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Phospho-ERBB2(Y1127) Antibody 货号：AP3781j

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Phospho-ERBB2(Y1127) Antibody 货号：AP3781j

Phospho-ERBB2(Y1127) Antibody

货　　号：AP3781j

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-ERBB2(Y1127) Antibody AP3781j

Application  WB, IF, DB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    137910 Da

Gene ID 2064
Other Names
ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen=CD340; Flags: Precursor
Target/Specificity
This ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1127 of human ERBB2.
Dilution
WB~~1:5000
IF~~1:100
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
AP3781l Phospho-mouse ERBB2(Y1223) Antibody 100 µl  ￥   1,100.00 M DB In stock
AP3781m Phospho-ERBB2(Y877) Antibody 100 µl  ￥   1,100.00 H WB,DB In stock
AP3781o Phospho-mouse ERBB2(Y1197) Antibody 100 µl  ￥   1,100.00 M DB In stock
AP3781q Phospho-M ERBB2(Y1140) Antibody 100 µl  ￥   1,100.00 M WB,DB In stock
AP3781s Phospho-ERBB2(Y1005) Antibody 100 µl  ￥   1,100.00 H WB,IF,DB In stock
AP3781t Phospho-ERBB2(Y1196) Antibody 100 µl  ￥   1,100.00 H WB,IF,DB In stock

产品详细信息

Phospho-ERBB2(Y1127) Antibody AP3781j

Application  WB, IF, DB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    137910 Da

Gene ID 2064
Other Names
ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen=CD340; Flags: Precursor
Target/Specificity
This ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1127 of human ERBB2.
Dilution
WB~~1:5000
IF~~1:100
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
AP3781l Phospho-mouse ERBB2(Y1223) Antibody 100 µl  ￥   1,100.00 M DB In stock
AP3781m Phospho-ERBB2(Y877) Antibody 100 µl  ￥   1,100.00 H WB,DB In stock
AP3781o Phospho-mouse ERBB2(Y1197) Antibody 100 µl  ￥   1,100.00 M DB In stock
AP3781q Phospho-M ERBB2(Y1140) Antibody 100 µl  ￥   1,100.00 M WB,DB In stock
AP3781s Phospho-ERBB2(Y1005) Antibody 100 µl  ￥   1,100.00 H WB,IF,DB In stock
AP3781t Phospho-ERBB2(Y1196) Antibody 100 µl  ￥   1,100.00 H WB,IF,DB In stock

Phospho-SHP2(Y584) Antibody 货号：AP3716a

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Phospho-SHP2(Y584) Antibody 货号：AP3716a

Phospho-SHP2(Y584) Antibody

货　　号：AP3716a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-SHP2(Y584) Antibody  AP3716a

Application  DB, WB
Reactivity    Human
Predicted    Mouse, Rat
Isotype    Rabbit Ig
Calculated MW    68436 Da

Gene ID 5781
Other Names
PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3
Target/Specificity
This SHP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y584 of human SHP2.
Dilution
WB~~1:2000
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

产品详细信息

Phospho-SHP2(Y584) Antibody  AP3716a

Application  DB, WB
Reactivity    Human
Predicted    Mouse, Rat
Isotype    Rabbit Ig
Calculated MW    68436 Da

Gene ID 5781
Other Names
PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3
Target/Specificity
This SHP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y584 of human SHP2.
Dilution
WB~~1:2000
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Phospho-ERBB3(Y1289) Antibody 货号：AP3708a

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Phospho-ERBB3(Y1289) Antibody 货号：AP3708a

Phospho-ERBB3(Y1289) Antibody

货　　号：AP3708a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-ERBB3(Y1289) Antibody  AP3708a

Application  DB, WB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    148098 Da

Gene ID 2065
Other Names
ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3
Target/Specificity
This ERBB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1289 of human ERBB3.
Dilution
DB~~1:500
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

产品详细信息

Phospho-ERBB3(Y1289) Antibody  AP3708a

Application  DB, WB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    148098 Da

Gene ID 2065
Other Names
ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3
Target/Specificity
This ERBB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1289 of human ERBB3.
Dilution
DB~~1:500
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Phospho-TERT(Y707) Antibody 货号：AP3619a

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Phospho-TERT(Y707) Antibody 货号：AP3619a

Phospho-TERT(Y707) Antibody

货　　号：AP3619a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-TERT(Y707) Antibody  AP3619a

Application     WB, DB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    126997 Da

Gene ID 7015
Other Names
TERT; EST2; TCS1; TRT; Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2
Target/Specificity
This TERT Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y707 of human TERT.
Dilution
IF~~1:10~50
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

产品详细信息

Phospho-TERT(Y707) Antibody  AP3619a

Application     WB, DB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    126997 Da

Gene ID 7015
Other Names
TERT; EST2; TCS1; TRT; Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2
Target/Specificity
This TERT Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y707 of human TERT.
Dilution
IF~~1:10~50
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Phospho-EGFR(Y1016) Antibody 货号：AP3507a

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Phospho-EGFR(Y1016) Antibody 货号：AP3507a

Phospho-EGFR(Y1016) Antibody

货　　号：AP3507a

产品规格：100ul

原  产  地：USA

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-EGFR(Y1016) Antibody  AP3507a

Application     WB, DB
Reactivity    Human
Predicted    Mouse
Isotype    Rabbit Ig
Calculated MW    134277 Da

Gene ID 1956
Other Names
EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Target/Specificity
This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Dilution
WB~~1:8000
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

产品详细信息

Phospho-EGFR(Y1016) Antibody  AP3507a

Application     WB, DB
Reactivity    Human
Predicted    Mouse
Isotype    Rabbit Ig
Calculated MW    134277 Da

Gene ID 1956
Other Names
EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Target/Specificity
This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Dilution
WB~~1:8000
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Phospho-EGFR(Y1172) Antibody 货号：AP3407a

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Phospho-EGFR(Y1172) Antibody 货号：AP3407a

Phospho-EGFR(Y1172) Antibody

货　　号：AP3407a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-EGFR(Y1172) Antibody  AP3407a

Application     WB, DB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    134277 Da

Gene ID 1956
Other Names
EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Target/Specificity
This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1172 of human EGFR.
Dilution
WB~~1:1000
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
AP3407a Phospho-EGFR(Y1172) Antibody 100 µl  ￥   1,100.00 H WB,DB In stock
AP3411a Phospho-EGFR(Y1092) Antibody 100 µl  ￥   1,100.00 H DB,WB In stock

产品详细信息

Phospho-EGFR(Y1172) Antibody  AP3407a

Application     WB, DB
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    134277 Da

Gene ID 1956
Other Names
EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Target/Specificity
This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1172 of human EGFR.
Dilution
WB~~1:1000
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
AP3407a Phospho-EGFR(Y1172) Antibody 100 µl  ￥   1,100.00 H WB,DB In stock
AP3411a Phospho-EGFR(Y1092) Antibody 100 µl  ￥   1,100.00 H DB,WB In stock

Phospho-Gab1(Y659) Antibody 货号：AP3302b

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Phospho-Gab1(Y659) Antibody 货号：AP3302b

Phospho-Gab1(Y659) Antibody

货　　号：AP3302b

产品规格：100ul

原  产  地：USA

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-Gab1(Y659) Antibody  AP3302b

Application DB
Reactivity    Human
Predicted    Mouse, Bovine
Isotype    Rabbit Ig
Calculated MW    76616 Da

Gene ID 2549
Other Names
GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Target/Specificity
This Gab1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y659 of human Gab1.
Dilution
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

产品详细信息

Phospho-Gab1(Y659) Antibody  AP3302b

Application DB
Reactivity    Human
Predicted    Mouse, Bovine
Isotype    Rabbit Ig
Calculated MW    76616 Da

Gene ID 2549
Other Names
GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Target/Specificity
This Gab1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y659 of human Gab1.
Dilution
DB~~1:500
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Phospho-STAT5a(Y694) Antibody 货号：AP3268a

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Phospho-STAT5a(Y694) Antibody 货号：AP3268a

Phospho-STAT5a(Y694) Antibody

货　　号：AP3268a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-STAT5a(Y694) Antibody AP3268a

Application     WB, IF, IHC
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    90647 Da

Phospho-STAT5a(Y694) Antibody – Additional info

Gene ID 6776
Other Names
STAT5A; STAT5; Signal transducer and activator of transcription 5A
Target/Specificity
This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.
Dilution
WB~~1:1000
IF~~1:200
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
AP3256a Phospho-STAT1(S727) Antibody 100 µl  ￥   1,100.00 H WB,IHC 2-4 weeks
AP3260a Phospho-STAT3(S727) Antibody 100 µl  ￥   1,100.00 H, M IF,WB,IHC 2-4 weeks
AP3261a Phospho-STAT3(Y705) Antibody 100 µl  ￥   1,100.00 H WB,IHC 2-4 weeks
AP3266a Phospho-STAT5a(S726) Antibody 100 µl  ￥   1,100.00 H DB,WB,IHC 2-4 weeks
AP3268a Phospho-STAT5a(Y694) Antibody 100 µl  ￥   1,100.00 H WB,IF,IHC In stock

产品详细信息

Phospho-STAT5a(Y694) Antibody AP3268a

Application     WB, IF, IHC
Reactivity    Human
Isotype    Rabbit Ig
Calculated MW    90647 Da

Phospho-STAT5a(Y694) Antibody – Additional info

Gene ID 6776
Other Names
STAT5A; STAT5; Signal transducer and activator of transcription 5A
Target/Specificity
This STAT5a Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y694 of human STAT5a.
Dilution
WB~~1:1000
IF~~1:200
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
AP3256a Phospho-STAT1(S727) Antibody 100 µl  ￥   1,100.00 H WB,IHC 2-4 weeks
AP3260a Phospho-STAT3(S727) Antibody 100 µl  ￥   1,100.00 H, M IF,WB,IHC 2-4 weeks
AP3261a Phospho-STAT3(Y705) Antibody 100 µl  ￥   1,100.00 H WB,IHC 2-4 weeks
AP3266a Phospho-STAT5a(S726) Antibody 100 µl  ￥   1,100.00 H DB,WB,IHC 2-4 weeks
AP3268a Phospho-STAT5a(Y694) Antibody 100 µl  ￥   1,100.00 H WB,IF,IHC In stock

Phospho-SRC(Y215) Antibody 货号：AP3253a

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Phospho-SRC(Y215) Antibody 货号：AP3253a

Phospho-SRC(Y215) Antibody

货　　号：AP3253a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-SRC(Y215) Antibody AP3253a

Application WB
Reactivity    Human
Predicted    Mouse, Rat, Zebrafish, Chicken, Xenopus
Isotype    Rabbit Ig
Calculated MW    59835 Da

Gene ID 6714
Other Names
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src
Target/Specificity
This SRC Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y215 of human SRC.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

产品详细信息

Phospho-SRC(Y215) Antibody AP3253a

Application WB
Reactivity    Human
Predicted    Mouse, Rat, Zebrafish, Chicken, Xenopus
Isotype    Rabbit Ig
Calculated MW    59835 Da

Gene ID 6714
Other Names
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src
Target/Specificity
This SRC Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y215 of human SRC.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Phospho-IKKb(Y199) Antibody 货号：AP3127a

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Phospho-IKKb(Y199) Antibody 货号：AP3127a

Phospho-IKKb(Y199) Antibody

货　　号：AP3127a

产品规格：100ul

原  产  地：abgent

参考价格：1100 （参考价格，以实际价格为准）

优惠价格：

产品详细信息

Phospho-IKKb(Y199) Antibody  AP3127a

Application     WB
Reactivity    Human
Predicted    Mouse, Rat, Bovine
Isotype    Rabbit Ig
Calculated MW    86564 Da

Phospho-IKKb(Y199) Antibody – Additional info

Gene ID 3551
Other Names
IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
Target/Specificity
This IKKb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

产品详细信息

Phospho-IKKb(Y199) Antibody  AP3127a

Application     WB
Reactivity    Human
Predicted    Mouse, Rat, Bovine
Isotype    Rabbit Ig
Calculated MW    86564 Da

Phospho-IKKb(Y199) Antibody – Additional info

Gene ID 3551
Other Names
IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
Target/Specificity
This IKKb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.