Super Fluor 647 SE, ,Super Fluor 647 SE,

Super Fluor 647 SE

有货

Super Fluor 647 SE, ,Super Fluor 647 SE,

品牌:Jinpan
Super Fluor 647 SE

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266349-1mg 1mg 期货 Super Fluor 647 SE, ,Super Fluor 647 SE,  

基本信息

产品名称 Super Fluor 647 SE
英文名称 Super Fluor 647 SE
运输条件 超低温冰袋运输

一般描述

Super Fluor 647 活化酯的性质参数

活化酯分子量:1300
最大吸收波长(nm):647
摩尔吸光系数(M-1cm-1):239000 

最大发射波长(nm):665
A280nm /Amax:3%

Super Fluor 标记抗体的参考方案

1) 蛋白溶解:用0.1 M NaHCO3溶解至终浓度为2mg/mL

1ml 去离子水加入84mgNaHCO3瓶中,配成1M 的碳酸氢钠溶液。振荡或吹打至完全溶解。该溶液pH 约8.3,2~6℃保存可用2 星期;若抗体是溶液,将抗体稀释至2mg/mL,再加入1/10 体积的上述碳酸氢钠溶液;若抗体是冻干粉,将1M 碳酸氢钠溶液用10 倍去离子水稀释成0.1M 后,用其将抗体溶成2mg/mL的溶液;

2)染料溶解:活化Super Flour SE染料固体粉末从冰箱放入干燥器中慢慢升至常温后,用无水DMSO将染料配置浓度为1 mg/mL。

3)标记:在1mL蛋白溶液中缓慢加入适量染料溶液,(蛋白:染料摩尔比=1:20~50;质量比可在以下范围内优化10~200ug Super Flour SE 每毫克IgG抗体)。同时在暗处常温缓慢搅拌1小时。也可以直接将蛋白溶液直接加入染料的固体粉末的试剂瓶中,同时在暗处常温缓慢搅拌1小时。

4)纯化:用透析法或者葡聚糖Sephadex纯化产品后,产品冷冻干燥成粉末或在0.01 M PBS/2mM叠氮化钠溶液中,-20℃避光储存。

5)贮存:标记好的蛋白贮存于2~6℃,避光。若纯化的蛋白偶联物浓度小于1mg/mL,加入BSA 或其他稳定蛋白1~10mg/ml。在2mM 叠氮钠存在的情况下,2~6 度可保存几个月。保存更长时间,则需分装后-20℃保存。避免反复冻融,避光保存。

计算标记比例F/P值

对于IgG 等大多数抗体来说,摩尔吸光系数为203000,F/P值在4~9 之间是最合适。

Super Flour 647标记蛋白F/P值计算:

1)用PBS 精确倍数稀释定量的少许纯化过的偶联蛋白,在1cm比色皿中测定280nm和650nm的光吸收;

2)计算样品中的蛋白浓度:蛋白摩尔浓度=(A280-(A650×0.03))×稀释倍数/203000

3)计算标记比例:每摩尔蛋白结合的染料摩尔数=A650×稀释倍数/(239000×蛋白摩尔浓度)

活性染料稳定性与储存

请注意:未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 647 activated ester

Activated ester molecular weight: 1300
Maximum absorption wavelength (nm): 647
Molar absorption coefficient (M-1cm-1): 239000

Maximum emission wavelength (nm): 665
A280nm /Amax: 3%

Reference scheme for Super Fluor labeled antibodies

1) Protein dissolution: Dissolve with 0.1 M NaHCO3 to a final concentration of 2mg/mL

Add 1ml of deionized water to an 84mg NaHCO3 bottle to make a 1M sodium bicarbonate solution. Shake or pipette until completely dissolved. The pH of the solution is about 8.3, and it can be stored at 2-6°C for 2 weeks; if the antibody is a solution, dilute the antibody to 2mg/mL, then add 1/10 volume of the above sodium bicarbonate solution; if the antibody is a lyophilized powder, add 1M After the sodium bicarbonate solution is diluted with 10 times deionized water to 0.1M, use it to dissolve the antibody into a 2mg/mL solution;

2) Dye dissolution: After the activated Super Flour SE dye solid powder is slowly warmed to room temperature in a desiccator from the refrigerator, the dye preparation concentration is 1 mg/mL with anhydrous DMSO.

3) Labeling: Slowly add an appropriate amount of dye solution to 1 mL of protein solution, (protein: dye molar ratio = 1:20-50; the mass ratio can be optimized within the following range of 10-200ug Super Flour SE per mg of IgG antibody). At the same time, slowly stir for 1 hour at room temperature in the dark. It is also possible to directly add the protein solution directly to the reagent bottle of the solid powder of the dye, and at the same time, slowly stir for 1 hour at room temperature in the dark.

4) Purification: After purifying the product by dialysis or Sephadex, the product is freeze-dried into powder or stored in 0.01 M PBS/2mM sodium azide solution at -20°C and protected from light.

5) Storage: Store the labeled protein at 2~6℃, away from light. If the concentration of the purified protein conjugate is less than 1 mg/mL, add BSA or other stable proteins at 1-10 mg/ml. In the presence of 2mM sodium azide, it can be stored for several months at 2-6 degrees. For longer storage, you need to store at -20°C after packaging. Avoid repeated freezing and thawing, and store in the dark.

Calculate the mark ratio F/P value

For most antibodies such as IgG, the molar absorption coefficient is 203000, and the F/P value between 4-9 is the most appropriate.

Super Flour 647 labeled protein F/P value calculation:

1) Dilute quantitatively a few purified coupled proteins with PBS with precise multiples, and measure the light absorption at 280nm and 650nm in a 1cm cuvette;

2) Calculate the protein concentration in the sample: protein molar concentration=(A280-(A650×0.03))×dilution multiple/203000

3) Calculate the labeling ratio: moles of dye bound per mole of protein = A650 × dilution factor / (239000 × protein molar concentration)

Reactive dye stability and storage

Please note: the unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 750, ,Super Fluor 750,

Super Fluor 750

有货

Super Fluor 750, ,Super Fluor 750,

品牌:Jinpan
Super Fluor 750

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266401-5mg 5mg 期货 Super Fluor 750, ,Super Fluor 750,  

基本信息

产品名称 Super Fluor 750
英文名称 Super Fluor 750
运输条件 超低温冰袋运输

一般描述

Super Fluor 750 活化酯的性质参数

活化酯分子量: 1300
最大吸收波长(nm): 750
摩尔吸光系数(M-1cm-1): 240000 

最大发射波长(nm): 775
A280nm /Amax: 4%

原料准备

A.活性染料 1mg;B.碳酸氢钠 ~84mg;C.抗体纯化树脂P-30/Sephadex G25M/透析袋;D.空柱 5 个;E.收集管 5 个;

注意:纯化的蛋白buffer 中不得含有铵离子或伯铵,因为它们会与蛋白质竞争结合活性染料。不纯的抗体或者含BSA 或明胶的抗体标记效果不好。低浓度的NaN3(<3mM)或thimerosal(<1mM)不影响偶联效果。

活性染料稳定性与储存

未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 750 activated ester

Activated ester molecular weight: 1300
Maximum absorption wavelength (nm): 750
Molar absorption coefficient (M-1cm-1): 240000

Maximum emission wavelength (nm): 775
A280nm /Amax: 4%

Raw material preparation

A. Reactive dye 1mg; B. Sodium bicarbonate ~ 84mg; C. Antibody purification resin P-30/Sephadex G25M/dialysis bag; D. 5 empty columns; E. 5 collection tubes;

Note: The purified protein buffer must not contain ammonium ions or primary ammonium ions, because they will compete with the protein to bind reactive dyes. Impure antibodies or antibodies containing BSA or gelatin are not well labeled. Low concentration of NaN3 (<3mM) or thimerosal (<1mM) does not affect the coupling effect.

Reactive dye stability and storage

The unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 750 SE, ,Super Fluor 750 SE,

Super Fluor 750 SE

有货

Super Fluor 750 SE, ,Super Fluor 750 SE,

品牌:Jinpan
Super Fluor 750 SE

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266356-1mg 1mg 期货 Super Fluor 750 SE, ,Super Fluor 750 SE,  

基本信息

产品名称 Super Fluor 750 SE
英文名称 Super Fluor 750 SE
运输条件 超低温冰袋运输

一般描述

Super Fluor 750 活化酯的性质参数

活化酯分子量:1300
最大吸收波长(nm):750
摩尔吸光系数(M-1cm-1):240000 

最大发射波长(nm):775
A280nm /Amax:4%

Super Fluor 标记抗体的参考方案

1) 蛋白溶解:用0.1 M NaHCO3溶解至终浓度为2mg/mL

1ml 去离子水加入84mgNaHCO3瓶中,配成1M 的碳酸氢钠溶液。振荡或吹打至完全溶解。该溶液pH 约8.3,2~6℃保存可用2 星期;若抗体是溶液,将抗体稀释至2mg/mL,再加入1/10 体积的上述碳酸氢钠溶液;若抗体是冻干粉,将1M 碳酸氢钠溶液用10 倍去离子水稀释成0.1M 后,用其将抗体溶成2mg/mL的溶液

2)染料溶解:活化Super Flour SE染料固体粉末从冰箱放入干燥器中慢慢升至常温后,用无水DMSO将染料配置浓度为1 mg/mL。

3)标记:在1mL蛋白溶液中缓慢加入适量染料溶液,(蛋白:染料摩尔比=1:20~50;质量比可在以下范围内优化10~200ug Super Flour SE 每毫克IgG抗体)。同时在暗处常温缓慢搅拌1小时。也可以直接将蛋白溶液直接加入染料的固体粉末的试剂瓶中,同时在暗处常温缓慢搅拌1小时。

4)纯化:用透析法或者葡聚糖Sephadex纯化产品后,产品冷冻干燥成粉末或在0.01 M PBS/2mM叠氮化钠溶液中,-20℃避光储存。

5)贮存:标记好的蛋白贮存于2~6℃,避光。若纯化的蛋白偶联物浓度小于1mg/mL,加入BSA 或其他稳定蛋白1~10mg/ml。在2mM 叠氮钠存在的情况下,2~6 度可保存几个月。保存更长时间,则需分装后-20℃保存。避免反复冻融,避光保存。

计算标记比例F/P值

对于IgG 等大多数抗体来说,摩尔吸光系数为203000,F/P值在4~9 之间是最合适。

Super Flour 750标记蛋白F/P值计算:

1)用PBS 精确倍数稀释定量的少许纯化过的偶联蛋白,在1cm比色皿中测定280nm和750nm的光吸收

2)计算样品中的蛋白浓度:蛋白摩尔浓度=(A280-(A750×0.04))×稀释倍数/203000

3)计算标记比例:每摩尔蛋白结合的染料摩尔数=A750×稀释倍数/(240000×蛋白摩尔浓度)

活性染料稳定性与储存

请注意:未开封的粉末在避光干燥-20℃可存放6个月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 750 activated ester

Activated ester molecular weight: 1300
Maximum absorption wavelength (nm): 750
Molar absorption coefficient (M-1cm-1): 240000

Maximum emission wavelength (nm): 775
A280nm /Amax: 4%

Reference scheme for Super Fluor labeled antibodies

1) Protein dissolution: Dissolve with 0.1 M NaHCO3 to a final concentration of 2mg/mL

Add 1ml of deionized water to an 84mg NaHCO3 bottle to make a 1M sodium bicarbonate solution. Shake or pipette until completely dissolved. The pH of the solution is about 8.3, and it can be stored at 2-6°C for 2 weeks; if the antibody is a solution, dilute the antibody to 2mg/mL, then add 1/10 volume of the above sodium bicarbonate solution; if the antibody is a lyophilized powder, add 1M After the sodium bicarbonate solution is diluted with 10 times deionized water to 0.1M, use it to dissolve the antibody into a 2mg/mL solution.

2) Dye dissolution: After the activated Super Flour SE dye solid powder is slowly warmed to room temperature in a desiccator from the refrigerator, the dye preparation concentration is 1 mg/mL with anhydrous DMSO.

3) Labeling: Slowly add an appropriate amount of dye solution to 1 mL of protein solution, (protein: dye molar ratio = 1:20-50; the mass ratio can be optimized within the following range of 10-200ug Super Flour SE per mg of IgG antibody). At the same time, slowly stir for 1 hour at room temperature in the dark. It is also possible to directly add the protein solution directly to the reagent bottle of the solid powder of the dye, and at the same time, slowly stir for 1 hour at room temperature in the dark.

4) Purification: After purifying the product by dialysis or Sephadex, the product is freeze-dried into powder or stored in 0.01 M PBS/2mM sodium azide solution at -20°C and protected from light.

5) Storage: Store the labeled protein at 2~6℃, away from light. If the concentration of the purified protein conjugate is less than 1 mg/mL, add BSA or other stable proteins at 1-10 mg/ml. In the presence of 2mM sodium azide, it can be stored for several months at 2-6 degrees. For longer storage, you need to store at -20°C after packaging. Avoid repeated freezing and thawing, and store in the dark.

Calculate the mark ratio F/P value

For most antibodies such as IgG, the molar absorption coefficient is 203000, and the F/P value between 4-9 is the most appropriate.

Super Flour 750 labeled protein F/P value calculation:

1) Dilute quantitatively a few purified coupled proteins with PBS with precise multiples, and measure the light absorption at 280nm and 750nm in a 1cm cuvette

2) Calculate the protein concentration in the sample: protein molar concentration=(A280-(A750×0.04))×dilution multiple/203000

3) Calculate the labeling ratio: moles of dye bound per mole of protein = A750 × dilution factor/(240000 × protein molar concentration)

Reactive dye stability and storage

Please note: the unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 647, ,Super Fluor 647,

Super Fluor 647

有货

Super Fluor 647, ,Super Fluor 647,

品牌:Jinpan
Super Fluor 647

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266384-5mg 5mg 期货 Super Fluor 647, ,Super Fluor 647,  

基本信息

产品名称 Super Fluor 647
英文名称 Super Fluor 647
运输条件 超低温冰袋运输

一般描述

Super Fluor 647 活化酯的性质参数

活化酯分子量:1300
最大吸收波长(nm):647
摩尔吸光系数(M-1cm-1):239000 

最大发射波长(nm):665
A280nm /Amax:3%

Super Fluor 标记抗体的参考方案

1) 蛋白溶解:用0.1 M NaHCO3溶解至终浓度为2mg/mL

1ml 去离子水加入84mgNaHCO3瓶中,配成1M 的碳酸氢钠溶液。振荡或吹打至完全溶解。该溶液pH 约8.3,2~6℃保存可用2 星期;若抗体是溶液,将抗体稀释至2mg/mL,再加入1/10 体积的上述碳酸氢钠溶液;若抗体是冻干粉,将1M 碳酸氢钠溶液用10 倍去离子水稀释成0.1M 后,用其将抗体溶成2mg/mL的溶液;

2)染料溶解:活化Super Flour SE染料固体粉末从冰箱放入干燥器中慢慢升至常温后,用无水DMSO将染料配置浓度为1 mg/mL。

3)标记:在1mL蛋白溶液中缓慢加入适量染料溶液,(蛋白:染料摩尔比=1:20~50;质量比可在以下范围内优化10~200ug Super Flour SE 每毫克IgG抗体)。同时在暗处常温缓慢搅拌1小时。也可以直接将蛋白溶液直接加入染料的固体粉末的试剂瓶中,同时在暗处常温缓慢搅拌1小时。

4)纯化:用透析法或者葡聚糖Sephadex纯化产品后,产品冷冻干燥成粉末或在0.01 M PBS/2mM叠氮化钠溶液中,-20℃避光储存。

5)贮存:标记好的蛋白贮存于2~6℃,避光。若纯化的蛋白偶联物浓度小于1mg/mL,加入BSA 或其他稳定蛋白1~10mg/ml。在2mM 叠氮钠存在的情况下,2~6 度可保存几个月。保存更长时间,则需分装后-20℃保存。避免反复冻融,避光保存。

计算标记比例F/P值

对于IgG 等大多数抗体来说,摩尔吸光系数为203000,F/P值在4~9 之间是最合适。

Super Flour 647标记蛋白F/P值计算:

1)用PBS 精确倍数稀释定量的少许纯化过的偶联蛋白,在1cm比色皿中测定280nm和650nm的光吸收;

2)计算样品中的蛋白浓度:蛋白摩尔浓度=(A280-(A650×0.03))×稀释倍数/203000

3)计算标记比例:每摩尔蛋白结合的染料摩尔数=A650×稀释倍数/(239000×蛋白摩尔浓度)

活性染料稳定性与储存

请注意:未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 647 activated ester

Activated ester molecular weight: 1300
Maximum absorption wavelength (nm): 647
Molar absorption coefficient (M-1cm-1): 239000

Maximum emission wavelength (nm): 665
A280nm /Amax: 3%

Reference scheme for Super Fluor labeled antibodies

1) Protein dissolution: Dissolve with 0.1 M NaHCO3 to a final concentration of 2mg/mL

Add 1ml of deionized water to an 84mg NaHCO3 bottle to make a 1M sodium bicarbonate solution. Shake or pipette until completely dissolved. The pH of the solution is about 8.3, and it can be stored at 2-6°C for 2 weeks; if the antibody is a solution, dilute the antibody to 2mg/mL, then add 1/10 volume of the above sodium bicarbonate solution; if the antibody is a lyophilized powder, add 1M After the sodium bicarbonate solution is diluted with 10 times deionized water to 0.1M, use it to dissolve the antibody into a 2mg/mL solution;

2) Dye dissolution: After the activated Super Flour SE dye solid powder is slowly warmed to room temperature in a desiccator from the refrigerator, the dye preparation concentration is 1 mg/mL with anhydrous DMSO.

3) Labeling: Slowly add an appropriate amount of dye solution to 1 mL of protein solution, (protein: dye molar ratio = 1:20-50; the mass ratio can be optimized within the following range of 10-200ug Super Flour SE per mg of IgG antibody). At the same time, slowly stir for 1 hour at room temperature in the dark. It is also possible to directly add the protein solution directly to the reagent bottle of the solid powder of the dye, and at the same time, slowly stir for 1 hour at room temperature in the dark.

4) Purification: After purifying the product by dialysis or Sephadex, the product is freeze-dried into powder or stored in 0.01 M PBS/2mM sodium azide solution at -20°C and protected from light.

5) Storage: Store the labeled protein at 2~6℃, away from light. If the concentration of the purified protein conjugate is less than 1 mg/mL, add BSA or other stable proteins at 1-10 mg/ml. In the presence of 2mM sodium azide, it can be stored for several months at 2-6 degrees. For longer storage, you need to store at -20°C after packaging. Avoid repeated freezing and thawing, and store in the dark.

Calculate the mark ratio F/P value

For most antibodies such as IgG, the molar absorption coefficient is 203000, and the F/P value between 4-9 is the most appropriate.

Super Flour 647 labeled protein F/P value calculation:

1) Dilute quantitatively a few purified coupled proteins with PBS with precise multiples, and measure the light absorption at 280nm and 650nm in a 1cm cuvette;

2) Calculate the protein concentration in the sample: protein molar concentration=(A280-(A650×0.03))×dilution multiple/203000

3) Calculate the labeling ratio: moles of dye bound per mole of protein = A650 × dilution factor / (239000 × protein molar concentration)

Reactive dye stability and storage

Please note: the unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 488, ,Super Fluor 488,

Super Fluor 488

有货

Super Fluor 488, ,Super Fluor 488,

品牌:Jinpan
Super Fluor 488

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266374-5mg 5mg 期货 Super Fluor 488, ,Super Fluor 488,  

基本信息

产品名称 Super Fluor 488
英文名称 Super Fluor 488
运输条件 超低温冰袋运输

一般描述

Super Fluor 488活化酯的性质参数

活化酯分子量:900

最大吸收波长(nm):494

摩尔吸光系数(M-1cm-1):71000

最大发射波长(nm):524

A280nm /Amax:11%

原料准备

A.活性染料 1mg;B.碳酸氢钠 ~84mg;C.抗体纯化树脂P-30/Sephadex G25M/透析袋;D.空柱 5 个;E.收集管 5 个;

注意:纯化的蛋白buffer 中不得含有铵离子或伯铵,因为它们会与蛋白质竞争结合活性染料。不纯的抗体或者含BSA 或明胶的抗体标记效果不好。低浓度的NaN3(<3mM)或thimerosal(<1mM)不影响偶联效果。

活性染料稳定性与储存

未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 488 activated ester

Activated ester molecular weight: 900

Maximum absorption wavelength (nm): 494

Molar absorption coefficient (M-1cm-1): 71000

Maximum emission wavelength (nm): 524

A280nm /Amax: 11%

Raw material preparation

A. Reactive dye 1mg; B. Sodium bicarbonate ~ 84mg; C. Antibody purification resin P-30/Sephadex G25M/dialysis bag; D. 5 empty columns; E. 5 collection tubes;

Note: The purified protein buffer must not contain ammonium ions or primary ammonium ions, because they will compete with the protein to bind reactive dyes. Impure antibodies or antibodies containing BSA or gelatin are not well labeled. Low concentration of NaN3 (<3mM) or thimerosal (<1mM) does not affect the coupling effect.

Reactive dye stability and storage

The unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 555, ,Super Fluor 555,

Super Fluor 555

有货

Super Fluor 555, ,Super Fluor 555,

品牌:Jinpan
Super Fluor 555

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266378-5mg 5mg 期货 Super Fluor 555, ,Super Fluor 555,  

基本信息

产品名称 Super Fluor 555
英文名称 Super Fluor 555
运输条件 超低温冰袋运输

一般描述

Super Fluor 555 活化酯的性质参数

活化酯分子量:1250
最大吸收波长(nm):555
摩尔吸光系数(M-1cm-1):150000 

最大发射波长(nm):565
A280nm /Amax:8%

原料准备

A.活性染料 1mg;B.碳酸氢钠 ~84mg;C.抗体纯化树脂P-30/Sephadex G25M/透析袋;D.空柱 5 个;E.收集管 5 个;

注意:纯化的蛋白buffer 中不得含有铵离子或伯铵,因为它们会与蛋白质竞争结合活性染料。不纯的抗体或者含BSA 或明胶的抗体标记效果不好。低浓度的NaN3(<3mM)或thimerosal(<1mM)不影响偶联效果。

活性染料稳定性与储存

未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 555 activated ester

Activated ester molecular weight: 1250
Maximum absorption wavelength (nm): 555
Molar absorption coefficient (M-1cm-1): 150000

Maximum emission wavelength (nm): 565
A280nm / Amax: 8%

Raw material preparation

A. Reactive dye 1mg; B. Sodium bicarbonate ~ 84mg; C. Antibody purification resin P-30/Sephadex G25M/dialysis bag; D. 5 empty columns; E. 5 collection tubes;

Note: The purified protein buffer must not contain ammonium ions or primary ammonium ions, because they will compete with the protein to bind reactive dyes. Impure antibodies or antibodies containing BSA or gelatin are not well labeled. Low concentration of NaN3 (<3mM) or thimerosal (<1mM) does not affect the coupling effect.

Reactive dye stability and storage

The unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 555 SE, ,Super Fluor 555 SE,

Super Fluor 555 SE

有货

Super Fluor 555 SE, ,Super Fluor 555 SE,

品牌:Jinpan
Super Fluor 555 SE

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266344-1mg 1mg 期货 Super Fluor 555 SE, ,Super Fluor 555 SE,  

基本信息

产品名称 Super Fluor 555 SE
英文名称 Super Fluor 555 SE
运输条件 超低温冰袋运输

一般描述

Super Fluor 555 活化酯的性质参数

活化酯分子量:1250
最大吸收波长(nm):555
摩尔吸光系数(M-1cm-1):150000 

最大发射波长(nm):565
A280nm /Amax:8%

Super Fluor 标记抗体的参考方案

1) 蛋白溶解:用0.1 M NaHCO3溶解至终浓度为2mg/mL

1ml 去离子水加入84mgNaHCO3瓶中,配成1M 的碳酸氢钠溶液。振荡或吹打至完全溶解。该溶液pH 约8.3,2~6℃保存可用2 星期;若抗体是溶液,将抗体稀释至2mg/mL,再加入1/10 体积的上述碳酸氢钠溶液;若抗体是冻干粉,将1M 碳酸氢钠溶液用10 倍去离子水稀释成0.1M 后,用其将抗体溶成2mg/mL的溶液;

2)染料溶解:活化Super Flour SE染料固体粉末从冰箱放入干燥器中慢慢升至常温后,用无水DMSO将染料配置浓度为1 mg/mL。

3)标记:在1mL蛋白溶液中缓慢加入适量染料溶液,(蛋白:染料摩尔比=1:20~50;质量比可在以下范围内优化10~200ug Super Flour SE 每毫克IgG抗体)。同时在暗处常温缓慢搅拌1小时。也可以直接将蛋白溶液直接加入染料的固体粉末的试剂瓶中,同时在暗处常温缓慢搅拌1小时。

4)纯化:用透析法或者葡聚糖Sephadex纯化产品后,产品冷冻干燥成粉末或在0.01 M PBS/2mM叠氮化钠溶液中,-20℃避光储存。

5)贮存:标记好的蛋白贮存于2~6℃,避光。若纯化的蛋白偶联物浓度小于1mg/mL,加入BSA 或其他稳定蛋白1~10mg/ml。在2mM 叠氮钠存在的情况下,2~6 度可保存几个月。保存更长时间,则需分装后-20℃保存。避免反复冻融,避光保存。

计算标记比例F/P值

对于IgG 等大多数抗体来说,摩尔吸光系数为203000,F/P值在4~9 之间是最合适。

Super Flour 555标记蛋白F/P值计算:

1)用PBS 精确倍数稀释定量的少许纯化过的偶联蛋白,在1cm比色皿中测定280nm和555nm的光吸收;

2)计算样品中的蛋白浓度:蛋白摩尔浓度=(A280-(A555×0.08))×稀释倍数/203000

3)计算标记比例:每摩尔蛋白结合的染料摩尔数=A555×稀释倍数/(150000×蛋白摩尔浓度)

活性染料稳定性与储存

请注意:未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 555 activated ester

Activated ester molecular weight: 1250
Maximum absorption wavelength (nm): 555
Molar absorption coefficient (M-1cm-1): 150000

Maximum emission wavelength (nm): 565
A280nm / Amax: 8%

Reference scheme for Super Fluor labeled antibodies

1) Protein dissolution: Dissolve with 0.1 M NaHCO3 to a final concentration of 2mg/mL

Add 1ml of deionized water to an 84mg NaHCO3 bottle to make a 1M sodium bicarbonate solution. Shake or pipette until completely dissolved. The pH of the solution is about 8.3, and it can be stored at 2-6°C for 2 weeks; if the antibody is a solution, dilute the antibody to 2mg/mL, then add 1/10 volume of the above sodium bicarbonate solution; if the antibody is a lyophilized powder, add 1M After the sodium bicarbonate solution is diluted with 10 times deionized water to 0.1M, use it to dissolve the antibody into a 2mg/mL solution;

2) Dye dissolution: After the activated Super Flour SE dye solid powder is slowly warmed to room temperature in a desiccator from the refrigerator, the dye preparation concentration is 1 mg/mL with anhydrous DMSO.

3) Labeling: Slowly add an appropriate amount of dye solution to 1 mL of protein solution, (protein: dye molar ratio = 1:20-50; the mass ratio can be optimized within the following range of 10-200ug Super Flour SE per mg of IgG antibody). At the same time, slowly stir for 1 hour at room temperature in the dark. It is also possible to directly add the protein solution directly to the reagent bottle of the solid powder of the dye, and at the same time, slowly stir for 1 hour at room temperature in the dark.

4) Purification: After purifying the product by dialysis or Sephadex, the product is freeze-dried into powder or stored in 0.01 M PBS/2mM sodium azide solution at -20°C and protected from light.

5) Storage: Store the labeled protein at 2~6℃, away from light. If the concentration of the purified protein conjugate is less than 1 mg/mL, add BSA or other stable proteins at 1-10 mg/ml. In the presence of 2mM sodium azide, it can be stored for several months at 2-6 degrees. For longer storage, you need to store at -20°C after packaging. Avoid repeated freezing and thawing, and store in the dark.

Calculate the mark ratio F/P value

For most antibodies such as IgG, the molar absorption coefficient is 203000, and the F/P value between 4-9 is the most appropriate.

Super Flour 555 labeled protein F/P value calculation

1) Dilute quantitatively a few purified coupled proteins with PBS with precise multiples, and measure the light absorption at 280nm and 555nm in a 1cm cuvette;

2) Calculate the protein concentration in the sample: protein molar concentration=(A280-(A555×0.08))×dilution multiple/203000

3) Calculate the labeling ratio: moles of dye bound per mole of protein = A555 × dilution factor / (150000 × protein molar concentration)

Reactive dye stability and storage

Please note: the unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 680, ,Super Fluor 680,

Super Fluor 680

有货

Super Fluor 680, ,Super Fluor 680,

品牌:Jinpan
Super Fluor 680

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266399-5mg 5mg 期货 Super Fluor 680, ,Super Fluor 680,  

基本信息

产品名称 Super Fluor 680
英文名称 Super Fluor 680
运输条件 超低温冰袋运输

一般描述

Super Fluor 680活化酯的性质参数

活化酯分子量:1150
最大吸收波长(nm):680
摩尔吸光系数(M-1cm-1):184000 

最大发射波长(nm):702
A280nm /Amax:5%

原料准备

A.活性染料 1mg;B.碳酸氢钠 ~84mg;C.抗体纯化树脂P-30/Sephadex G25M/透析袋;D.空柱 5 个;E.收集管 5 个;

注意:纯化的蛋白buffer 中不得含有铵离子或伯铵,因为它们会与蛋白质竞争结合活性染料。不纯的抗体或者含BSA 或明胶的抗体标记效果不好。低浓度的NaN3(<3mM)或thimerosal(<1mM)不影响偶联效果。

活性染料稳定性与储存

未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 680 activated ester

Activated ester molecular weight: 1150
Maximum absorption wavelength (nm): 680
Molar absorption coefficient (M-1cm-1): 184000

Maximum emission wavelength (nm): 702
A280nm / Amax: 5%

Raw material preparation

A. Reactive dye 1mg; B. Sodium bicarbonate ~ 84mg; C. Antibody purification resin P-30/Sephadex G25M/dialysis bag; D. 5 empty columns; E. 5 collection tubes;

Note: The purified protein buffer must not contain ammonium ions or primary ammonium ions, because they will compete with the protein to bind reactive dyes. Impure antibodies or antibodies containing BSA or gelatin are not well labeled. Low concentration of NaN3 (<3mM) or thimerosal (<1mM) does not affect the coupling effect.

Reactive dye stability and storage

The unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

 

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 680 SE, ,Super Fluor 680 SE,

Super Fluor 680 SE

有货

Super Fluor 680 SE, ,Super Fluor 680 SE,

品牌:Jinpan
Super Fluor 680 SE

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266352-1mg 1mg 期货 Super Fluor 680 SE, ,Super Fluor 680 SE,  

基本信息

产品名称 Super Fluor 680 SE
英文名称 Super Fluor 680 SE
运输条件 超低温冰袋运输

一般描述

Super Fluor 680活化酯的性质参数

活化酯分子量:1150
最大吸收波长(nm):680
摩尔吸光系数(M-1cm-1):184000 

最大发射波长(nm):702
A280nm /Amax:5%

Super Fluor 标记抗体的参考方案

1) 蛋白溶解:用0.1 M NaHCO3溶解至终浓度为2mg/mL

1ml 去离子水加入84mgNaHCO3瓶中,配成1M 的碳酸氢钠溶液。振荡或吹打至完全溶解。该溶液pH 约8.3,2~6℃保存可用2 星期;若抗体是溶液,将抗体稀释至2mg/mL,再加入1/10 体积的上述碳酸氢钠溶液;若抗体是冻干粉,将1M 碳酸氢钠溶液用10 倍去离子水稀释成0.1M 后,用其将抗体溶成2mg/mL的溶液;

2)染料溶解:活化Super Flour SE染料固体粉末从冰箱放入干燥器中慢慢升至常温后,用无水DMSO将染料配置浓度为1 mg/mL。

3)标记:在1mL蛋白溶液中缓慢加入适量染料溶液,(蛋白:染料摩尔比=1:20~50;质量比可在以下范围内优化10~200ug Super Flour SE 每毫克IgG抗体)。同时在暗处常温缓慢搅拌1小时。也可以直接将蛋白溶液直接加入染料的固体粉末的试剂瓶中,同时在暗处常温缓慢搅拌1小时。

4)纯化:用透析法或者葡聚糖Sephadex纯化产品后,产品冷冻干燥成粉末或在0.01 M PBS/2mM叠氮化钠溶液中,-20℃避光储存。

5)贮存:标记好的蛋白贮存于2~6℃,避光。若纯化的蛋白偶联物浓度小于1mg/mL,加入BSA 或其他稳定蛋白1~10mg/ml。在2mM 叠氮钠存在的情况下,2~6 度可保存几个月。保存更长时间,则需分装后-20℃保存。避免反复冻融,避光保存。

计算标记比例F/P值

对于IgG 等大多数抗体来说,摩尔吸光系数为203000,F/P值在4~9 之间是最合适。

Super Flour 680标记蛋白F/P值计算:

1)用PBS 精确倍数稀释定量的少许纯化过的偶联蛋白,在1cm比色皿中测定280nm和680nm的光吸收

2)计算样品中的蛋白浓度:蛋白摩尔浓度=(A280-(A650×0.05))×稀释倍数/203000

3)计算标记比例:每摩尔蛋白结合的染料摩尔数=A650×稀释倍数/(184000×蛋白摩尔浓度)

活性染料稳定性与储存

请注意:未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

Properties of Super Fluor 680 activated ester

Activated ester molecular weight: 1150
Maximum absorption wavelength (nm): 680
Molar absorption coefficient (M-1cm-1): 184000

Maximum emission wavelength (nm): 702
A280nm / Amax: 5%

Reference scheme for Super Fluor labeled antibodies

1) Protein dissolution: Dissolve with 0.1 M NaHCO3 to a final concentration of 2mg/mL

Add 1ml of deionized water to an 84mg NaHCO3 bottle to make a 1M sodium bicarbonate solution. Shake or pipette until completely dissolved. The pH of the solution is about 8.3, and it can be stored at 2-6°C for 2 weeks; if the antibody is a solution, dilute the antibody to 2mg/mL, then add 1/10 volume of the above sodium bicarbonate solution; if the antibody is a lyophilized powder, add 1M After the sodium bicarbonate solution is diluted with 10 times deionized water to 0.1M, use it to dissolve the antibody into a 2mg/mL solution;

2) Dye dissolution: After the activated Super Flour SE dye solid powder is slowly warmed to room temperature in a desiccator from the refrigerator, the dye preparation concentration is 1 mg/mL with anhydrous DMSO.

3) Labeling: Slowly add an appropriate amount of dye solution to 1 mL of protein solution, (protein: dye molar ratio = 1:20-50; the mass ratio can be optimized within the following range of 10-200ug Super Flour SE per mg of IgG antibody). At the same time, slowly stir for 1 hour at room temperature in the dark. It is also possible to directly add the protein solution directly to the reagent bottle of the solid powder of the dye, and at the same time, slowly stir for 1 hour at room temperature in the dark.

4) Purification: After purifying the product by dialysis or Sephadex, the product is freeze-dried into powder or stored in 0.01 M PBS/2mM sodium azide solution at -20°C and protected from light.

5) Storage: Store the labeled protein at 2~6℃, away from light. If the concentration of the purified protein conjugate is less than 1 mg/mL, add BSA or other stable proteins at 1-10 mg/ml. In the presence of 2mM sodium azide, it can be stored for several months at 2-6 degrees. For longer storage, you need to store at -20°C after packaging. Avoid repeated freezing and thawing, and store in the dark.

Calculate the mark ratio F/P value

For most antibodies such as IgG, the molar absorption coefficient is 203000, and the F/P value between 4-9 is the most appropriate.

Super Flour 680 labeled protein F/P value calculation:

1) Dilute quantitatively a few purified coupled proteins with PBS with precise multiples, and measure the light absorption at 280nm and 680nm in a 1cm cuvette

2) Calculate the protein concentration in the sample: protein molar concentration=(A280-(A650×0.05))×dilution multiple/203000

3) Calculate the labeling ratio: moles of dye bound per mole of protein = A650 × dilution factor / (184000 × protein molar concentration)

Reactive dye stability and storage

Please note: the unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan

Super Fluor 488 NHS, ,Super Fluor 488 NHS,

Super Fluor 488 NHS

有货

Super Fluor 488 NHS, ,Super Fluor 488 NHS,

品牌:Jinpan
Super Fluor 488 NHS

MSDS

质检证书(CoA)

相似产品

货号 (SKU) 包装规格 是否现货 价格 数量
S266301-1mg 1mg 期货 Super Fluor 488 NHS, ,Super Fluor 488 NHS,  

基本信息

产品名称 Super Fluor 488 NHS
英文名称 Super Fluor 488 NHS
运输条件 超低温冰袋运输

一般描述

1.产品简介  

Super Fluor 750 SE=Alexa Fluor 750 SE; Super Flour 系列(效果同Alexa Fluor 系列)荧光探针,具有更强的荧光强度,更广的pH应用范围(pH 4~10), 更好的抗淬灭性。在生物荧光领域已逐渐替代FITC,Cy3, Cy5, Cy5.5, Cy7等荧光染料。 

 Super Fluor 488,555, 647, 680, 750活化酯的性质参数

 

488

555

647

680

750

活化酯分子量

~900

 ~1250

 ~1300

~1150

 ~1300

最大吸收波长(nm)

494

555

647

680

750

摩尔吸光系数(M-1cm-1)

71000

150000

239000

184000

240000

最大发射波长(nm)

524

565

665

702

775

A280nm/Amax

11%

8%

3%

5%

4%

2. Super Fluor 标记抗体的参考方案

1) 蛋白溶解:用0.1 M NaHCO3溶解至终浓度为2mg/mL

1ml 去离子水加入84mgNaHCO3瓶中,配成1M 的碳酸氢钠溶液。振荡或吹打至完全溶解。该溶液pH 约8.3,2~6℃保存可用2 星期;若抗体是溶液,将抗体稀释至2mg/mL,再加入1/10 体积的上述碳酸氢钠溶液;若抗体是冻干粉,将1M 碳酸氢钠溶液用10 倍去离子水稀释成0.1M 后,用其将抗体溶成2mg/mL的溶液

2)染料溶解:活化Super Flour SE染料固体粉末从冰箱放入干燥器中慢慢升至常温后,用无水DMSO将染料配置浓度为1 mg/mL。

3)标记:在1mL蛋白溶液中缓慢加入适量染料溶液,(蛋白:染料摩尔比=1:20~50;质量比可在以下范围内优化10~200ug Super Flour SE 每毫克IgG抗体)。同时在暗处常温缓慢搅拌1小时。也可以直接将蛋白溶液直接加入染料的固体粉末的试剂瓶中,同时在暗处常温缓慢搅拌1小时。

4)纯化:用透析法或者葡聚糖Sephadex纯化产品后,产品冷冻干燥成粉末或在0.01 M PBS/2mM叠氮化钠溶液中,-20℃避光储存。

5)贮存:标记好的蛋白贮存于2~6℃,避光。若纯化的蛋白偶联物浓度小于1mg/mL,加入BSA 或其他稳定蛋白1~10mg/ml。在2mM 叠氮钠存在的情况下,2~6 度可保存几个月。保存更长时间,则需分装后-20℃保存。避免反复冻融,避光保存。

3.计算标记比例F/P值

对于IgG 等大多数抗体来说,摩尔吸光系数为203000,F/P值在4~9 之间是最合适。

Super Flour 488标记蛋白F/P值计算:

1)用PBS 精确倍数稀释定量的少许纯化过的偶联蛋白,在1cm比色皿中测定280nm和494nm的光吸收;

2)计算样品中的蛋白浓度:蛋白摩尔浓度=(A280-(A494×0.11))×稀释倍数/203000

3)计算标记比例:每摩尔蛋白结合的染料摩尔数=A494×稀释倍数/(71000×蛋白摩尔浓度)

4. 活性染料稳定性与储存

 请注意:未开封的粉末在避光干燥-20℃可存放6月。其水溶液现配现用不能储存。任何溶解后的染料最好立即使用;无水的DMSO溶液-20°C保存最多2个星期。

1. Product introduction

Super Fluor 750 SE=Alexa Fluor 750 SE; Super Flour series (the effect is the same as Alexa Fluor series) fluorescent probes, with stronger fluorescence intensity, wider pH application range (pH 4~10), better resistance to quenching Sex. In the field of bioluminescence, it has gradually replaced FITC, Cy3, Cy5, Cy5.5, Cy7 and other fluorescent dyes.

Table Parameter of Super Fluor 488, 555, 647, 680, 750 activated ester

 

488

555

647

680

750

Molecular weight of activated ester

~900

 ~1250

 ~1300

~1150

 ~1300

Maximum absorption wavelength (nm)

494

555

647

680

750

Molar absorption coefficient (M-1cm-1)

71000

150000

239000

184000

240000

Maximum emission wavelength (nm)

524

565

665

702

775

A280nm/Amax

11%

8%

3%

5%

4%

 

2. Reference scheme of Super Fluor labeled antibody

1) Protein dissolution: Dissolve with 0.1 M NaHCO3 to a final concentration of 2mg/mL

Add 1ml of deionized water to an 84mg NaHCO3 bottle to make a 1M sodium bicarbonate solution. Shake or pipette until completely dissolved. The pH of the solution is about 8.3. It can be stored at 2-6°C for 2 weeks; if the antibody is a solution, dilute the antibody to 2mg/mL, and then add 1/10 volume of the above sodium bicarbonate solution; if the antibody is a lyophilized powder, add 1M After the sodium bicarbonate solution is diluted with 10 times deionized water to 0.1M, use it to dissolve the antibody into a 2mg/mL solution.

2) Dye dissolution: After the activated Super Flour SE dye solid powder is slowly warmed to room temperature in a desiccator from the refrigerator, the dye preparation concentration is 1 mg/mL with anhydrous DMSO.

3) Labeling: Slowly add an appropriate amount of dye solution to 1 mL of protein solution, (protein: dye molar ratio = 1:20-50; the mass ratio can be optimized within the following range of 10-200ug Super Flour SE per mg of IgG antibody). At the same time, slowly stir for 1 hour at room temperature in the dark. It is also possible to directly add the protein solution directly into the reagent bottle of the solid powder of the dye, and at the same time, slowly stir for 1 hour at room temperature in the dark.

4) Purification: After purifying the product by dialysis or Sephadex, the product is freeze-dried into a powder or stored in 0.01 M PBS/2mM sodium azide solution at -20°C and protected from light.

5) Storage: Store the labeled protein at 2~6℃, away from light. If the concentration of the purified protein conjugate is less than 1 mg/mL, add BSA or other stable proteins at 1-10 mg/ml. In the presence of 2mM sodium azide, it can be stored for several months at 2-6 degrees. For longer storage, you need to store at -20°C after packaging. Avoid repeated freezing and thawing, and store in the dark.

3. Calculate the mark ratio F/P value

For most antibodies such as IgG, the molar absorption coefficient is 203000, and the F/P value is between 4-9 is the most appropriate.

Super Flour 488 labeled protein F/P value calculation:

1) Dilute quantitatively a few purified coupled proteins with PBS with precise multiples, and measure the light absorption at 280nm and 494nm in a 1cm cuvette;

2) Calculate the protein concentration in the sample: protein molar concentration=(A280-(A494×0.11))×dilution multiple/203000

3) Calculate the labeling ratio: moles of dye bound per mole of protein = A494 × dilution factor / (71000 × protein molar concentration)

4. Stability and storage of reactive dyes

Please note: the unopened powder can be stored for 6 months in the dark and dry at -20℃. The aqueous solution can not be stored when used. Any dissolved dye is best used immediately; anhydrous DMSO solution is stored at -20°C for up to 2 weeks.

相关属性

储存温度 避光,-20°C储存,干燥
品牌 Jinpan