991) Fmoc/solid-phase synthesis of Tyr(P)-containing peptides through t-butyl phosphate protection. Int J Pept Protein Res, 37, 572.

10. Lacombe JM, Andriamanampisoa F, Pavia AA. (1990) Solid-phase synthesis of peptides containing phosphoserine using phosphate tert.-butyl protecting group. Int J Pept Protein Res, 36, 275.

相关属性

敏感性 对光和湿度敏感
储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Chemical Phosphorylation Reagent II (CPR II), ,Chemical Phosphorylation Reagent II (CPR II),<p><br></p>

Chemical Phosphorylation Reagent II (CPR II)

有货

Chemical Phosphorylation Reagent II (CPR II), ,Chemical Phosphorylation Reagent II (CPR II),&lt;p&gt;&lt;br&gt;&lt;/p&gt;

品牌:Jinpan
Chemical Phosphorylation Reagent II (CPR II)

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
C273361-100mg 100mg 期货 Chemical Phosphorylation Reagent II (CPR II), ,Chemical Phosphorylation Reagent II (CPR II),&lt;p&gt;&lt;br&gt;&lt;/p&gt;  

基本信息

产品名称 Chemical Phosphorylation Reagent II (CPR II)
英文名称 Chemical Phosphorylation Reagent II (CPR II)
运输条件 超低温冰袋运输

一般描述

Product description

Solvent:MeCN,DMSO

MW : 722.8
Chemical Phosphorylation Reagent II (CPR II), ,Chemical Phosphorylation Reagent II (CPR II),&lt;p&gt;&lt;br&gt;&lt;/p&gt;

Features and Biological Applications

This Chemical Phosphorylation Reagent (CPR II) contains a DMT group that can be left on the oligonucleotide and used for rapid purification of oligonucleotide 5′-phosphates by the popular DMTr-on technique, which employs disposable RP cartridges or “Trityl-on” RP HPLC purification. The DMTr group is removed with aqueous acid (e.g., 2%TFA in the case of Cartridge Purification) and the remaining linker is then eliminated after brief treatment with aqueous ammonium hydroxide (12 -15% ammonium hydroxide at room temperature for 15 minutes) to yield the 5′-phosphate.

References

1. Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD. (2006) Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate. J Am Chem Soc, 128, 885.

2. Ohkubo A, Ezawa Y, Seio K, Sekine M. (2004) O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method. J Am Chem Soc, 126, 10884.

3. Tsuruoka H, Shohda K, Wada T, Sekine M. (2000) Synthesis and conformational properties of oligonucleotides incorporating 2′-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates. J Org Chem, 65, 7479.

4. Olejnik J, Krzymanska-Olejnik E, Rothschild KJ. (1996) Photocleavable biotin phosphoramidite for 5′-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides. Nucleic Acids Res, 24, 361.

5. Mora N, Lacombe JM, Pavia AA. (1995) A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase. Int J Pept Protein Res, 45, 53.

6.  Boumendjel A, Miller SP. (1994) Synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate. J Lipid Res, 35, 2305.

7. Kitas E, Kung E, Bannwarth W. (1994) Chemical synthesis of O-thiophosphotyrosyl peptides. Int J Pept Protein Res, 43, 146.

8. Tegge W, Ballou CE. (1992) Syntheses of D-myo-inositol 1,4,5-trisphosphate affinity ligands. Carbohydr Res, 230, 63.

9. Perich JW, Reynolds EC. (1991) Fmoc/solid-phase synthesis of Tyr(P)-containing peptides through t-butyl phosphate protection. Int J Pept Protein Res, 37, 572.

10. Lacombe JM, Andriamanampisoa F, Pavia AA. (1990) Solid-phase synthesis of peptides containing phosphoserine using phosphate tert.-butyl protecting group. Int J Pept Protein Res, 36, 275.

Product description

Solvent:MeCN,DMSO

MW : 722.8
Chemical Phosphorylation Reagent II (CPR II), ,Chemical Phosphorylation Reagent II (CPR II),&lt;p&gt;&lt;br&gt;&lt;/p&gt;

Features and Biological Applications

This Chemical Phosphorylation Reagent (CPR II) contains a DMT group that can be left on the oligonucleotide and used for rapid purification of oligonucleotide 5′-phosphates by the popular DMTr-on technique, which employs disposable RP cartridges or “Trityl-on” RP HPLC purification. The DMTr group is removed with aqueous acid (e.g., 2%TFA in the case of Cartridge Purification) and the remaining linker is then eliminated after brief treatment with aqueous ammonium hydroxide (12 -15% ammonium hydroxide at room temperature for 15 minutes) to yield the 5′-phosphate.

References

1. Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD. (2006) Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate. J Am Chem Soc, 128, 885.

2. Ohkubo A, Ezawa Y, Seio K, Sekine M. (2004) O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method. J Am Chem Soc, 126, 10884.

3. Tsuruoka H, Shohda K, Wada T, Sekine M. (2000) Synthesis and conformational properties of oligonucleotides incorporating 2′-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates. J Org Chem, 65, 7479.

4. Olejnik J, Krzymanska-Olejnik E, Rothschild KJ. (1996) Photocleavable biotin phosphoramidite for 5′-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides. Nucleic Acids Res, 24, 361.

5. Mora N, Lacombe JM, Pavia AA. (1995) A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase. Int J Pept Protein Res, 45, 53.

6.  Boumendjel A, Miller SP. (1994) Synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate. J Lipid Res, 35, 2305.

7. Kitas E, Kung E, Bannwarth W. (1994) Chemical synthesis of O-thiophosphotyrosyl peptides. Int J Pept Protein Res, 43, 146.

8. Tegge W, Ballou CE. (1992) Syntheses of D-myo-inositol 1,4,5-trisphosphate affinity ligands. Carbohydr Res, 230, 63.

9. Perich JW, Reynolds EC. (1991) Fmoc/solid-phase synthesis of Tyr(P)-containing peptides through t-butyl phosphate protection. Int J Pept Protein Res, 37, 572.

10. Lacombe JM, Andriamanampisoa F, Pavia AA. (1990) Solid-phase synthesis of peptides containing phosphoserine using phosphate tert.-butyl protecting group. Int J Pept Protein Res, 36, 275.

相关属性

敏感性 对光和湿度敏感
储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Binding Reagent (VXB) (350 ml) 货号:950233

Binding Reagent (VXB) (350 ml) 货号:950233

Binding Reagent (VXB) (350 ml)

货  号:950233

产品规格:350ml

原  产  地:德国

参考价格:2140 (参考价格,以实际价格为准)

优惠价格:

产品详细信息

Binding Reagent (VXB) (350 ml)

950233

从各种类型样本中经济、高通量的纯化核酸

  • 经优化的操作流程,即使难处理样品也能获得高品质结果
  • 交叉污染的风险最小化,十分可靠
  • 方便、易于使用、占地面积小
  • 快速、自动的操作流程让你有更多时间用于其它任务
  • 运行前后完整的文档记录

QIAxtractor表现卓越,高度可靠,每次可从8–96个样品中纯化高品质核酸。创新的仪器特性确保可靠的纯化,将交叉污染的风险降至最低。QIAxtractor是完全整合的解决方案,提供试剂、耗材以及从各种样品中纯化核酸的优化操作流程。

原理

QIAxtractor的自动纯化过程快速,并十分经济;在96分钟内即可从96个样本中纯化获得高品质核酸。QIAxtractor的优化试 剂可从不同的起始样本中纯化核酸,包括液体样本和组织。QIAxtractor极易使用,其配套的软件十分直观,您只需点击鼠标即可开始操作。

QIAxtractor具有新颖紧凑的设计,占地非常小。紧凑的设计使仪器易于移动,以适应样品制备要求。为了增加灵活性,与紧凑的设计相配套,QIAxtractor配有笔记本电脑。

先进的仪器安全性,包括半透明罩,有助于保护珍稀的样本不受环境污染。HEPA过滤器能维持在半透明罩下工作台上洁净的正向气压。紫外线可有效净化工作台,有助于防止交叉感染。

另有两款专有的QIAxtractor的产品线。QIAxtractor DX Reagents和Plasticware适用于纯化基因组DNA;QIAxtractor VX Reagents和Plasticware适用于纯化病毒核酸。试剂和耗材包含所有您制备样本所需的材料,包括纯化试剂和耗材,乙醇和一次性过滤头。这能 最大限度地给予用户方便,让您的实验快速进行。

产品
  • 产品目录
  • 自动化解决方案
    142
  • 样本破碎
    5
  • 样本制备
    33
  • 反应体系构建
    5
  • 检测与分析
    14
  • 焦磷酸测序
    19
  • 附件
    71
  • 服务与支持
    3
  • 分子诊断
  • 二代测序
  • 生物信息学
  • 生命科学领域研究
  • 人类身份认定与法医学检测
  • 动物和兽医学
  • 基因或通路特异性产品搜索
Quick order

Add to cart

订阅电子通讯

了解更多 订阅

QIAxtractor

打印 分享 加入收藏列表

Binding Reagent (VXB) (350 ml) 货号:950233

从各种类型样本中经济、高通量的纯化核酸

  • 经优化的操作流程,即使难处理样品也能获得高品质结果
  • 交叉污染的风险最小化,十分可靠
  • 方便、易于使用、占地面积小
  • 快速、自动的操作流程让你有更多时间用于其它任务
  • 运行前后完整的文档记录

QIAxtractor表现卓越,高度可靠,每次可从8–96个样品中纯化高品质核酸。创新的仪器特性确保可靠的纯化,将交叉污染的风险降至最低。QIAxtractor是完全整合的解决方案,提供试剂、耗材以及从各种样品中纯化核酸的优化操作流程。

  •  订购信息
  •  详细信息
  •  技术参数
  •  相关资料

性能
使用QIAxtractor纯化得到的核酸在灵敏的应用中有优越表现。通过QIAxtractor裂解样本,并且样本可以在仪器上,或脱离仪器进行孵育。特别设计的新型过滤头确保可靠的性能,有助于降低交叉污染的风险(参见High performance and reliability in sensitive applications)。

原理

QIAxtractor的自动纯化过程快速,并十分经济;在96分钟内即可从96个样本中纯化获得高品质核酸。QIAxtractor的优化试 剂可从不同的起始样本中纯化核酸,包括液体样本和组织。QIAxtractor极易使用,其配套的软件十分直观,您只需点击鼠标即可开始操作。

QIAxtractor具有新颖紧凑的设计,占地非常小。紧凑的设计使仪器易于移动,以适应样品制备要求。为了增加灵活性,与紧凑的设计相配套,QIAxtractor配有笔记本电脑。

先进的仪器安全性,包括半透明罩,有助于保护珍稀的样本不受环境污染。HEPA过滤器能维持在半透明罩下工作台上洁净的正向气压。紫外线可有效净化工作台,有助于防止交叉感染。

另有两款专有的QIAxtractor的产品线。QIAxtractor DX Reagents和Plasticware适用于纯化基因组DNA;QIAxtractor VX Reagents和Plasticware适用于纯化病毒核酸。试剂和耗材包含所有您制备样本所需的材料,包括纯化试剂和耗材,乙醇和一次性过滤头。这能 最大限度地给予用户方便,让您的实验快速进行。

操作流程

经优化的实验方案能处理液体样本和组织(参见下表)。只需选择最适合您起始样本的实验方案并启动运行。利用方便的软件向导,只需点击几下鼠标,即可开始操作。每次运行前和运行后的报告可提供完整的文档。

经优化的实验方案适用于各种样本类型

实验方案 裂解物 样本类型
液体 Buffer DXL 体液(血液、血浆、血清、尿液、呼吸道样本)和细胞悬浮液(白膜、细胞培养物)
组织 Buffer DXT 组织如活组织检查、鼠尾、对虾组织、鸟羽毛的羽髓、大脑、肝脏、肌肉等组织

QIAxtractor 可与您的工作流程无缝接合,与QIAGEN分析技术兼容。每次可自动纯化8至96个样品,解放您的双手,让您有更多时间处理其他事务。产物核酸可收集到 96个特别设计的管中,实现与您工作流程的接合。装有产物核酸离心管的洗脱板可转移到QIAgility上,自动构建PCR反应体系。可在Rotor- Gene Q实时荧光定量PCR分析仪上进行real-time PCR或RT-PCR,而终点式PCR可在QIAxcel上完成。

应用
纯化得到的核酸特别适用于敏感的应用,如:

  • 基因分型 
  • 生物标志物的测序研究
  • 基因组学研究
  • 生物医学研究

产品详细信息

Binding Reagent (VXB) (350 ml)

950233

从各种类型样本中经济、高通量的纯化核酸

  • 经优化的操作流程,即使难处理样品也能获得高品质结果
  • 交叉污染的风险最小化,十分可靠
  • 方便、易于使用、占地面积小
  • 快速、自动的操作流程让你有更多时间用于其它任务
  • 运行前后完整的文档记录

QIAxtractor表现卓越,高度可靠,每次可从8–96个样品中纯化高品质核酸。创新的仪器特性确保可靠的纯化,将交叉污染的风险降至最低。QIAxtractor是完全整合的解决方案,提供试剂、耗材以及从各种样品中纯化核酸的优化操作流程。

原理

QIAxtractor的自动纯化过程快速,并十分经济;在96分钟内即可从96个样本中纯化获得高品质核酸。QIAxtractor的优化试 剂可从不同的起始样本中纯化核酸,包括液体样本和组织。QIAxtractor极易使用,其配套的软件十分直观,您只需点击鼠标即可开始操作。

QIAxtractor具有新颖紧凑的设计,占地非常小。紧凑的设计使仪器易于移动,以适应样品制备要求。为了增加灵活性,与紧凑的设计相配套,QIAxtractor配有笔记本电脑。

先进的仪器安全性,包括半透明罩,有助于保护珍稀的样本不受环境污染。HEPA过滤器能维持在半透明罩下工作台上洁净的正向气压。紫外线可有效净化工作台,有助于防止交叉感染。

另有两款专有的QIAxtractor的产品线。QIAxtractor DX Reagents和Plasticware适用于纯化基因组DNA;QIAxtractor VX Reagents和Plasticware适用于纯化病毒核酸。试剂和耗材包含所有您制备样本所需的材料,包括纯化试剂和耗材,乙醇和一次性过滤头。这能 最大限度地给予用户方便,让您的实验快速进行。

产品
  • 产品目录
  • 自动化解决方案
    142
  • 样本破碎
    5
  • 样本制备
    33
  • 反应体系构建
    5
  • 检测与分析
    14
  • 焦磷酸测序
    19
  • 附件
    71
  • 服务与支持
    3
  • 分子诊断
  • 二代测序
  • 生物信息学
  • 生命科学领域研究
  • 人类身份认定与法医学检测
  • 动物和兽医学
  • 基因或通路特异性产品搜索
Quick order

Add to cart

订阅电子通讯

了解更多 订阅

QIAxtractor

打印 分享 加入收藏列表

Binding Reagent (VXB) (350 ml) 货号:950233

从各种类型样本中经济、高通量的纯化核酸

  • 经优化的操作流程,即使难处理样品也能获得高品质结果
  • 交叉污染的风险最小化,十分可靠
  • 方便、易于使用、占地面积小
  • 快速、自动的操作流程让你有更多时间用于其它任务
  • 运行前后完整的文档记录

QIAxtractor表现卓越,高度可靠,每次可从8–96个样品中纯化高品质核酸。创新的仪器特性确保可靠的纯化,将交叉污染的风险降至最低。QIAxtractor是完全整合的解决方案,提供试剂、耗材以及从各种样品中纯化核酸的优化操作流程。

  •  订购信息
  •  详细信息
  •  技术参数
  •  相关资料

性能
使用QIAxtractor纯化得到的核酸在灵敏的应用中有优越表现。通过QIAxtractor裂解样本,并且样本可以在仪器上,或脱离仪器进行孵育。特别设计的新型过滤头确保可靠的性能,有助于降低交叉污染的风险(参见High performance and reliability in sensitive applications)。

原理

QIAxtractor的自动纯化过程快速,并十分经济;在96分钟内即可从96个样本中纯化获得高品质核酸。QIAxtractor的优化试 剂可从不同的起始样本中纯化核酸,包括液体样本和组织。QIAxtractor极易使用,其配套的软件十分直观,您只需点击鼠标即可开始操作。

QIAxtractor具有新颖紧凑的设计,占地非常小。紧凑的设计使仪器易于移动,以适应样品制备要求。为了增加灵活性,与紧凑的设计相配套,QIAxtractor配有笔记本电脑。

先进的仪器安全性,包括半透明罩,有助于保护珍稀的样本不受环境污染。HEPA过滤器能维持在半透明罩下工作台上洁净的正向气压。紫外线可有效净化工作台,有助于防止交叉感染。

另有两款专有的QIAxtractor的产品线。QIAxtractor DX Reagents和Plasticware适用于纯化基因组DNA;QIAxtractor VX Reagents和Plasticware适用于纯化病毒核酸。试剂和耗材包含所有您制备样本所需的材料,包括纯化试剂和耗材,乙醇和一次性过滤头。这能 最大限度地给予用户方便,让您的实验快速进行。

操作流程

经优化的实验方案能处理液体样本和组织(参见下表)。只需选择最适合您起始样本的实验方案并启动运行。利用方便的软件向导,只需点击几下鼠标,即可开始操作。每次运行前和运行后的报告可提供完整的文档。

经优化的实验方案适用于各种样本类型

实验方案 裂解物 样本类型
液体 Buffer DXL 体液(血液、血浆、血清、尿液、呼吸道样本)和细胞悬浮液(白膜、细胞培养物)
组织 Buffer DXT 组织如活组织检查、鼠尾、对虾组织、鸟羽毛的羽髓、大脑、肝脏、肌肉等组织

QIAxtractor 可与您的工作流程无缝接合,与QIAGEN分析技术兼容。每次可自动纯化8至96个样品,解放您的双手,让您有更多时间处理其他事务。产物核酸可收集到 96个特别设计的管中,实现与您工作流程的接合。装有产物核酸离心管的洗脱板可转移到QIAgility上,自动构建PCR反应体系。可在Rotor- Gene Q实时荧光定量PCR分析仪上进行real-time PCR或RT-PCR,而终点式PCR可在QIAxcel上完成。

应用
纯化得到的核酸特别适用于敏感的应用,如:

  • 基因分型 
  • 生物标志物的测序研究
  • 基因组学研究
  • 生物医学研究